Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC772_RS16305 Genome accession   NZ_CP061664
Coordinates   3520740..3521540 (-) Length   266 a.a.
NCBI ID   WP_191015832.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3515740..3526540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC772_RS16285 (IC772_16275) - 3516805..3517503 (-) 699 WP_002053293.1 YfgM family protein -
  IC772_RS16290 (IC772_16280) hisS 3517532..3518824 (-) 1293 WP_191015829.1 histidine--tRNA ligase -
  IC772_RS16295 (IC772_16285) ispG 3518821..3519936 (-) 1116 WP_191015831.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC772_RS16300 (IC772_16290) - 3519958..3520749 (-) 792 WP_004698761.1 helix-turn-helix domain-containing protein -
  IC772_RS16305 (IC772_16295) pilF 3520740..3521540 (-) 801 WP_191015832.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC772_RS16310 (IC772_16300) rlmN 3521556..3522788 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC772_RS16315 (IC772_16305) ndk 3522911..3523342 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC772_RS16320 (IC772_16310) iscX 3523456..3523656 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC772_RS16325 (IC772_16315) - 3523756..3524307 (-) 552 WP_134262245.1 phosphatase PAP2 family protein -
  IC772_RS16330 (IC772_16320) - 3524332..3525630 (-) 1299 WP_191017833.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30107.46 Da        Isoelectric Point: 9.2307

>NTDB_id=483937 IC772_RS16305 WP_191015832.1 3520740..3521540(-) (pilF) [Acinetobacter seifertii strain AS28]
MSTPKLKIMLCIGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483937 IC772_RS16305 WP_191015832.1 3520740..3521540(-) (pilF) [Acinetobacter seifertii strain AS28]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATTGGGGTTGCAGTTGCATTTCTGGCAAGTGGCTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.368

100

0.974
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726