Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC785_RS17870 Genome accession   NZ_CP061603
Coordinates   3664457..3665257 (-) Length   266 a.a.
NCBI ID   WP_190977850.1    Uniprot ID   A0A7H2QYU4
Organism   Acinetobacter seifertii strain AS53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3659457..3670257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC785_RS17850 (IC785_17850) - 3660522..3661220 (-) 699 WP_002053293.1 YfgM family protein -
  IC785_RS17855 (IC785_17855) hisS 3661249..3662541 (-) 1293 WP_077169052.1 histidine--tRNA ligase -
  IC785_RS17860 (IC785_17860) ispG 3662538..3663653 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC785_RS17865 (IC785_17865) - 3663675..3664466 (-) 792 WP_077169053.1 helix-turn-helix domain-containing protein -
  IC785_RS17870 (IC785_17870) pilF 3664457..3665257 (-) 801 WP_190977850.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC785_RS17875 (IC785_17875) rlmN 3665273..3666505 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC785_RS17880 (IC785_17880) ndk 3666628..3667059 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC785_RS17885 (IC785_17885) iscX 3667173..3667373 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC785_RS17890 (IC785_17890) - 3667473..3668024 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  IC785_RS17895 (IC785_17895) - 3668049..3669347 (-) 1299 WP_077169054.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30109.44 Da        Isoelectric Point: 9.3755

>NTDB_id=483528 IC785_RS17870 WP_190977850.1 3664457..3665257(-) (pilF) [Acinetobacter seifertii strain AS53]
MSTPKLKIMLSMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483528 IC785_RS17870 WP_190977850.1 3664457..3665257(-) (pilF) [Acinetobacter seifertii strain AS53]
TTGAGTACTCCGAAGTTAAAGATCATGTTATCTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2QYU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.368

100

0.974
  pilF Acinetobacter baylyi ADP1

73.585

99.624

0.733