Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IC571_RS07695 Genome accession   NZ_CP061321
Coordinates   1476237..1477601 (-) Length   454 a.a.
NCBI ID   WP_215315759.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH2A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1471237..1482601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC571_RS07680 (IC571_07670) dapD 1473288..1474115 (+) 828 WP_215315755.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  IC571_RS07685 (IC571_07675) dapE 1474137..1475288 (+) 1152 WP_215315756.1 succinyl-diaminopimelate desuccinylase -
  IC571_RS07690 (IC571_07680) prmB 1475329..1476225 (+) 897 WP_215315758.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  IC571_RS07695 (IC571_07685) radA 1476237..1477601 (-) 1365 WP_215315759.1 DNA repair protein RadA Machinery gene
  IC571_RS07700 (IC571_07690) - 1477638..1478126 (-) 489 WP_215315760.1 hypothetical protein -
  IC571_RS07705 (IC571_07695) - 1478144..1479985 (-) 1842 WP_251373567.1 potassium transporter Kup -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48212.04 Da        Isoelectric Point: 7.3475

>NTDB_id=481816 IC571_RS07695 WP_215315759.1 1476237..1477601(-) (radA) [Polynucleobacter sp. MWH-UH2A]
MAKVKTVFICQSCGGTSAKWQGQCPSCQSWNTMEEGLPESTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSAAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKASGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLIRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKAKIMGLRAIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481816 IC571_RS07695 WP_215315759.1 1476237..1477601(-) (radA) [Polynucleobacter sp. MWH-UH2A]
TTGGCTAAAGTAAAAACAGTCTTCATCTGTCAGTCTTGCGGCGGCACTTCGGCGAAGTGGCAGGGGCAATGTCCATCATG
CCAATCCTGGAATACGATGGAAGAGGGTTTGCCAGAGTCAACTTCAAATTCTCGGTTTCAGGGCTTAGCACAATCTTTAC
CGAGACAAAAACTTTCTGCCATTACAGCGGAAGATTTACCAAGATTCAGCACTGGTGTTGAGGAGTTTGATCGTGTGTTG
GGTGGTGGACTGGTTCCTGGTGGCGTTGTATTGCTGGGCGGTGATCCTGGCATCGGTAAATCTACTTTGCTATTGCAAGC
CCTTGCAGAGATGAGTGCTGCTGGCATGAATGTTCTTTACAGTAGTGGCGAAGAGTCTGCCGCACAAATTGCCTTGCGCG
CAAAGCGAATCGCCTTAGATGCGCCGCAATTGGAAGTATTGGCAGAGATTCAGTTGGAGAAACTGATTTCTATTATGGAT
ACCGTTAAACCACAAGTATTGGTGGTGGACTCCATTCAAACTTTGTACTCAGAGGTATTGAGCTCGGCACCAGGATCGGT
GGCGCAGGTAAGAGAGTGTGCGGCTCAGTTAACGAGAGCAGCTAAAGCGAGTGGTATTTGCGTGCTGATGGTAGGTCATG
TCACCAAAGATGGTCATTTAGCTGGCCCACGCGTACTTGAACACATTGTGGATACAGTGTTGTACTTTGAAGGCGATACC
CATTCCTCTTTTCGTTTAATTCGCTCTATCAAGAATCGCTTTGGCGCAGTTAATGAACTGGGTGTATTTGCAATGACTGA
GAAAGGGCTGCGTGGAGTGACAAACCCATCAGCGATTTTCTTATCACAACATGAGCAAATGGTTCCTGGTGCATGTGTGC
TGGTGACGCAAGAAGGCAGTAGACCGTTGCTGGTCGAAATTCAGGCGCTGGTGGATACTGCACATGTGCCTAACCCTCGT
CGCTTGGCTGTGGGTTTGGAGCAGGCGCGCTTAGCAATGTTATTGGCGGTCTTACACCGTCATGCAGGTGTGGCTTGTTT
TGATCAAGACGTATTTCTGAATGCGGTAGGTGGTGTAAAAATTTCTGAGCCAGCCGCTGACTTAGCAGTCCTTTTGGCGA
TTCAATCATCTATTCGCAATCGCGCATTACCAAAAGAATTAATTGTGTTTGGCGAAGTCGGTTTAGCGGGAGAAATTCGT
CCATGTCCGCGTGGGCAAGAGCGTCTCAAGGAAGCGGCAAAGTTGGGATTTACGGTTGCCATCATTCCGAAAGCCAATAT
GCCAAAGGCAAAAATTATGGGCTTGAGAGCGATTCCTGTGGAGCGAATTGATCAAGCGATCGCTGCAGCTGCAGAATTAA
GTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(78-218)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.115

99.559

0.489
  radA Streptococcus pneumoniae Rx1

47.319

94.493

0.447
  radA Streptococcus pneumoniae D39

47.319

94.493

0.447
  radA Streptococcus pneumoniae R6

47.319

94.493

0.447
  radA Streptococcus pneumoniae TIGR4

47.319

94.493

0.447
  radA Streptococcus mitis NCTC 12261

47.319

94.493

0.447
  radA Streptococcus mitis SK321

47.319

94.493

0.447