Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ICI50_RS09250 Genome accession   NZ_CP061341
Coordinates   1885479..1887275 (+) Length   598 a.a.
NCBI ID   WP_188891590.1    Uniprot ID   -
Organism   Lactobacillus kefiranofaciens strain 1207     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1862053..1890518 1885479..1887275 within 0


Gene organization within MGE regions


Location: 1862053..1890518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICI50_RS09130 (ICI50_09125) - 1862053..1863060 (+) 1008 WP_188891574.1 CAP domain-containing protein -
  ICI50_RS09135 (ICI50_09130) - 1863331..1864656 (-) 1326 WP_188891536.1 RNA-guided endonuclease TnpB family protein -
  ICI50_RS09140 (ICI50_09135) - 1864686..1865294 (-) 609 WP_188891575.1 IS607 family transposase -
  ICI50_RS09145 (ICI50_09140) - 1865563..1866513 (-) 951 WP_188891576.1 C40 family peptidase -
  ICI50_RS09150 (ICI50_09145) - 1866671..1867453 (-) 783 WP_188891577.1 C40 family peptidase -
  ICI50_RS09155 (ICI50_09150) - 1867637..1869025 (-) 1389 Protein_1767 ATP-binding protein -
  ICI50_RS09160 (ICI50_09155) - 1869297..1870568 (-) 1272 WP_188891578.1 RNA-guided endonuclease TnpB family protein -
  ICI50_RS09165 (ICI50_09160) - 1870803..1871357 (-) 555 WP_013854769.1 C40 family peptidase -
  ICI50_RS09170 (ICI50_09165) - 1871543..1872088 (-) 546 WP_188891579.1 AAA family ATPase -
  ICI50_RS09175 (ICI50_09170) - 1872184..1872495 (+) 312 WP_188891580.1 DUF2187 family protein -
  ICI50_RS09180 (ICI50_09175) - 1872588..1874099 (+) 1512 WP_188891581.1 O-antigen ligase family protein -
  ICI50_RS09185 (ICI50_09180) - 1874154..1874774 (-) 621 WP_188891582.1 LVIS_2131 family protein -
  ICI50_RS09190 (ICI50_09185) nrdI 1874883..1875328 (+) 446 Protein_1774 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  ICI50_RS09195 (ICI50_09190) - 1875331..1876266 (+) 936 WP_013854775.1 ribonucleotide-diphosphate reductase subunit beta -
  ICI50_RS09200 (ICI50_09195) - 1876311..1876865 (-) 555 WP_013854776.1 hypothetical protein -
  ICI50_RS09205 (ICI50_09200) - 1877104..1878429 (-) 1326 WP_188891536.1 RNA-guided endonuclease TnpB family protein -
  ICI50_RS09210 (ICI50_09205) - 1878459..1879067 (-) 609 WP_188891584.1 IS607 family transposase -
  ICI50_RS09215 (ICI50_09210) - 1879442..1879927 (-) 486 WP_013854777.1 threonine/serine exporter family protein -
  ICI50_RS09220 (ICI50_09215) - 1879930..1880700 (-) 771 WP_188891586.1 threonine/serine exporter family protein -
  ICI50_RS09225 (ICI50_09220) - 1880711..1882252 (-) 1542 WP_188891588.1 ABC-F family ATP-binding cassette domain-containing protein -
  ICI50_RS09230 (ICI50_09225) - 1882259..1882630 (-) 372 WP_025084257.1 hypothetical protein -
  ICI50_RS09235 (ICI50_09230) - 1882776..1883354 (+) 579 WP_013854781.1 GNAT family protein -
  ICI50_RS09240 (ICI50_09235) - 1883329..1884018 (-) 690 WP_013854782.1 DUF554 domain-containing protein -
  ICI50_RS09245 (ICI50_09240) - 1884088..1885317 (-) 1230 WP_054640592.1 LCP family protein -
  ICI50_RS09250 (ICI50_09245) pepF 1885479..1887275 (+) 1797 WP_188891590.1 oligoendopeptidase F Regulator
  ICI50_RS09255 (ICI50_09250) - 1887341..1887574 (-) 234 WP_025084256.1 bacteriocin immunity protein -
  ICI50_RS09260 (ICI50_09255) - 1887549..1887746 (-) 198 WP_013854786.1 helix-turn-helix transcriptional regulator -
  ICI50_RS09265 (ICI50_09260) - 1888219..1889538 (-) 1320 WP_013851273.1 RNA-guided endonuclease TnpB family protein -
  ICI50_RS09270 (ICI50_09265) tnpA 1889571..1889981 (-) 411 WP_188891539.1 IS200/IS605 family transposase -
  ICI50_RS09275 (ICI50_09270) - 1890183..1890347 (-) 165 WP_013854793.1 hypothetical protein -
  ICI50_RS09280 (ICI50_09275) - 1890357..1890518 (-) 162 WP_013854794.1 hypothetical protein -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68150.06 Da        Isoelectric Point: 4.7657

