Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ICI50_RS01945 Genome accession   NZ_CP061341
Coordinates   387795..389171 (+) Length   458 a.a.
NCBI ID   WP_188891845.1    Uniprot ID   -
Organism   Lactobacillus kefiranofaciens strain 1207     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 382795..394171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICI50_RS10740 - 383807..384190 (-) 384 Protein_372 hypothetical protein -
  ICI50_RS01925 (ICI50_01920) - 384397..385239 (-) 843 WP_013853539.1 RhaT/GlcU family sugar-proton symporter -
  ICI50_RS01930 (ICI50_01925) pepC 385383..386732 (-) 1350 WP_013853540.1 aminopeptidase C -
  ICI50_RS01935 (ICI50_01930) - 386796..387110 (-) 315 WP_013853541.1 hypothetical protein -
  ICI50_RS01940 (ICI50_01935) - 387244..387795 (+) 552 WP_056940889.1 dUTP diphosphatase -
  ICI50_RS01945 (ICI50_01940) radA 387795..389171 (+) 1377 WP_188891845.1 DNA repair protein RadA Machinery gene
  ICI50_RS01950 (ICI50_01945) gltX 389248..390747 (+) 1500 WP_188891847.1 glutamate--tRNA ligase -
  ICI50_RS01955 (ICI50_01950) cysS 390843..392276 (+) 1434 WP_013853545.1 cysteine--tRNA ligase -
  ICI50_RS01960 (ICI50_01955) - 392269..392712 (+) 444 WP_013853546.1 ribonuclease III domain-containing protein -
  ICI50_RS01965 (ICI50_01960) rlmB 392699..393451 (+) 753 WP_188891849.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ICI50_RS01970 (ICI50_01965) - 393592..394140 (+) 549 WP_013853548.1 DNA-directed RNA polymerase subunit sigma -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50171.76 Da        Isoelectric Point: 7.0110

>NTDB_id=481986 ICI50_RS01945 WP_188891845.1 387795..389171(+) (radA) [Lactobacillus kefiranofaciens strain 1207]
MARVKTQYKCHSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIKKTGINEPVKLDKVKAEKEDRIHTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAITMAVASSYTDKEISPIDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRVFIPRHNMHASLNSLGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=481986 ICI50_RS01945 WP_188891845.1 387795..389171(+) (radA) [Lactobacillus kefiranofaciens strain 1207]
ATGGCACGAGTAAAAACACAATATAAATGTCATTCTTGTGGCTATATTTCCGCCAGCTATTTGGGACGTTGTCCTAATTG
TGGCGCGTGGAATCAGTTTGAAAAAGAAACTGAAGAAGTACAAAAGCGTTCTACAAAAGCGACTGCTAGCCGTTTAATCA
AAAAGACGGGTATCAATGAACCAGTTAAATTAGACAAGGTTAAGGCAGAAAAAGAAGACCGCATCCATACTAAGTCAGAG
GAGTTAAATCGCGTCTTGGGTGGTGGCATCGTCCCAGGTTCGCTTGTTTTAATTGGCGGGGATCCTGGTATTGGCAAATC
GACCTTGATGTTGCAGATCATGAGCGACTTATCGGAAAAATATAAGGTGCTCTACGTTTCTGGGGAAGAATCAGCTAATC
AGATTAAATTAAGAGCCGATCGTTTAGGCGTGGGTCAAAGTAATATGTTGCTATATCCTGAAACAGACATGCATGATATT
CGCGAACAAATCAACGATGTAAAGCCTGATTTCGTAGTCATCGATTCGATTCAAACCATGAATGAGCCTAGTCTTGATTC
AATGACTGGTTCAGCTTCGCAAGTGCGTGAAGTGACCAGCGAACTCATGAAGATCGCTAAGATGGACGCCATTACCGTGT
TTGTCATTGGTCACGTGACTAAGGAAGGCGCGATTGCAGGACCGAAGATTTTGGAGCACATGGTGGACACGGTCCTCTAT
TTTGAGGGGGATGAACACCATTCTTACCGTATTTTGCATTCAGTGAAAAACCGTTTTGGTGCGGCTAACGAAATTGGTAT
GTTTGAGATGGAGAATGAAGGGCTAAAAGAAGTAACCAACCCATCATCGATTTTTCTTGATCAAAGATTACCCAACTCTA
CTGGTTCAGCTGTGGTAGTTTCTCTAGAAGGAACTAGGCCGCTGCTTGCAGAAATTCAAGCATTAGTAACGCCAACTGCT
TTTGGCTATGCTAAAAGAACGACTTCAGGCATTAGTTTCAATAAGGCTTCATTATTATTGGCCGTGCTGGAAAAACGAGG
CAATTTAATGTTGCAAAACCAGGATGTTTATTTGACTGCGACTGGCGGCATTAAATTAAATGAACCGGCGATTGATTTGG
CTATTACAATGGCAGTAGCTTCAAGCTACACTGATAAAGAAATTTCGCCTATAGATTGTTTTGTAGGTGAAGTAGGGCTA
ACCGGCGAAGTTCGTCGCGTTGATAAAATTGAAGCACGAGTAAAAGAAGCCGCTAAAGTGGGCTTCAAGCGTGTTTTTAT
TCCGCGGCATAATATGCATGCTAGTTTAAACAGCTTAGGCATTGAGGTTATTCCGGTTTCCAGCATTCCACAGGCGTTAA
AATTAGTTTTTGGTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.338

100

0.555