Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C2758_RS07200 Genome accession   NZ_CP061312
Coordinates   1370857..1372221 (-) Length   454 a.a.
NCBI ID   WP_215330177.1    Uniprot ID   -
Organism   Polynucleobacter sp. AP-Sving-400A-A2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1365857..1377221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2758_RS07185 (C2758_07175) dapD 1367911..1368738 (+) 828 WP_215307856.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  C2758_RS07190 (C2758_07180) dapE 1368756..1369907 (+) 1152 WP_215327577.1 succinyl-diaminopimelate desuccinylase -
  C2758_RS07195 (C2758_07185) prmB 1369948..1370844 (+) 897 WP_215327578.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  C2758_RS07200 (C2758_07190) radA 1370857..1372221 (-) 1365 WP_215330177.1 DNA repair protein RadA Machinery gene
  C2758_RS07205 (C2758_07195) - 1372226..1372735 (-) 510 WP_215327579.1 hypothetical protein -
  C2758_RS07210 (C2758_07200) - 1372732..1374672 (-) 1941 WP_215327580.1 potassium transporter Kup -
  C2758_RS07220 (C2758_07210) - 1375002..1375643 (-) 642 WP_215327581.1 DUF6803 family protein -
  C2758_RS07225 (C2758_07215) - 1375702..1376037 (-) 336 WP_215327582.1 hypothetical protein -
  C2758_RS07230 (C2758_07220) - 1376082..1376357 (-) 276 WP_251369167.1 addiction module antidote protein -
  C2758_RS07235 (C2758_07225) - 1376396..1376602 (-) 207 WP_371817691.1 type II toxin-antitoxin system HicB family antitoxin -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48118.88 Da        Isoelectric Point: 7.3471

>NTDB_id=481749 C2758_RS07200 WP_215330177.1 1370857..1372221(-) (radA) [Polynucleobacter sp. AP-Sving-400A-A2]
MAKIKTIYICQSCGGSSAKWQGQCPSCQAWNTLEEGVPEASSNSRFQGLAQSLPRQKLSAISAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSAAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLLSIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVANPSAIFLSQHAEMVPGACVLVTQEGSRPLLVEIQALVDTAHIPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNKALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPRTKIPGLRVIPVERIDQAISAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481749 C2758_RS07200 WP_215330177.1 1370857..1372221(-) (radA) [Polynucleobacter sp. AP-Sving-400A-A2]
TTGGCCAAAATAAAAACAATCTATATCTGTCAGTCGTGTGGTGGCAGCTCTGCTAAATGGCAGGGACAGTGCCCCTCATG
TCAGGCATGGAATACCTTGGAAGAGGGTGTTCCCGAGGCCAGCTCAAATTCCCGCTTTCAGGGGCTGGCACAGTCACTTC
CCCGTCAAAAACTATCCGCTATTTCGGCTGAAGATCTCCCACGCTTTAGTACTGGCGTAGAAGAGTTTGACCGCGTATTG
GGTGGCGGATTAGTTCCTGGTGGCGTTGTTCTTTTAGGCGGCGATCCTGGGATTGGTAAATCTACCCTCTTACTCCAAGC
CCTTGCTGAGATGAGTGCTGCTGGCATGAACGTGCTCTACAGCAGTGGCGAAGAGTCTGCCGCTCAGATTGCATTACGCG
CAAAACGTATTGCACTCGATGCACCTCAATTAGAAGTGCTGGCTGAGATTCAGTTGGAAAAGCTCTTATCTATTATGGAT
ACAGTGAAGCCACAGGTCTTGGTGGTCGATTCCATTCAGACTTTGTATTCAGAGGTGCTCAGCTCAGCTCCAGGCTCGGT
TGCCCAAGTGCGAGAGTGCGCTGCGCAATTAACCAGAGCCGCTAAATCTAGCGGCATTTGCGTGTTAATGGTGGGGCACG
TGACTAAAGATGGCCATTTAGCTGGCCCTCGTGTGCTCGAGCATATTGTAGATACCGTCTTATATTTCGAGGGTGATACC
CACTCCTCATTTAGATTAGTACGCTCGATTAAAAACCGCTTTGGCGCAGTCAACGAGTTAGGTGTATTTGCGATGACCGA
AAAAGGTCTGCGTGGCGTCGCCAATCCTTCAGCCATTTTCTTATCGCAGCATGCGGAGATGGTGCCAGGTGCCTGCGTAT
TGGTTACGCAAGAGGGTAGTCGACCGCTCTTAGTCGAAATTCAGGCGCTGGTAGATACGGCGCACATTCCTAATCCACGT
CGCCTGGCAGTTGGTTTAGAGCAAGCGCGTTTAGCGATGCTCTTAGCGGTACTACATCGTCATGCTGGTGTTGCCTGCTT
TGATCAAGACGTCTTCTTAAATGCAGTAGGTGGTGTAAAGATCTCAGAGCCGGCTGCTGACTTAGCGGTATTACTAGCAA
TTCAGTCTTCTATTCGCAATAAGGCCTTGCCTAAAGAGTTGATCGTATTTGGTGAGGTTGGTTTAGCTGGAGAGATTCGC
CCATGCCCACGTGGTCAGGAGCGCCTGAAAGAGGCGGCTAAGCTGGGCTTTACTGTAGCCATTATTCCGAAGGCCAATAT
GCCTAGGACCAAGATCCCAGGGTTAAGAGTGATACCAGTAGAACGTATTGATCAGGCCATCTCTGCAGCAGCAGAGCTCA
GTTAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(78-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.231

100

0.493
  radA Streptococcus pneumoniae Rx1

46.222

99.119

0.458
  radA Streptococcus pneumoniae D39

46.222

99.119

0.458
  radA Streptococcus pneumoniae R6

46.222

99.119

0.458
  radA Streptococcus pneumoniae TIGR4

46.222

99.119

0.458
  radA Streptococcus mitis NCTC 12261

46.222

99.119

0.458
  radA Streptococcus mitis SK321

46.222

99.119

0.458