Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC796_RS16785 Genome accession   NZ_CP061561
Coordinates   3431762..3432562 (-) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3426762..3437562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC796_RS16765 (IC796_16760) - 3427827..3428525 (-) 699 WP_002053293.1 YfgM family protein -
  IC796_RS16770 (IC796_16765) hisS 3428554..3429846 (-) 1293 WP_151682914.1 histidine--tRNA ligase -
  IC796_RS16775 (IC796_16770) ispG 3429843..3430958 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC796_RS16780 (IC796_16775) - 3430980..3431771 (-) 792 WP_004698761.1 helix-turn-helix domain-containing protein -
  IC796_RS16785 (IC796_16780) pilF 3431762..3432562 (-) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC796_RS16790 (IC796_16785) rlmN 3432578..3433810 (-) 1233 WP_151682915.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC796_RS16795 (IC796_16790) ndk 3433933..3434364 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC796_RS16800 (IC796_16795) iscX 3434478..3434678 (-) 201 WP_151682916.1 Fe-S cluster assembly protein IscX -
  IC796_RS16805 (IC796_16800) - 3434778..3435329 (-) 552 WP_151682917.1 phosphatase PAP2 family protein -
  IC796_RS16810 (IC796_16805) - 3435354..3436652 (-) 1299 WP_151683002.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=483213 IC796_RS16785 WP_004698762.1 3431762..3432562(-) (pilF) [Acinetobacter seifertii strain AS73]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483213 IC796_RS16785 WP_004698762.1 3431762..3432562(-) (pilF) [Acinetobacter seifertii strain AS73]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATTGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726