Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FD971_RS09125 Genome accession   NZ_CP061318
Coordinates   1770148..1771038 (+) Length   296 a.a.
NCBI ID   WP_215333998.1    Uniprot ID   -
Organism   Polynucleobacter sp. AP-Ainpum-60-G11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1765148..1776038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD971_RS09095 (FD971_09090) pth 1765325..1765921 (+) 597 WP_215333993.1 aminoacyl-tRNA hydrolase -
  FD971_RS09100 (FD971_09095) - 1765926..1766195 (-) 270 WP_215333994.1 YfhL family 4Fe-4S dicluster ferredoxin -
  FD971_RS09105 (FD971_09100) coaD 1766214..1766708 (-) 495 WP_215333995.1 pantetheine-phosphate adenylyltransferase -
  FD971_RS09110 (FD971_09105) rsmD 1766745..1767374 (-) 630 WP_215335325.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  FD971_RS09115 (FD971_09110) - 1767367..1768713 (-) 1347 WP_215333996.1 pitrilysin family protein -
  FD971_RS09120 (FD971_09115) - 1768700..1770085 (-) 1386 WP_215333997.1 pitrilysin family protein -
  FD971_RS09125 (FD971_09120) pilA 1770148..1771038 (+) 891 WP_215333998.1 signal recognition particle-docking protein FtsY Machinery gene
  FD971_RS09130 (FD971_09125) rpoH 1771132..1772073 (+) 942 WP_215333999.1 RNA polymerase sigma factor RpoH -
  FD971_RS09135 (FD971_09130) - 1772092..1772655 (-) 564 WP_371743081.1 SCO family protein -
  FD971_RS09140 (FD971_09135) cyoE 1772705..1773598 (-) 894 WP_215334001.1 heme o synthase -
  FD971_RS09145 (FD971_09140) - 1773595..1774719 (-) 1125 WP_251368620.1 heme A synthase -
  FD971_RS09150 (FD971_09145) - 1774716..1775375 (-) 660 WP_215334002.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31691.50 Da        Isoelectric Point: 6.9157

>NTDB_id=481799 FD971_RS09125 WP_215333998.1 1770148..1771038(+) (pilA) [Polynucleobacter sp. AP-Ainpum-60-G11]
MFGLRKTLGSLFKSNQTDEAWFDTLEESLILSDVGLPTTEQLISKLRKAAKSENINSPEELKQLLIQEVATLLRPLEPNP
NPLYVHEQKTMPEVWLVIGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVIMQESGDAAA
VAHDAIHAAISRKSDILIIDTAGRLATQEHLMEELKKVKRVIGKALPGAPHQTLLVLDGNTGQNGLSQVKAFHGALGLSA
LIVTKLDGTAKGGVICALAHTLKEGHIPAVLALGKGEGIDDLAPFTAAQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481799 FD971_RS09125 WP_215333998.1 1770148..1771038(+) (pilA) [Polynucleobacter sp. AP-Ainpum-60-G11]
ATGTTCGGCCTACGTAAAACCCTCGGATCCCTATTCAAATCCAATCAGACTGATGAGGCCTGGTTTGATACCTTAGAAGA
ATCGCTCATTCTGAGTGACGTGGGCCTTCCTACGACTGAACAATTAATCAGCAAACTTCGTAAAGCTGCTAAATCAGAAA
ATATCAATAGCCCGGAAGAGCTGAAACAATTACTAATCCAAGAGGTTGCGACCCTTCTAAGGCCTTTGGAACCAAACCCC
AATCCTTTGTATGTGCATGAGCAAAAGACCATGCCAGAGGTGTGGCTAGTTATCGGCGTGAATGGTGCAGGGAAGACCAC
CACCATCGGTAAGCTGTGCAAACTCTTTCAGTCCCAAGGTAAATCTGTCTTACTGGCGGCAGGCGATACTTTTCGAGCAG
CAGCCCGTAACCAGCTCCTGGAATGGGGTGGCCGCAATCAAGTCGACGTCATCATGCAAGAGAGTGGTGATGCTGCGGCG
GTGGCACATGATGCTATTCATGCGGCAATCTCGCGTAAGAGCGATATTTTGATTATTGATACCGCAGGTAGGTTGGCCAC
GCAAGAACACCTGATGGAAGAATTGAAGAAGGTGAAAAGGGTGATCGGCAAGGCCCTTCCTGGGGCGCCTCACCAGACTT
TGCTCGTTCTTGATGGCAATACTGGTCAAAACGGTTTAAGCCAAGTTAAGGCCTTTCATGGGGCTTTAGGGCTCTCAGCC
TTAATCGTGACTAAATTAGATGGCACGGCAAAAGGTGGGGTTATCTGTGCACTCGCCCACACCCTCAAAGAGGGGCATAT
TCCAGCGGTTTTAGCTTTGGGCAAAGGTGAAGGAATTGATGATTTAGCCCCTTTCACTGCCGCCCAATATTCTTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.242

98.986

0.527