Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IC799_RS15525 Genome accession   NZ_CP061550
Coordinates   3360429..3361229 (-) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS79     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3355429..3366229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC799_RS15505 (IC799_15500) - 3356494..3357192 (-) 699 WP_002053293.1 YfgM family protein -
  IC799_RS15510 (IC799_15505) hisS 3357221..3358513 (-) 1293 WP_191012573.1 histidine--tRNA ligase -
  IC799_RS15515 (IC799_15510) ispG 3358510..3359625 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IC799_RS15520 (IC799_15515) - 3359647..3360438 (-) 792 WP_004698761.1 helix-turn-helix domain-containing protein -
  IC799_RS15525 (IC799_15520) pilF 3360429..3361229 (-) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IC799_RS15530 (IC799_15525) rlmN 3361245..3362477 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IC799_RS15535 (IC799_15530) ndk 3362600..3363031 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IC799_RS15540 (IC799_15535) iscX 3363145..3363345 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IC799_RS15545 (IC799_15540) - 3363445..3363996 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  IC799_RS15550 (IC799_15545) - 3364021..3365319 (-) 1299 WP_191029219.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=483094 IC799_RS15525 WP_004698762.1 3360429..3361229(-) (pilF) [Acinetobacter seifertii strain AS79]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=483094 IC799_RS15525 WP_004698762.1 3360429..3361229(-) (pilF) [Acinetobacter seifertii strain AS79]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCAGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACACTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAACGTTATAATGATGCCGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726