Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C2745_RS02615 Genome accession   NZ_CP061315
Coordinates   483981..485345 (+) Length   454 a.a.
NCBI ID   WP_215367268.1    Uniprot ID   -
Organism   Polynucleobacter sp. AP-Kolm-20A-A1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 478981..490345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2745_RS02595 (C2745_02595) - 480790..481161 (+) 372 WP_215384835.1 glycine zipper family protein -
  C2745_RS02605 (C2745_02605) - 481493..483436 (+) 1944 WP_215384836.1 potassium transporter Kup -
  C2745_RS02610 (C2745_02610) - 483481..483942 (+) 462 WP_251368363.1 hypothetical protein -
  C2745_RS02615 (C2745_02615) radA 483981..485345 (+) 1365 WP_215367268.1 DNA repair protein RadA Machinery gene
  C2745_RS02620 (C2745_02620) prmB 485362..486258 (-) 897 WP_215384838.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  C2745_RS02625 (C2745_02625) dapE 486305..487456 (-) 1152 WP_215384839.1 succinyl-diaminopimelate desuccinylase -
  C2745_RS02630 (C2745_02630) dapD 487466..488293 (-) 828 WP_128113278.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48240.03 Da        Isoelectric Point: 7.3470

>NTDB_id=481768 C2745_RS02615 WP_215367268.1 483981..485345(+) (radA) [Polynucleobacter sp. AP-Kolm-20A-A1]
MAKVKTVYICQSCGGTSAKWQGQCPSCQAWNTMEEGLPETTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLKVIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481768 C2745_RS02615 WP_215367268.1 483981..485345(+) (radA) [Polynucleobacter sp. AP-Kolm-20A-A1]
TTGGCTAAAGTCAAAACGGTTTATATCTGCCAATCTTGCGGTGGCACCTCTGCTAAGTGGCAAGGTCAATGCCCGTCATG
TCAGGCATGGAATACGATGGAAGAGGGTCTTCCAGAGACCACTTCTAATTCTCGTTTTCAGGGATTAGCACAATCGCTGC
CAAGGCAAAAGTTATCCGCTATTACAGCTGAAGACTTGCCAAGATTTAGCACTGGCGTAGAAGAGTTTGATCGTGTGCTC
GGTGGTGGCTTGGTTCCTGGCGGTGTAGTGCTTCTTGGTGGCGACCCAGGCATTGGTAAATCTACATTGCTATTGCAAGC
ATTGGCAGAGATGAGTTCTGCCGGCATGAATGTGCTTTATAGCAGCGGTGAAGAATCTGCTGCGCAGATTGCTTTGCGAG
CAAAACGTATTGCCTTGGACGCACCGCAGCTTGAAGTATTGGCGGAGATTCAGTTGGAGAAACTGATCTCTATTATGGAT
ACCGTCAAACCACAAGTCCTGGTGGTGGATTCGATCCAGACTTTATATTCAGAGGTATTGAGCTCTGCTCCTGGCTCTGT
AGCCCAGGTCCGTGAGTGTGCGGCGCAACTCACGCGAGCTGCTAAATCTAGTGGCATCTGTGTATTGATGGTGGGACACG
TGACTAAAGACGGCCATTTGGCTGGCCCAAGAGTATTGGAGCATATCGTCGACACCGTGCTGTATTTTGAGGGTGACACG
CATTCTTCTTTCCGCTTGGTGCGTTCCATTAAGAATCGCTTTGGCGCTGTTAATGAGTTGGGTGTATTTGCGATGACTGA
AAAAGGTCTGCGCGGCGTGACTAACCCGTCAGCGATCTTCTTGTCGCAACATGAGCAAATGGTTCCTGGTGCTTGCGTAT
TGGTAACGCAAGAAGGTAGCAGGCCTTTGTTGGTAGAGATTCAGGCTTTGGTGGATACAGCGCATGTCCCCAATCCACGC
CGTTTGGCTGTTGGCTTGGAGCAGGCTCGTCTAGCAATGCTGCTAGCAGTGCTGCATCGTCATGCTGGTGTTGCTTGTTT
TGATCAAGATGTCTTCTTAAATGCAGTTGGTGGCGTCAAGATTTCTGAGCCAGCTGCTGACTTAGCTGTCCTCTTGGCTA
TTCAGTCATCTATTCGTAATCGTGCGTTACCAAAAGAGTTGATCGTATTTGGTGAAGTAGGTTTGGCTGGGGAGATTCGC
CCTTGCCCACGGGGTCAGGAGCGCTTGAAAGAGGCTGCTAAATTAGGCTTTACTGTGGCGATTATTCCCAAAGCCAATAT
GCCCAAAACTAAGATCCCGGGATTAAAGGTTATCCCTGTTGAGCGGATTGATCAGGCGATTGCTGCCGCAGCAGAACTCA
GCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.558

99.559

0.493
  radA Streptococcus pneumoniae Rx1

45.778

99.119

0.454
  radA Streptococcus pneumoniae D39

45.778

99.119

0.454
  radA Streptococcus pneumoniae R6

45.778

99.119

0.454
  radA Streptococcus pneumoniae TIGR4

45.778

99.119

0.454
  radA Streptococcus mitis NCTC 12261

45.778

99.119

0.454
  radA Streptococcus mitis SK321

45.778

99.119

0.454