Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C2745_RS08915 Genome accession   NZ_CP061315
Coordinates   1790340..1791230 (+) Length   296 a.a.
NCBI ID   WP_215384244.1    Uniprot ID   -
Organism   Polynucleobacter sp. AP-Kolm-20A-A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1785340..1796230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2745_RS08885 (C2745_08885) pth 1785537..1786127 (+) 591 WP_215384239.1 aminoacyl-tRNA hydrolase -
  C2745_RS08890 (C2745_08890) - 1786145..1786411 (-) 267 WP_215384240.1 YfhL family 4Fe-4S dicluster ferredoxin -
  C2745_RS08895 (C2745_08895) coaD 1786428..1786925 (-) 498 WP_215384241.1 pantetheine-phosphate adenylyltransferase -
  C2745_RS08900 (C2745_08900) rsmD 1786962..1787600 (-) 639 WP_251368322.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  C2745_RS08905 (C2745_08905) - 1787572..1788909 (-) 1338 WP_215384242.1 pitrilysin family protein -
  C2745_RS08910 (C2745_08910) - 1788909..1790276 (-) 1368 WP_215384243.1 pitrilysin family protein -
  C2745_RS08915 (C2745_08915) pilA 1790340..1791230 (+) 891 WP_215384244.1 signal recognition particle-docking protein FtsY Machinery gene
  C2745_RS08920 (C2745_08920) rpoH 1791324..1792247 (+) 924 WP_215384245.1 RNA polymerase sigma factor RpoH -
  C2745_RS08925 (C2745_08925) - 1792266..1792850 (-) 585 WP_215384246.1 SCO family protein -
  C2745_RS08930 (C2745_08930) cyoE 1792857..1793750 (-) 894 WP_215384247.1 heme o synthase -
  C2745_RS08935 (C2745_08935) - 1793753..1794874 (-) 1122 WP_215384248.1 heme A synthase -
  C2745_RS08940 (C2745_08940) - 1794874..1795530 (-) 657 WP_215384249.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31383.00 Da        Isoelectric Point: 7.4790

>NTDB_id=481777 C2745_RS08915 WP_215384244.1 1790340..1791230(+) (pilA) [Polynucleobacter sp. AP-Kolm-20A-A1]
MFGLRKTLGSLFKSSKVDEAWFDHLEESLIQSDVGLPTTEQLINKLRKAAKSEKASSPEELQALLIQEVSSLLAAIEPSP
NPLFISQKANTPEVWLVVGVNGAGKTTTIGKLCKLFQSQGKSILLAAGDTFRAAARNQLQEWGGRNQVDVITQDGGDAAA
VAHDAIHAAVSRKNDILIIDTAGRLATQDNLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQDGPKPAVLALGKGEKIDDLAPFTAGQYSFELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481777 C2745_RS08915 WP_215384244.1 1790340..1791230(+) (pilA) [Polynucleobacter sp. AP-Kolm-20A-A1]
ATGTTCGGCTTACGTAAAACCCTAGGATCCCTTTTTAAATCAAGCAAAGTTGATGAAGCTTGGTTTGATCATTTAGAAGA
ATCACTGATTCAAAGTGATGTGGGTCTACCTACGACTGAACAACTCATCAACAAGTTACGCAAAGCAGCAAAATCTGAAA
AAGCTTCAAGCCCTGAAGAGTTGCAAGCGCTCCTTATTCAAGAAGTAAGCTCACTTCTCGCAGCCATTGAGCCTTCGCCA
AACCCCCTCTTTATAAGCCAGAAAGCCAATACACCAGAGGTATGGTTGGTTGTAGGGGTAAACGGCGCAGGTAAGACAAC
AACGATAGGTAAGCTTTGCAAACTCTTTCAGTCTCAAGGTAAATCGATTCTCTTGGCCGCTGGCGATACCTTTAGAGCTG
CCGCCCGCAACCAATTGCAAGAATGGGGTGGTCGCAATCAGGTAGACGTTATCACCCAAGATGGTGGTGATGCCGCTGCA
GTTGCGCATGACGCTATTCATGCAGCGGTTTCTCGTAAAAATGACATTCTCATTATTGATACCGCAGGCCGTCTTGCCAC
GCAGGACAACCTGATGGAAGAGCTGAAGAAGGTAAAGCGCGTGATCGGCAAAGCACTCCCTGGTGCGCCACACCACACTC
TGCTTGTTTTGGATGGCAACACAGGGCAAAACGGCTTAAGCCAGGTCAAAGCCTTTCATGCTGCCCTGGGCCTAACTGGG
ATCATCGTTACCAAGCTGGATGGGACTGCCAAGGGCGGTGTTATCTGCGCCCTTGCCCACACCCTACAAGATGGTCCAAA
ACCCGCTGTGCTAGCCCTAGGAAAGGGCGAAAAAATTGATGATTTAGCCCCCTTTACAGCAGGGCAATATTCATTCGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.218

98.986

0.517