Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AVCA_RS13270 Genome accession   NC_021149
Coordinates   3006061..3007767 (-) Length   568 a.a.
NCBI ID   WP_012701471.1    Uniprot ID   C1DLZ7
Organism   Azotobacter vinelandii CA     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3001061..3012767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVCA_RS13240 (AvCA_29090) - 3001375..3001857 (-) 483 WP_012701465.1 type II secretion system protein -
  AVCA_RS13245 (AvCA_29100) - 3001854..3003698 (-) 1845 WP_012701466.1 secretin N-terminal domain-containing protein -
  AVCA_RS13250 (AvCA_29110) - 3003695..3004222 (-) 528 WP_012701467.1 hypothetical protein -
  AVCA_RS13255 (AvCA_29120) - 3004222..3004770 (-) 549 WP_012701468.1 GspMb/PilO family protein -
  AVCA_RS13260 (AvCA_29130) - 3004757..3005299 (-) 543 WP_012701469.1 hypothetical protein -
  AVCA_RS13265 (AvCA_29140) - 3005301..3006080 (-) 780 WP_236616163.1 hypothetical protein -
  AVCA_RS13270 (AvCA_29150) pilF 3006061..3007767 (-) 1707 WP_012701471.1 GspE/PulE family protein Machinery gene
  AVCA_RS13275 (AvCA_29160) - 3007809..3008189 (-) 381 WP_012701472.1 response regulator -
  AVCA_RS13280 (AvCA_29170) - 3008778..3009020 (+) 243 WP_012701473.1 hypothetical protein -
  AVCA_RS13285 (AvCA_29180) - 3009045..3009629 (-) 585 WP_012701474.1 6-pyruvoyl trahydropterin synthase family protein -
  AVCA_RS13290 (AvCA_29190) - 3009623..3009982 (-) 360 WP_236616164.1 hypothetical protein -
  AVCA_RS13295 (AvCA_29200) - 3010088..3010648 (-) 561 WP_012701476.1 carbonate dehydratase -
  AVCA_RS13300 (AvCA_29210) folE2 3010838..3011734 (-) 897 WP_012701477.1 GTP cyclohydrolase FolE2 -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61893.76 Da        Isoelectric Point: 5.0379

>NTDB_id=48362 AVCA_RS13270 WP_012701471.1 3006061..3007767(-) (pilF) [Azotobacter vinelandii CA]
MDNLSPEHAADGAPRDEGRFAAELLCEARERAARDGGRALEALETLAGLAPEAFVRRLGATLHYPVLVGETLFACRPAFE
RLALAQALKREFAVVRLDEREVGVFADPFDDDRLAWIDACLEGAPLYLVHPADLAGYLARHEEAFQAVESLGADAAGTVE
AEAVENLSLARISEDSSVVVKLVNSTLYDALKLHASDIHLGMTGNGLVIKYRIDGVLNSIGRVQGSETADQVISRIKVMA
ELDIGEKRVPQDGRFKVGIHGRQIDFRVSIMPSIFGEDAVLRVLDKQDLADRVSGVSLEALGFEEATLRNLRRLAAEPYG
MVLVTGPTGSGKTTTLYAMITEINHGVDKIITIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDP
ETAQIAVQSALTGHLVFTTIHANNVFDVIGRFVQMEVDPYSFVSALNAVLAQRLVRLVCPDCAAPVEPGEEELLTSGLDP
AGVGDFGFVHGSGCGRCRGSGYRGRKAIAELLLLDDEIRQMIVERQPITRVKELARRRGLRLLRDSALELVRSGRTSLEE
INRVTFAG

