Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C2758_RS09925 Genome accession   NZ_CP061312
Coordinates   1897441..1898331 (+) Length   296 a.a.
NCBI ID   WP_215328107.1    Uniprot ID   -
Organism   Polynucleobacter sp. AP-Sving-400A-A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1892441..1903331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2758_RS09895 (C2758_09885) pth 1892612..1893208 (+) 597 WP_215328104.1 aminoacyl-tRNA hydrolase -
  C2758_RS09900 (C2758_09890) - 1893223..1893489 (-) 267 WP_215308512.1 YfhL family 4Fe-4S dicluster ferredoxin -
  C2758_RS09905 (C2758_09895) coaD 1893507..1894001 (-) 495 WP_215328105.1 pantetheine-phosphate adenylyltransferase -
  C2758_RS09910 (C2758_09900) rsmD 1894038..1894667 (-) 630 WP_371817721.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  C2758_RS09915 (C2758_09905) - 1894660..1896003 (-) 1344 WP_215330262.1 pitrilysin family protein -
  C2758_RS09920 (C2758_09910) - 1895993..1897378 (-) 1386 WP_215328106.1 pitrilysin family protein -
  C2758_RS09925 (C2758_09915) pilA 1897441..1898331 (+) 891 WP_215328107.1 signal recognition particle-docking protein FtsY Machinery gene
  C2758_RS09930 (C2758_09920) rpoH 1898425..1899366 (+) 942 WP_215328108.1 RNA polymerase sigma factor RpoH -
  C2758_RS09935 (C2758_09925) - 1899388..1899999 (-) 612 WP_215328109.1 SCO family protein -
  C2758_RS09940 (C2758_09930) cyoE 1900001..1900894 (-) 894 WP_215328111.1 heme o synthase -
  C2758_RS09945 (C2758_09935) - 1900891..1902021 (-) 1131 WP_215328112.1 heme A synthase -
  C2758_RS09950 (C2758_09940) - 1902018..1902677 (-) 660 WP_215328113.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31590.37 Da        Isoelectric Point: 6.2441

>NTDB_id=481754 C2758_RS09925 WP_215328107.1 1897441..1898331(+) (pilA) [Polynucleobacter sp. AP-Sving-400A-A2]
MFGLRKTLGSLFQSIQTDEAWFDALEESLILSDVGLLTTEQLINKLRKAAKSEKASSPEELKQLLIEEVAILLKALEANP
NPLYVHEQKTTPEVWLVIGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVIMQESGDAAA
VAHDAIHAAISRKSDILIIDTAGRLATQDHLMEELKKIKRVIGKALPGAPHQTLLVLDGNTGQNGLSQVRAFHAALELSA
LIVTKLDGTAKGGVICALAQTLGDGLKPAVLALGKGEGIDDLAPFTAAQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481754 C2758_RS09925 WP_215328107.1 1897441..1898331(+) (pilA) [Polynucleobacter sp. AP-Sving-400A-A2]
ATGTTCGGCCTACGTAAAACTCTCGGATCCTTATTCCAATCTATTCAGACTGATGAAGCCTGGTTTGATGCCCTAGAAGA
ATCTCTTATTCTGAGCGATGTGGGCCTTCTCACGACCGAACAATTGATTAATAAGCTCCGTAAAGCCGCCAAATCGGAAA
AAGCCAGCAGCCCTGAAGAACTCAAGCAACTACTGATCGAAGAAGTGGCGATACTCCTGAAAGCCTTGGAGGCTAACCCC
AATCCCCTGTACGTCCATGAGCAAAAGACCACACCAGAGGTGTGGCTAGTAATAGGTGTCAATGGTGCTGGCAAGACCAC
CACGATCGGTAAACTCTGCAGACTTTTTCAGTCGCAAGGTAAATCTGTCTTACTGGCAGCTGGCGATACTTTCCGGGCCG
CGGCACGCAACCAGCTCCTTGAATGGGGTGGCCGCAATCAGGTCGATGTCATCATGCAAGAAAGTGGTGATGCGGCGGCA
GTGGCGCACGATGCCATTCATGCGGCGATTTCTCGCAAAAGCGATATCCTGATCATTGATACCGCTGGCAGACTGGCTAC
TCAAGACCATCTGATGGAAGAGTTAAAGAAGATCAAAAGAGTGATTGGTAAAGCTCTTCCTGGGGCGCCTCACCAGACTT
TGCTTGTTCTTGATGGCAATACTGGTCAAAACGGTTTAAGCCAAGTAAGAGCCTTTCATGCCGCTTTAGAACTTTCTGCC
TTAATTGTGACTAAATTGGACGGCACCGCTAAAGGTGGAGTCATTTGCGCACTTGCACAGACCCTCGGCGATGGGCTAAA
ACCAGCGGTTCTAGCTTTAGGCAAAGGTGAGGGAATTGATGATTTAGCCCCCTTCACGGCCGCACAATATTCTTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.041

99.324

0.517