Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   IC774_RS18475 Genome accession   NZ_CP061655
Coordinates   3782984..3785212 (+) Length   742 a.a.
NCBI ID   WP_151792277.1    Uniprot ID   A0A7H2SPG7
Organism   Acinetobacter seifertii strain AS33     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3777984..3790212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC774_RS18450 (IC774_18450) - 3779522..3779860 (-) 339 WP_077169111.1 tRNA-binding protein -
  IC774_RS18455 (IC774_18455) - 3780092..3780352 (+) 261 WP_019456904.1 DUF2798 domain-containing protein -
  IC774_RS18460 (IC774_18460) - 3780412..3780756 (-) 345 WP_019456903.1 hypothetical protein -
  IC774_RS18465 (IC774_18465) gspG 3781022..3781606 (-) 585 WP_171262331.1 type II secretion system major pseudopilin GspG -
  IC774_RS18470 (IC774_18470) gspF 3781630..3782835 (-) 1206 WP_077169113.1 type II secretion system inner membrane protein GspF -
  IC774_RS18475 (IC774_18475) priA 3782984..3785212 (+) 2229 WP_151792277.1 primosomal protein N' Machinery gene
  IC774_RS18480 (IC774_18480) - 3785288..3787129 (+) 1842 WP_077169115.1 monovalent cation:proton antiporter-2 (CPA2) family protein -
  IC774_RS18485 (IC774_18485) - 3787295..3788170 (+) 876 WP_004699009.1 Hsp33 family molecular chaperone HslO -
  IC774_RS18490 (IC774_18490) - 3788308..3788919 (+) 612 WP_004699012.1 LysE family translocator -
  IC774_RS18495 (IC774_18495) - 3789070..3790031 (+) 962 Protein_3646 DnaJ C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83997.87 Da        Isoelectric Point: 8.5588

>NTDB_id=483861 IC774_RS18475 WP_151792277.1 3782984..3785212(+) (priA) [Acinetobacter seifertii strain AS33]
MTITPKPDSVVYRVRVAVPVHLYDTFDYTLTKAQYERAQVGSRVAISFGRQNLIGIITEKVDPQESFTGTFQLKAISELL
DDEPILDEQVLSLLTWSAQYYQFPIGEVMQTALPALLRQGKPMDVLFHLWKIIPCDNVEALLKRSIKQQDAYQILKLHPA
GTTENILNLSGVETATLKALQKKGLIDCTLEPHDFSPAPVQLAQMPLTLNDDQKKATQHVINAQHQYQAFLLDGLTGSGK
TEVYLYIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFNCDIALLHSGLNDSKRLQAWQQAQTGKASIILGTRSAIYTP
LPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGHLQSCPVILGSATPSIDSYHLVETGKLTSLQLNQRAGHALLPKMH
LIDLKIVKKQHGISQPLIEQIKNTLARKEQVLIFLNRRGYAPVLVCESCGWQANCPHCDAHFTLHTQPYSYLHCHHCGTV
HRLPDHCPECQQKSLKTLGAGTAKVEEHLQELFPDYDVIRVDRDSTSRVGSWQKIYDRIHQNKPTILLGTQMLAKGHHFP
HVTLVAILDIDAGLLSVDIRAPERTAQLIVQVAGRAGRGEHKGHVYLQTLRPDHPLLTTLIEKDYRAVAKQTLAERKVAL
LPPYRYAVLIRAESKDRDYTLHFLNEAAEQLRQIAGDIVDIWGPIPAPMERKAGRYRAHMVILSADRARLHFYLRQWWAQ
LVHAPRQHQLRLSIDVDPQEFS

