Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC781_RS02630 Genome accession   NZ_CP061626
Coordinates   565988..566788 (+) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560988..571788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC781_RS02605 (IC781_02605) - 561898..563196 (+) 1299 WP_081400363.1 glycosyltransferase family 4 protein -
  IC781_RS02610 (IC781_02610) - 563221..563772 (+) 552 WP_019458079.1 phosphatase PAP2 family protein -
  IC781_RS02615 (IC781_02615) iscX 563872..564072 (+) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC781_RS02620 (IC781_02620) ndk 564186..564617 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC781_RS02625 (IC781_02625) rlmN 564740..565972 (+) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC781_RS02630 (IC781_02630) pilF 565988..566788 (+) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC781_RS02635 (IC781_02635) - 566779..567570 (+) 792 WP_004698761.1 helix-turn-helix domain-containing protein -
  IC781_RS02640 (IC781_02640) ispG 567592..568707 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC781_RS02645 (IC781_02645) hisS 568704..569996 (+) 1293 WP_191010809.1 histidine--tRNA ligase -
  IC781_RS02650 (IC781_02650) - 570025..570723 (+) 699 WP_002053293.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=483640 IC781_RS02630 WP_004698762.1 565988..566788(+) (pilF) [Acinetobacter seifertii strain AS48]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483640 IC781_RS02630 WP_004698762.1 565988..566788(+) (pilF) [Acinetobacter seifertii strain AS48]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAGCAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726