Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC789_RS02655 Genome accession   NZ_CP061592
Coordinates   563209..564009 (+) Length   266 a.a.
NCBI ID   WP_190977850.1    Uniprot ID   A0A7H2QYU4
Organism   Acinetobacter seifertii strain AS60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558209..569009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC789_RS02630 (IC789_02625) - 559119..560417 (+) 1299 WP_077169054.1 glycosyltransferase family 4 protein -
  IC789_RS02635 (IC789_02630) - 560442..560993 (+) 552 WP_190977849.1 phosphatase PAP2 family protein -
  IC789_RS02640 (IC789_02635) iscX 561093..561293 (+) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC789_RS02645 (IC789_02640) ndk 561407..561838 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC789_RS02650 (IC789_02645) rlmN 561961..563193 (+) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC789_RS02655 (IC789_02650) pilF 563209..564009 (+) 801 WP_190977850.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC789_RS02660 (IC789_02655) - 564000..564791 (+) 792 WP_077169053.1 helix-turn-helix domain-containing protein -
  IC789_RS02665 (IC789_02660) ispG 564813..565928 (+) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC789_RS02670 (IC789_02665) hisS 565925..567217 (+) 1293 WP_077169052.1 histidine--tRNA ligase -
  IC789_RS02675 (IC789_02670) - 567246..567944 (+) 699 WP_002053293.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30109.44 Da        Isoelectric Point: 9.3755

>NTDB_id=483395 IC789_RS02655 WP_190977850.1 563209..564009(+) (pilF) [Acinetobacter seifertii strain AS60]
MSTPKLKIMLSMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483395 IC789_RS02655 WP_190977850.1 563209..564009(+) (pilF) [Acinetobacter seifertii strain AS60]
TTGAGTACTCCGAAGTTAAAGATCATGTTATCTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2QYU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.368

100

0.974
  pilF Acinetobacter baylyi ADP1

73.585

99.624

0.733