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=48362 AVCA_RS13270 WP_012701471.1 3006061..3007767(-) (pilF) [Azotobacter vinelandii CA]
ATGGACAATCTTTCTCCGGAACATGCGGCCGACGGGGCGCCGCGGGATGAGGGCCGTTTCGCGGCGGAGCTGCTGTGCGA
GGCCCGCGAGCGCGCGGCGCGCGACGGTGGGCGGGCGCTGGAGGCGCTGGAGACGCTGGCCGGGCTGGCGCCGGAGGCGT
TCGTCCGCCGTCTGGGGGCGACCCTGCATTATCCGGTGCTGGTCGGCGAGACCCTGTTCGCCTGCCGTCCGGCATTCGAG
CGGCTGGCGCTGGCCCAGGCGCTCAAGCGCGAATTCGCCGTGGTGCGCCTGGACGAGCGCGAGGTCGGGGTGTTCGCCGA
TCCCTTCGACGACGACCGCCTGGCCTGGATCGACGCCTGCCTGGAGGGCGCGCCGCTGTATCTGGTGCATCCGGCCGACC
TGGCCGGCTACCTGGCGCGCCACGAGGAGGCCTTCCAGGCCGTCGAGTCGCTGGGCGCCGACGCCGCCGGGACGGTCGAG
GCCGAGGCGGTGGAGAATCTGTCGCTGGCGCGGATCAGCGAGGACTCCAGCGTGGTGGTCAAGCTGGTCAACTCGACCCT
CTACGACGCGCTGAAGTTGCACGCCAGCGACATCCACCTGGGCATGACCGGCAACGGCCTGGTGATCAAGTACCGCATCG
ACGGGGTGCTCAACTCCATCGGCCGGGTGCAGGGCAGCGAGACCGCCGACCAGGTGATCTCGCGGATCAAGGTGATGGCC
GAGCTGGACATCGGCGAGAAGCGGGTGCCCCAGGACGGTCGCTTCAAGGTGGGTATCCACGGCCGGCAGATCGACTTCCG
GGTGTCGATCATGCCGAGCATCTTCGGCGAGGACGCGGTACTGCGGGTGCTCGACAAGCAGGACCTGGCCGACCGCGTCA
GCGGCGTCAGCCTGGAGGCGCTGGGCTTCGAGGAAGCGACCCTGCGCAATCTGCGCCGGCTGGCCGCCGAACCCTACGGC
ATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACCACCACCCTGTACGCGATGATCACCGAGATCAATCACGGGGT
GGACAAGATCATCACCATCGAGGACCCGGTGGAATACCAGTTGCCGGGGGTGCTGCAGATCCCGGTCAACGAGAAGAAGG
GCCTGACCTTCGCCCGCGGCCTGCGCTCGATCCTGCGCCACGACCCGGACAAGATCATGGTCGGTGAGATCCGCGACCCG
GAAACCGCGCAGATCGCCGTGCAGTCGGCGCTCACCGGCCACCTGGTGTTCACCACCATCCACGCCAACAACGTGTTCGA
CGTGATCGGCCGCTTCGTGCAGATGGAGGTCGATCCCTACAGCTTCGTCTCCGCGCTCAACGCGGTGCTCGCCCAGCGCC
TGGTGCGCCTGGTCTGCCCGGACTGCGCCGCGCCGGTCGAGCCGGGCGAGGAGGAACTGCTGACTTCCGGGCTGGACCCG
GCCGGAGTCGGCGATTTCGGTTTCGTCCACGGCAGCGGCTGCGGGCGCTGCCGTGGCTCGGGCTATCGCGGGCGCAAGGC
GATCGCCGAGTTGCTGCTGCTCGACGACGAGATCCGCCAGATGATCGTCGAACGCCAGCCGATCACCCGGGTCAAGGAAC
TGGCCCGCCGCCGTGGTCTGCGCCTGCTGCGCGATTCGGCGCTGGAACTGGTGCGCAGCGGGCGAACCAGCCTGGAGGAG
ATCAACCGTGTCACTTTCGCGGGCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(186-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1DLZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

39.56

96.127

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.684

95.775

0.361

Multiple sequence alignment