Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FD975_RS09455 Genome accession   NZ_CP061316
Coordinates   1821347..1822237 (+) Length   296 a.a.
NCBI ID   WP_215302136.1    Uniprot ID   -
Organism   Polynucleobacter sp. AP-Jannik-300A-C4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1816347..1827237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD975_RS09425 (FD975_09420) pth 1816505..1817101 (+) 597 WP_215302131.1 aminoacyl-tRNA hydrolase -
  FD975_RS09430 (FD975_09425) - 1817129..1817395 (-) 267 WP_215302132.1 YfhL family 4Fe-4S dicluster ferredoxin -
  FD975_RS09435 (FD975_09430) coaD 1817413..1817907 (-) 495 WP_215302133.1 pantetheine-phosphate adenylyltransferase -
  FD975_RS09440 (FD975_09435) rsmD 1817944..1818573 (-) 630 WP_215303981.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  FD975_RS09445 (FD975_09440) - 1818566..1819912 (-) 1347 WP_215302134.1 pitrilysin family protein -
  FD975_RS09450 (FD975_09445) - 1819899..1821284 (-) 1386 WP_215302135.1 pitrilysin family protein -
  FD975_RS09455 (FD975_09450) pilA 1821347..1822237 (+) 891 WP_215302136.1 signal recognition particle-docking protein FtsY Machinery gene
  FD975_RS09460 (FD975_09455) rpoH 1822332..1823273 (+) 942 WP_215302137.1 RNA polymerase sigma factor RpoH -
  FD975_RS09465 (FD975_09460) - 1823296..1823901 (-) 606 WP_215302138.1 SCO family protein -
  FD975_RS09470 (FD975_09465) cyoE 1823909..1824802 (-) 894 WP_215302139.1 heme o synthase -
  FD975_RS09475 (FD975_09470) - 1824799..1825929 (-) 1131 WP_215302140.1 heme A synthase -
  FD975_RS09480 (FD975_09475) - 1825926..1826585 (-) 660 WP_215302141.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31413.13 Da        Isoelectric Point: 7.4791

>NTDB_id=481788 FD975_RS09455 WP_215302136.1 1821347..1822237(+) (pilA) [Polynucleobacter sp. AP-Jannik-300A-C4]
MFGLRKTLGSLFKSNQTDEAWFDALEESLILSDVGLPTTEQLISKLRKAAKSENASSPEDLKQLLIQEVASLLKPLEPSP
NPLFVHEQKTTPEIWLVIGVNGAGKTTTIGKICKLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVIMQESGDAAA
VAHDAIHAAVSRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHQTLLVLDGNTGQNGLSQVKAFHAALGLSA
LIVTKLDGTAKGGVICALAHTLKDGSKPAVLALGKGEGINDLAPFTAAQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481788 FD975_RS09455 WP_215302136.1 1821347..1822237(+) (pilA) [Polynucleobacter sp. AP-Jannik-300A-C4]
ATGTTCGGCTTACGTAAAACCCTCGGATCCCTATTCAAATCGAATCAGACTGACGAAGCCTGGTTTGATGCCCTAGAAGA
ATCTCTCATTTTGAGTGATGTAGGCCTACCTACGACCGAACAACTGATTAGCAAACTTCGCAAGGCTGCCAAATCAGAAA
ATGCCAGTAGTCCAGAAGACCTAAAGCAACTACTGATTCAAGAGGTGGCGAGTCTCCTAAAGCCTTTGGAGCCAAGCCCT
AACCCTTTATTTGTTCATGAACAAAAGACCACTCCGGAAATATGGCTAGTAATTGGCGTCAATGGTGCTGGTAAGACCAC
CACCATCGGCAAAATCTGCAAACTCTTTCAGTCACAAGGCAAGTCCGTCTTACTGGCAGCAGGCGATACTTTCCGAGCGG
CTGCACGCAATCAGCTCCTGGAATGGGGTGGGCGCAACCAAGTCGACGTCATCATGCAAGAAAGTGGTGATGCTGCAGCC
GTAGCGCATGACGCGATTCATGCTGCAGTATCTCGTAAGAGCGACATTTTGATTATTGATACCGCGGGTAGATTAGCCAC
TCAAGACCATCTCATGGAAGAATTGAAGAAGGTTAAAAGAGTCATTGGCAAGGCCCTTCCTGGGGCCCCTCACCAGACTT
TGCTCGTTCTTGATGGCAATACCGGTCAAAATGGTTTAAGCCAAGTAAAGGCCTTTCATGCTGCTTTAGGCCTATCAGCC
TTAATCGTGACAAAGTTAGATGGCACCGCTAAAGGTGGAGTGATCTGTGCGCTTGCTCACACCCTCAAAGATGGGTCAAA
ACCAGCGGTTTTAGCCCTAGGCAAAGGTGAGGGAATTAACGATTTAGCCCCTTTCACTGCCGCACAATATTCTTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(93-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.56

98.986

0.52