Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EHE19_RS10520 Genome accession   NZ_CP061336
Coordinates   2562477..2563385 (-) Length   302 a.a.
NCBI ID   WP_137695975.1    Uniprot ID   A0A4U7JMF6
Organism   Ruminiclostridium herbifermentans strain MA18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557477..2568385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHE19_RS10505 (EHE19_010505) - 2558511..2558672 (+) 162 WP_137695978.1 DUF1540 domain-containing protein -
  EHE19_RS10510 (EHE19_010510) - 2558997..2559218 (-) 222 WP_137695977.1 PC4/YdbC family ssDNA-binding protein -
  EHE19_RS10515 (EHE19_010515) - 2559274..2562186 (-) 2913 WP_137695976.1 UPF0182 family protein -
  EHE19_RS10520 (EHE19_010520) pilA 2562477..2563385 (-) 909 WP_137695975.1 signal recognition particle-docking protein FtsY Machinery gene
  EHE19_RS10525 (EHE19_010525) smc 2563501..2567073 (-) 3573 WP_137695974.1 chromosome segregation protein SMC -
  EHE19_RS10530 (EHE19_010530) - 2567428..2568183 (-) 756 WP_137695973.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 302 a.a.        Molecular weight: 33008.26 Da        Isoelectric Point: 5.0656

>NTDB_id=481926 EHE19_RS10520 WP_137695975.1 2562477..2563385(-) (pilA) [Ruminiclostridium herbifermentans strain MA18]
MGFFDKLKDGLMKTRKSITEKIDQVLVSFGKVDEELFDELEEILITSDIGIDTTMKIIASLKEKVRERKIIDAMKVKDLL
KEELKEILESGDNSLKLETKPSVIIVIGVNGVGKTTSIGKIANYYKNQGKKVLLAAGDTFRAAAIDQLEIWADRVGVDII
GQKEGSDPAAVVFDAAQAAKSRKADLLICDTAGRLHTKKNLMEELKKVSRILDRELPGADRETLLVLDATTGQNAISQAK
TFRETADITGIVLTKLDGTAKGGIVVAIKSELDIPVKLIGVGEQMDDLQKFNASEFVEALFS

Nucleotide


Download         Length: 909 bp        

>NTDB_id=481926 EHE19_RS10520 WP_137695975.1 2562477..2563385(-) (pilA) [Ruminiclostridium herbifermentans strain MA18]
ATGGGTTTTTTTGATAAGTTAAAAGATGGATTAATGAAAACAAGGAAAAGTATCACAGAAAAGATAGATCAAGTACTTGT
TTCATTTGGAAAGGTTGATGAGGAATTATTTGATGAACTTGAGGAAATTCTAATAACATCTGATATTGGTATTGATACCA
CAATGAAGATAATTGCAAGCTTAAAGGAAAAGGTTCGTGAAAGAAAAATTATTGATGCAATGAAGGTAAAGGATTTATTG
AAGGAAGAACTTAAGGAGATACTAGAGTCAGGAGATAATAGCCTAAAATTAGAAACTAAGCCTTCAGTTATTATAGTAAT
TGGTGTAAATGGAGTAGGAAAGACTACTTCAATAGGCAAGATTGCAAACTATTATAAAAATCAAGGGAAAAAAGTACTGC
TTGCAGCAGGTGATACCTTTAGAGCTGCAGCAATAGATCAGTTAGAAATATGGGCAGATAGAGTTGGTGTAGATATTATT
GGGCAGAAGGAAGGCTCAGACCCAGCAGCTGTAGTATTTGATGCTGCACAGGCTGCAAAGTCTAGAAAAGCTGATTTACT
TATTTGTGATACAGCAGGAAGATTGCATACAAAGAAAAATCTTATGGAAGAACTAAAAAAGGTTTCAAGAATTTTGGATA
GAGAGTTACCAGGAGCAGATAGAGAAACATTGCTGGTACTAGATGCAACTACTGGACAGAACGCTATTTCACAAGCAAAA
ACTTTTAGAGAAACAGCTGATATTACAGGTATAGTACTTACTAAGCTGGATGGTACAGCAAAAGGCGGAATTGTAGTTGC
AATAAAATCAGAACTTGATATACCTGTAAAGCTAATTGGTGTGGGAGAGCAGATGGACGATTTGCAAAAATTCAATGCGT
CTGAGTTTGTAGAAGCATTATTTTCATAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(101-301)

SRP54-type protein, helical bundle domain (SRP54_N)

(19-84)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U7JMF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.185

100

0.483