Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC774_RS17795 Genome accession   NZ_CP061655
Coordinates   3642925..3643725 (-) Length   266 a.a.
NCBI ID   WP_190977850.1    Uniprot ID   A0A7H2QYU4
Organism   Acinetobacter seifertii strain AS33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3637925..3648725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC774_RS17775 (IC774_17775) - 3638990..3639688 (-) 699 WP_002053293.1 tetratricopeptide repeat protein -
  IC774_RS17780 (IC774_17780) hisS 3639717..3641009 (-) 1293 WP_077169052.1 histidine--tRNA ligase -
  IC774_RS17785 (IC774_17785) ispG 3641006..3642121 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC774_RS17790 (IC774_17790) - 3642143..3642934 (-) 792 WP_077169053.1 RodZ domain-containing protein -
  IC774_RS17795 (IC774_17795) pilF 3642925..3643725 (-) 801 WP_190977850.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC774_RS17800 (IC774_17800) rlmN 3643741..3644973 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC774_RS17805 (IC774_17805) ndk 3645096..3645527 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC774_RS17810 (IC774_17810) iscX 3645641..3645841 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC774_RS17815 (IC774_17815) - 3645941..3646493 (-) 553 Protein_3512 phosphatase PAP2 family protein -
  IC774_RS17820 (IC774_17820) - 3646518..3647816 (-) 1299 WP_077169054.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30109.44 Da        Isoelectric Point: 9.3755

>NTDB_id=483858 IC774_RS17795 WP_190977850.1 3642925..3643725(-) (pilF) [Acinetobacter seifertii strain AS33]
MSTPKLKIMLSMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483858 IC774_RS17795 WP_190977850.1 3642925..3643725(-) (pilF) [Acinetobacter seifertii strain AS33]
TTGAGTACTCCGAAGTTAAAGATCATGTTATCTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2QYU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.368

100

0.974
  pilF Acinetobacter baylyi ADP1

73.585

99.624

0.733