Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IC807_RS08335 Genome accession   NZ_CP061470
Coordinates   1664840..1665913 (-) Length   357 a.a.
NCBI ID   WP_015375397.1    Uniprot ID   L7ZU71
Organism   Geobacillus zalihae strain SURF-189     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1659840..1670913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC807_RS08295 (IC807_08295) - 1660567..1661361 (+) 795 WP_020753848.1 YqhG family protein -
  IC807_RS08300 (IC807_08300) - 1661464..1661643 (-) 180 WP_011231910.1 YqzE family protein -
  IC807_RS08305 (IC807_08305) comGG 1661689..1662078 (-) 390 WP_012820555.1 competence type IV pilus minor pilin ComGG -
  IC807_RS08310 (IC807_08310) comGF 1662154..1662600 (-) 447 WP_033004723.1 competence type IV pilus minor pilin ComGF -
  IC807_RS08315 (IC807_08315) - 1662579..1662905 (-) 327 WP_020753845.1 type II secretion system protein -
  IC807_RS08320 (IC807_08320) comGD 1662889..1663326 (-) 438 WP_033004731.1 competence type IV pilus minor pilin ComGD -
  IC807_RS08325 (IC807_08325) comGC 1663316..1663612 (-) 297 WP_015375395.1 competence type IV pilus major pilin ComGC Machinery gene
  IC807_RS08330 (IC807_08330) comGB 1663815..1664843 (-) 1029 WP_012820550.1 competence type IV pilus assembly protein ComGB -
  IC807_RS08335 (IC807_08335) comGA 1664840..1665913 (-) 1074 WP_015375397.1 competence type IV pilus ATPase ComGA Machinery gene
  IC807_RS08340 (IC807_08340) - 1666106..1666804 (+) 699 WP_020753839.1 metalloregulator ArsR/SmtB family transcription factor -
  IC807_RS08345 (IC807_08345) - 1666960..1667202 (+) 243 WP_008879854.1 DUF2626 family protein -
  IC807_RS08350 (IC807_08350) - 1667286..1668419 (-) 1134 WP_081132472.1 class I SAM-dependent methyltransferase -
  IC807_RS08355 (IC807_08355) - 1668891..1669523 (-) 633 WP_012820544.1 MBL fold metallo-hydrolase -
  IC807_RS08360 (IC807_08360) - 1669692..1669868 (+) 177 WP_011231921.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39640.10 Da        Isoelectric Point: 8.1836

>NTDB_id=482370 IC807_RS08335 WP_015375397.1 1664840..1665913(-) (comGA) [Geobacillus zalihae strain SURF-189]
MLNEIEQTANRLLAEAVQRRASDLHLVPRRRDAAVRLRLDGMLVDVGALPKETAERLIVHFKFLAGMDIGERRRPQSGAM
EVAEFGETVYLRLSTLPTLYDESLVIRLLPQRLSLPLRELSLFFHSTARLFSFMQHPQGLVLLTGPTGSGKTTTLYTLLD
LCQAERQRNIITLEDPIEKQNERLLQVQINEKAGITYAAGLKAALRHDPDVLMVGEIRDHDTAAIAIRSALSGHLVVSTM
HAADAVGAVYRLHEFGIPLGDLAETLLAVSAQRLVELCCPLCGDDCHPSCGRLGRRRRTAVYELLCGSALEDVIHFLSNG
RGKPKRSYMTLARLIRKGIALGYLPVRMLELVGGEER

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=482370 IC807_RS08335 WP_015375397.1 1664840..1665913(-) (comGA) [Geobacillus zalihae strain SURF-189]
GTGCTGAACGAGATCGAACAAACGGCAAACCGCCTCCTCGCTGAAGCGGTGCAGCGCCGCGCCTCGGATCTCCACCTCGT
TCCGCGCCGCCGTGATGCCGCCGTTCGTCTTCGGCTCGATGGCATGCTTGTCGACGTTGGCGCTCTTCCGAAAGAAACGG
CGGAACGCCTCATCGTTCACTTTAAATTTTTAGCCGGCATGGACATCGGGGAACGACGCCGTCCGCAAAGCGGCGCCATG
GAAGTAGCGGAGTTTGGGGAAACGGTGTATTTGCGTTTATCGACATTGCCGACGCTCTACGATGAAAGCCTCGTCATTCG
ACTTCTGCCGCAGCGCCTCTCGCTGCCGCTTCGCGAACTATCCTTATTCTTCCACTCCACCGCACGATTATTTTCCTTCA
TGCAGCATCCGCAAGGACTCGTGCTGTTGACCGGTCCGACGGGGTCAGGAAAAACGACGACCCTGTACACCCTTCTTGAT
CTTTGTCAAGCGGAAAGGCAGCGCAATATCATCACGCTTGAAGATCCAATCGAAAAACAGAACGAACGACTGTTGCAAGT
GCAAATCAATGAAAAAGCAGGCATCACGTACGCAGCTGGGTTAAAAGCGGCGCTGCGTCATGACCCGGATGTGTTGATGG
TCGGCGAGATCCGCGATCATGACACGGCGGCCATTGCCATCCGTTCAGCATTGAGCGGCCATTTGGTCGTATCGACGATG
CACGCCGCCGATGCAGTCGGCGCCGTCTACCGGCTGCATGAATTCGGCATTCCGCTCGGCGATTTGGCTGAGACGCTGCT
TGCCGTCTCTGCCCAGCGGCTTGTCGAGCTTTGCTGCCCGTTGTGCGGCGACGATTGCCATCCATCGTGCGGCCGTCTTG
GGCGCCGCCGGCGCACGGCTGTTTATGAGTTGCTTTGCGGTTCAGCCCTGGAGGACGTGATTCATTTTCTCTCCAATGGG
AGAGGGAAGCCCAAGCGCTCTTATATGACGCTCGCTCGCTTGATCCGCAAGGGGATTGCGCTCGGCTACCTGCCCGTCCG
CATGCTTGAGCTCGTCGGAGGGGAAGAAAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L7ZU71

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

50.581

96.359

0.487