Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FD974_RS08960 Genome accession   NZ_CP061310
Coordinates   1761134..1762024 (+) Length   296 a.a.
NCBI ID   WP_215364355.1    Uniprot ID   -
Organism   Polynucleobacter sp. es-EL-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1756134..1767024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD974_RS08930 (FD974_08925) pth 1756293..1756889 (+) 597 WP_215364347.1 aminoacyl-tRNA hydrolase -
  FD974_RS08935 (FD974_08930) - 1756919..1757185 (-) 267 WP_215364349.1 YfhL family 4Fe-4S dicluster ferredoxin -
  FD974_RS08940 (FD974_08935) coaD 1757204..1757701 (-) 498 WP_215364351.1 pantetheine-phosphate adenylyltransferase -
  FD974_RS08945 (FD974_08940) rsmD 1757738..1758358 (-) 621 WP_215366837.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  FD974_RS08950 (FD974_08945) - 1758351..1759697 (-) 1347 WP_215364353.1 pitrilysin family protein -
  FD974_RS08955 (FD974_08950) - 1759697..1761085 (-) 1389 WP_251374589.1 pitrilysin family protein -
  FD974_RS08960 (FD974_08955) pilA 1761134..1762024 (+) 891 WP_215364355.1 signal recognition particle-docking protein FtsY Machinery gene
  FD974_RS08965 (FD974_08960) rpoH 1762119..1763060 (+) 942 WP_215364357.1 RNA polymerase sigma factor RpoH -
  FD974_RS08970 (FD974_08965) - 1763069..1763680 (-) 612 WP_371817111.1 SCO family protein -
  FD974_RS08975 (FD974_08970) cyoE 1763682..1764575 (-) 894 WP_215364361.1 heme o synthase -
  FD974_RS08980 (FD974_08975) - 1764572..1765702 (-) 1131 WP_215364362.1 heme A synthase -
  FD974_RS08985 (FD974_08980) - 1765699..1766364 (-) 666 WP_251374591.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31730.30 Da        Isoelectric Point: 6.3515

>NTDB_id=481743 FD974_RS08960 WP_215364355.1 1761134..1762024(+) (pilA) [Polynucleobacter sp. es-EL-1]
MFGLRKTLGSLFKSNQTDEAWFDSLEESLILSDVGLPTTEQLMNKLRKAAKSERASSPEDLQQLLIQEVSKLLKPLEANP
NPLYEHDQKTLPQVWLVIGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVITQESGDAAA
VAHDAIHAAISRKTDILIIDTAGRLATQGNLMEELKKVKRVIGKALPGAPHQTLLVLDGNTGQNGLSQVKAFHAALDLTA
LIVTKLDGTAKGGVICALAQTLNEGDKPAVLALGKGEGIEDLAPFTAEQYSAEFFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481743 FD974_RS08960 WP_215364355.1 1761134..1762024(+) (pilA) [Polynucleobacter sp. es-EL-1]
ATGTTCGGATTACGTAAAACCCTCGGCTCCCTCTTTAAATCCAATCAGACTGACGAAGCCTGGTTTGATTCCCTAGAAGA
ATCTCTCATCCTGAGTGATGTAGGGCTTCCTACTACCGAGCAGCTCATGAACAAGCTGCGTAAAGCCGCCAAGTCAGAGC
GGGCAAGTAGCCCTGAAGATCTTCAGCAACTGTTGATTCAAGAGGTTTCCAAGCTACTCAAGCCTTTAGAGGCAAACCCA
AATCCCTTGTATGAGCATGATCAAAAGACCCTGCCACAGGTATGGCTAGTGATTGGGGTAAATGGTGCTGGCAAGACAAC
CACCATTGGCAAGCTCTGCAGACTTTTTCAATCTCAGGGCAAATCAGTCTTACTAGCGGCCGGAGACACCTTTCGTGCTG
CTGCCCGCAACCAATTGCTGGAATGGGGTGGCCGCAATCAGGTGGATGTGATCACTCAAGAAAGTGGAGATGCCGCTGCG
GTGGCCCACGATGCCATTCATGCGGCGATTTCTCGTAAAACCGATATTTTGATCATTGATACCGCGGGCAGACTAGCGAC
TCAAGGCAATCTCATGGAAGAGTTAAAAAAGGTCAAGCGAGTCATCGGCAAAGCCCTTCCTGGGGCTCCTCACCAGACTT
TGCTGGTTCTGGATGGCAATACCGGTCAAAACGGTTTAAGCCAAGTGAAGGCCTTTCATGCAGCCTTAGATTTAACCGCA
CTCATTGTGACCAAGCTAGATGGGACTGCTAAAGGAGGGGTCATTTGCGCCCTCGCCCAAACTCTCAATGAGGGGGATAA
ACCGGCCGTTTTAGCACTTGGAAAAGGCGAGGGAATTGAAGACCTAGCCCCTTTTACAGCTGAGCAATATTCAGCTGAAT
TTTTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-294)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.125

97.297

0.517