Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC797_RS15855 Genome accession   NZ_CP061556
Coordinates   3342708..3343508 (-) Length   266 a.a.
NCBI ID   WP_191105691.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3337708..3348508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC797_RS15835 (IC797_15835) - 3338774..3339472 (-) 699 WP_191105690.1 tetratricopeptide repeat protein -
  IC797_RS15840 (IC797_15840) hisS 3339501..3340793 (-) 1293 WP_068932552.1 histidine--tRNA ligase -
  IC797_RS15845 (IC797_15845) ispG 3340790..3341905 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC797_RS15850 (IC797_15850) - 3341927..3342717 (-) 791 Protein_3116 helix-turn-helix domain-containing protein -
  IC797_RS15855 (IC797_15855) pilF 3342708..3343508 (-) 801 WP_191105691.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC797_RS15860 (IC797_15860) rlmN 3343524..3344756 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC797_RS15865 (IC797_15865) ndk 3344879..3345310 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC797_RS15870 (IC797_15870) iscX 3345424..3345624 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC797_RS15875 (IC797_15875) - 3345724..3346275 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  IC797_RS15880 (IC797_15880) - 3346300..3347598 (-) 1299 WP_191106373.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30153.55 Da        Isoelectric Point: 9.2307

>NTDB_id=483176 IC797_RS15855 WP_191105691.1 3342708..3343508(-) (pilF) [Acinetobacter seifertii strain AS74]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLVAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483176 IC797_RS15855 WP_191105691.1 3342708..3343508(-) (pilF) [Acinetobacter seifertii strain AS74]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGTTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTTCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(43-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.368

100

0.974
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722