Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   IC776_RS16540 Genome accession   NZ_CP061646
Coordinates   3562016..3562441 (-) Length   141 a.a.
NCBI ID   WP_174743602.1    Uniprot ID   A0A7H2NMB1
Organism   Acinetobacter seifertii strain AS39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3557016..3567441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC776_RS16510 (IC776_16510) - 3557511..3558545 (+) 1035 WP_191012229.1 lipase secretion chaperone -
  IC776_RS16515 (IC776_16515) - 3558666..3559607 (+) 942 WP_081409789.1 lipase family alpha/beta hydrolase -
  IC776_RS16520 (IC776_16520) rplS 3559661..3560029 (-) 369 WP_002116654.1 50S ribosomal protein L19 -
  IC776_RS16525 (IC776_16525) trmD 3560249..3561004 (-) 756 WP_151682763.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  IC776_RS16530 (IC776_16530) rimM 3561050..3561598 (-) 549 WP_004705239.1 ribosome maturation factor RimM -
  IC776_RS16535 (IC776_16535) rpsP 3561618..3561869 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  IC776_RS16540 (IC776_16540) pilE 3562016..3562441 (-) 426 WP_174743602.1 type IV pilin protein Machinery gene
  IC776_RS16545 (IC776_16545) pilY2 3562438..3562920 (-) 483 WP_191012230.1 type IV pilin protein Machinery gene
  IC776_RS16550 (IC776_16550) pilY1 3562933..3567117 (-) 4185 WP_191012231.1 PilC/PilY family type IV pilus protein Machinery gene

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15286.49 Da        Isoelectric Point: 7.7765

>NTDB_id=483823 IC776_RS16540 WP_174743602.1 3562016..3562441(-) (pilE) [Acinetobacter seifertii strain AS39]
MKNGFTLIEIMIVVAIIAILVAIATPSYLQYVRKGNRTAVQSEMMNIAQNLESQKMVNNRYPSNATIQSIYGSNVSPLQG
KALYTLAFTTLNDSTWVLTATPITNTSQAGDGIICLNDQGQKFWAKGATDCALSASSSWTE

Nucleotide


Download         Length: 426 bp        

>NTDB_id=483823 IC776_RS16540 WP_174743602.1 3562016..3562441(-) (pilE) [Acinetobacter seifertii strain AS39]
GTGAAGAATGGTTTTACACTGATAGAAATTATGATTGTGGTGGCAATTATTGCAATTTTGGTAGCGATAGCCACTCCATC
GTATTTACAATATGTTCGTAAAGGGAATCGTACAGCCGTTCAATCTGAAATGATGAATATTGCACAAAATTTAGAGTCTC
AAAAAATGGTGAATAACCGTTATCCCTCAAATGCAACTATACAATCAATTTATGGCTCTAATGTGAGTCCTCTTCAGGGC
AAAGCTTTATATACTTTAGCTTTTACTACTTTAAACGATTCGACATGGGTGCTTACGGCAACACCAATCACTAATACTTC
GCAAGCGGGTGATGGAATTATTTGCTTAAATGATCAAGGGCAAAAATTTTGGGCAAAAGGTGCTACAGATTGCGCACTAT
CGGCTTCATCAAGTTGGACAGAGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(26-115)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2NMB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

96.454

100

0.965
  comF Acinetobacter baylyi ADP1

53.571

99.291

0.532