Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GQ359_RS09355 Genome accession   NZ_CP061319
Coordinates   1797670..1798560 (+) Length   296 a.a.
NCBI ID   WP_215386894.1    Uniprot ID   -
Organism   Polynucleobacter sp. AM-7D1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1792670..1803560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ359_RS09325 (GQ359_09330) pth 1792848..1793444 (+) 597 WP_215386889.1 aminoacyl-tRNA hydrolase -
  GQ359_RS09330 (GQ359_09335) - 1793449..1793718 (-) 270 WP_215386890.1 YfhL family 4Fe-4S dicluster ferredoxin -
  GQ359_RS09335 (GQ359_09340) coaD 1793736..1794230 (-) 495 WP_215386891.1 pantetheine-phosphate adenylyltransferase -
  GQ359_RS09340 (GQ359_09345) rsmD 1794267..1794896 (-) 630 WP_215387959.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GQ359_RS09345 (GQ359_09350) - 1794889..1796235 (-) 1347 WP_215386892.1 pitrilysin family protein -
  GQ359_RS09350 (GQ359_09355) - 1796222..1797607 (-) 1386 WP_215386893.1 pitrilysin family protein -
  GQ359_RS09355 (GQ359_09360) pilA 1797670..1798560 (+) 891 WP_215386894.1 signal recognition particle-docking protein FtsY Machinery gene
  GQ359_RS09360 (GQ359_09365) rpoH 1798655..1799596 (+) 942 WP_215386895.1 RNA polymerase sigma factor RpoH -
  GQ359_RS09365 (GQ359_09370) - 1799619..1800224 (-) 606 WP_215386896.1 SCO family protein -
  GQ359_RS09370 (GQ359_09375) cyoE 1800232..1801125 (-) 894 WP_215386897.1 heme o synthase -
  GQ359_RS09375 (GQ359_09380) - 1801122..1802252 (-) 1131 WP_215386898.1 heme A synthase -
  GQ359_RS09380 (GQ359_09385) - 1802249..1802908 (-) 660 WP_215386899.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31461.23 Da        Isoelectric Point: 7.4791

>NTDB_id=481810 GQ359_RS09355 WP_215386894.1 1797670..1798560(+) (pilA) [Polynucleobacter sp. AM-7D1]
MFGLRKTLGSLFKSNQTDEAWFDALEESLILSDVGLPTTEQLMSKLRKAAKSENASSPEDLKQLLIQEVASLLKPLEPIT
NPLFVHEQKTTPEIWLVIGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVIMQESGDAAA
VAHDAIHAAVSRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHQTLLVLDGNTGQNGLSQVKAFHAALGLSA
LIVTKLDGTAKGGVICALAHTLKDGSKPAVLALGKGEGINDLAPFTAAQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481810 GQ359_RS09355 WP_215386894.1 1797670..1798560(+) (pilA) [Polynucleobacter sp. AM-7D1]
ATGTTCGGCTTACGTAAAACCCTCGGATCCCTGTTTAAATCGAATCAGACTGATGAAGCCTGGTTTGATGCCCTAGAAGA
ATCTCTCATTTTGAGTGATGTGGGTCTACCTACGACCGAACAACTGATGAGCAAACTTCGCAAGGCTGCCAAATCAGAAA
ATGCCAGCAGTCCAGAAGACCTAAAGCAACTGCTGATTCAAGAGGTGGCAAGTCTCCTAAAGCCTTTGGAGCCAATCACT
AACCCTTTATTTGTTCATGAACAAAAGACCACTCCGGAAATATGGCTAGTAATTGGCGTCAATGGCGCTGGTAAGACCAC
CACCATCGGCAAACTCTGCAAACTCTTTCAGTCACAAGGCAAGTCCGTCTTACTGGCAGCAGGTGATACTTTCCGGGCGG
CTGCACGCAATCAGCTCCTGGAATGGGGTGGGCGCAACCAAGTCGACGTCATCATGCAAGAAAGTGGTGATGCTGCAGCC
GTAGCGCATGATGCGATTCATGCCGCAGTGTCTCGTAAGAGCGATATTTTGATTATTGATACCGCGGGTAGATTAGCCAC
TCAAGACCATCTCATGGAAGAATTGAAGAAGGTTAAAAGAGTCATTGGCAAGGCCCTTCCTGGGGCCCCTCACCAGACTT
TGCTGGTTCTTGATGGCAATACTGGTCAAAATGGTTTAAGCCAAGTAAAGGCCTTTCATGCTGCTTTAGGCCTATCAGCC
TTAATCGTGACAAAGTTAGATGGCACCGCTAAAGGTGGAGTGATCTGTGCGCTTGCTCACACCCTCAAAGATGGGTCAAA
ACCAGCGGTTTTAGCCCTAGGCAAAGGTGAGGGAATTAACGATTTAGCCCCTTTCACTGCCGCGCAATATTCTTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(93-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.242

98.986

0.527