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=483861 IC774_RS18475 WP_151792277.1 3782984..3785212(+) (priA) [Acinetobacter seifertii strain AS33]
ATGACCATAACGCCAAAACCTGATTCAGTTGTTTATCGTGTGCGTGTTGCCGTACCTGTGCATCTGTACGACACTTTTGA
CTATACACTCACCAAAGCACAATATGAACGGGCTCAGGTAGGTTCGCGCGTCGCGATTTCATTTGGACGGCAAAACCTGA
TTGGTATTATTACCGAAAAAGTTGACCCACAAGAATCATTTACAGGCACCTTTCAGCTTAAAGCCATTTCTGAACTTTTA
GATGATGAACCTATTTTAGATGAACAAGTTTTAAGTTTATTGACATGGTCTGCCCAGTATTACCAATTTCCGATTGGTGA
AGTGATGCAAACGGCCCTACCCGCGTTATTGCGTCAGGGTAAACCAATGGATGTATTGTTTCATTTATGGAAAATCATTC
CTTGTGACAATGTTGAAGCCTTGTTAAAGCGTTCCATTAAGCAACAAGATGCATATCAGATTTTAAAATTGCATCCTGCT
GGAACTACAGAAAATATTCTTAATTTAAGTGGTGTAGAAACGGCTACACTTAAAGCGTTACAAAAGAAAGGACTCATAGA
CTGTACACTTGAGCCTCATGATTTTAGCCCAGCACCTGTGCAATTGGCTCAAATGCCACTTACGCTAAATGATGATCAAA
AGAAGGCAACTCAGCACGTCATTAATGCACAGCATCAATACCAAGCATTTTTACTAGATGGCTTAACTGGAAGTGGTAAA
ACTGAAGTTTATTTGTACATCATGCATGAGGTGTTAAAACAAGGTAAGCAAGTCCTAGTATTGGTTCCTGAAATTGGCTT
AACCCCTCAAACTATTTCGCGCTTTAAGTCTCGTTTTAACTGCGATATTGCTTTATTGCATTCAGGCTTAAATGACTCTA
AACGCTTACAAGCATGGCAACAGGCACAAACTGGCAAAGCCTCTATTATTTTAGGAACTCGCTCAGCCATTTATACGCCG
CTGCCACGTCTGGGCCTCATCATTTTAGATGAAGAACATGACCTGTCTTATAAACAGCAAGAAGGTTTCCGTTATCATGC
TCGTGATGTCGCACTGTACCGAGGCCACTTACAAAGCTGCCCTGTTATTTTAGGTTCGGCCACTCCAAGCATCGACAGCT
ATCACTTAGTAGAAACTGGTAAGTTAACTTCACTGCAACTGAACCAGCGTGCAGGCCACGCGCTTTTACCTAAAATGCAT
TTGATAGATCTTAAAATTGTCAAAAAGCAGCATGGAATTAGCCAGCCTTTAATCGAGCAAATTAAAAATACCCTAGCAAG
AAAAGAACAGGTTTTAATCTTTTTAAACCGTCGTGGCTATGCGCCTGTACTTGTGTGTGAAAGCTGTGGATGGCAAGCAA
ATTGCCCACATTGCGATGCGCATTTTACGTTGCATACTCAGCCTTATTCTTATTTACATTGTCACCACTGCGGCACAGTT
CATCGCTTGCCAGACCACTGCCCTGAGTGTCAGCAGAAGAGTTTAAAAACTTTAGGTGCAGGAACAGCCAAAGTTGAAGA
ACACTTACAAGAACTATTCCCAGATTATGATGTGATTCGTGTTGACCGTGACAGTACCAGTCGTGTCGGTAGCTGGCAAA
AAATCTATGATCGTATTCATCAAAACAAACCCACAATTTTGCTTGGTACTCAAATGTTGGCAAAGGGTCACCATTTTCCA
CATGTGACCTTGGTTGCTATTTTAGATATTGATGCGGGTTTACTGAGTGTCGATATTCGTGCGCCAGAACGTACGGCGCA
GTTGATTGTACAAGTGGCTGGTCGTGCAGGTCGTGGAGAACACAAAGGTCATGTTTATTTACAGACTTTAAGGCCAGATC
ATCCTTTACTCACCACACTCATCGAAAAAGATTATCGGGCGGTCGCGAAACAAACATTAGCTGAACGAAAAGTTGCATTA
CTGCCGCCTTATCGTTATGCAGTCTTAATTCGTGCTGAGTCGAAAGATCGTGACTATACCTTACATTTTTTAAATGAAGC
TGCCGAACAGCTTCGCCAAATTGCAGGTGATATTGTTGATATTTGGGGGCCGATTCCAGCACCAATGGAACGTAAAGCGG
GACGTTACCGCGCCCATATGGTGATTTTGTCTGCCGACCGTGCTCGTCTGCATTTTTACTTAAGACAATGGTGGGCTCAG
TTGGTTCATGCACCAAGACAGCATCAACTTAGACTCTCTATTGATGTTGACCCGCAAGAATTTAGTTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(214-372)

3DNA-binding domain (3BD) (PriA_3primeBD)

(15-114)

Primosomal protein N C-terminal domain (PriA_C)

(643-738)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2SPG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

97.305

100

0.973