>NTDB_id=481997 ICI50_RS09250 WP_188891590.1 1885479..1887275(+) (pepF) [Lactobacillus kefiranofaciens strain 1207]
MAIPTRDEVPAELKWDLTRIFKTDQDWEQAYDDTKQEVAELDQLKGSLAKSGQDLYEGLTKILAVRRKVENIYVYATMSS
DVDTANSHYLGYVSRVQSLANQFEAVTSFISPEILSIPEDKFKQFLQAEPRLKNYAHYLETITNKRPHTLPAEEEKIIAD
AGDALSTSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYTTYGQFQNSLASTLSGVVKK
HNYNARVHKYNSAREAALAENGVPTKVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGKPSLSYNFKEA
KEVAKKALAPLGEDYLKHVDYIFNNRVIDSVENKNKVTGAYSGGAYGTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
HTQPYIYGEYPIFVAEIASTTNENILTEYFLGHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADAKGEPLTA
DTLDEVYGQINQHYYGDAVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTTAERDAYLGFLKAGSSDY
PTEIMKHAGVDMTKPDYLEDAFKTFEKRLDEFESLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=481997 ICI50_RS09250 WP_188891590.1 1885479..1887275(+) (pepF) [Lactobacillus kefiranofaciens strain 1207]
ATGGCAATACCAACTAGAGATGAAGTCCCAGCAGAACTTAAATGGGACTTGACGAGAATTTTTAAGACAGATCAAGATTG
GGAGCAGGCTTACGACGACACTAAACAAGAAGTAGCTGAACTTGATCAATTAAAAGGATCATTAGCTAAGTCGGGACAAG
ATTTATACGAAGGCTTAACCAAGATCTTAGCAGTTAGACGCAAGGTAGAAAATATCTACGTTTATGCCACTATGTCTAGT
GATGTCGATACCGCTAATTCACACTACTTAGGCTATGTTAGCCGGGTGCAGAGTTTGGCTAATCAATTCGAAGCCGTAAC
CAGCTTTATTAGTCCAGAAATCTTGAGCATCCCAGAAGACAAGTTCAAGCAATTTTTGCAAGCTGAACCACGCTTAAAGA
ATTACGCTCACTACTTGGAAACGATCACTAATAAGCGGCCCCATACTTTACCAGCTGAGGAAGAAAAAATTATTGCCGAT
GCCGGGGATGCCTTAAGCACTTCAGAGAACACTTTCAATGTTTTAACCAACTCCGACATGGAATACGGCTACGTGCAAGA
TGACGACGGCAATATGGAACAGTTGTCTGATGGGCTTTATTCATTATTGATTCAGTCACAGAACCGTGACGTCAGAAAAG
GTGCCTTTGACACGCTCTATACAACATATGGTCAATTTCAAAACTCCTTAGCTTCTACTCTTTCCGGCGTAGTCAAGAAG
CACAACTATAACGCTCGCGTGCACAAGTATAATTCGGCACGTGAAGCCGCATTAGCTGAAAACGGCGTACCAACCAAGGT
TTACGACACTTTGATTCAAGAAGTTGATTCACACCTTGACCTGCTTCACCGTTATGTTGCGCTGCGTAAGAAAATTTTAG
GCTTGAAAGATTTACAAATGTGGGACATGTACGTGCCGCTAACGGGTAAGCCATCACTGTCCTACAATTTTAAGGAAGCA
AAAGAAGTTGCCAAGAAGGCCTTGGCCCCACTTGGTGAAGACTACTTGAAGCATGTCGATTATATTTTTAATAACCGCGT
GATCGACAGCGTGGAAAATAAAAACAAGGTGACTGGTGCTTACTCTGGTGGTGCTTACGGCACTGACCCATATGAACTGC
TCAACTGGGAAGACAATATTGATTCACTTTACACTTTAGTTCACGAAACCGGTCACTCCGTTCACTCTTGGTATACGCGT
CATACCCAGCCTTACATCTATGGCGAGTATCCGATCTTCGTGGCTGAGATTGCTTCAACGACGAACGAAAATATTTTGAC
CGAATATTTCCTGGGGCATATTACCGATCCGAAGACGCGGGCCTTTATTCTGAACTACTACCTTGACTCATTCAAGGGCA
CGCTGTTCCGTCAAACGCAATTCGCTGTTTTTGAACAATTCTTGCATGAAGCTGATGCCAAAGGCGAACCATTAACTGCC
GATACTCTGGATGAGGTTTATGGACAAATCAACCAGCACTACTACGGTGATGCGGTTGAACCCGGCGGCGATATTGCCTT
AGAGTGGTCACGAATTCCGCACTTCTACTACAACTTCTACGTTTACCAATATGCGACGGGCTTTGCGGCTGCGACTGCTT
TGGCTAACAAGGTAGTACATGGAACAACGGCGGAGAGGGATGCCTACCTTGGCTTCCTTAAGGCTGGATCTAGCGATTAC
CCAACAGAGATCATGAAGCACGCTGGCGTGGACATGACTAAGCCGGATTACTTGGAAGATGCATTTAAGACTTTTGAAAA
GCGCTTGGATGAATTTGAGAGTTTGATTGAGAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-583)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.422

99.164

0.5