Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC788_RS15705 Genome accession   NZ_CP061596
Coordinates   3338595..3339395 (-) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3333595..3344395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC788_RS15685 (IC788_15685) - 3334660..3335358 (-) 699 WP_002053293.1 YfgM family protein -
  IC788_RS15690 (IC788_15690) hisS 3335387..3336679 (-) 1293 WP_191000734.1 histidine--tRNA ligase -
  IC788_RS15695 (IC788_15695) ispG 3336676..3337791 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC788_RS15700 (IC788_15700) - 3337813..3338604 (-) 792 WP_004698761.1 helix-turn-helix domain-containing protein -
  IC788_RS15705 (IC788_15705) pilF 3338595..3339395 (-) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC788_RS15710 (IC788_15710) rlmN 3339411..3340643 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC788_RS15715 (IC788_15715) ndk 3340766..3341197 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC788_RS15720 (IC788_15720) iscX 3341311..3341511 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC788_RS15725 (IC788_15725) - 3341611..3342162 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  IC788_RS15730 (IC788_15730) - 3342187..3343485 (-) 1299 WP_100192007.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=483449 IC788_RS15705 WP_004698762.1 3338595..3339395(-) (pilF) [Acinetobacter seifertii strain AS59]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483449 IC788_RS15705 WP_004698762.1 3338595..3339395(-) (pilF) [Acinetobacter seifertii strain AS59]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGACAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTTCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATA
GCAAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726