Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FD974_RS06390 Genome accession   NZ_CP061310
Coordinates   1251026..1252393 (-) Length   455 a.a.
NCBI ID   WP_215363532.1    Uniprot ID   -
Organism   Polynucleobacter sp. es-EL-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1246026..1257393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD974_RS06375 (FD974_06370) dapD 1248109..1248936 (+) 828 WP_215363526.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  FD974_RS06380 (FD974_06375) dapE 1248953..1250104 (+) 1152 WP_215363528.1 succinyl-diaminopimelate desuccinylase -
  FD974_RS06385 (FD974_06380) prmB 1250121..1251023 (+) 903 WP_215363530.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  FD974_RS06390 (FD974_06385) radA 1251026..1252393 (-) 1368 WP_215363532.1 DNA repair protein RadA Machinery gene
  FD974_RS06395 (FD974_06390) - 1252398..1252907 (-) 510 WP_215363534.1 hypothetical protein -
  FD974_RS06400 (FD974_06395) - 1252904..1254748 (-) 1845 WP_251374686.1 potassium transporter Kup -
  FD974_RS06410 (FD974_06405) - 1255317..1256408 (-) 1092 WP_215363539.1 hypothetical protein -
  FD974_RS06415 (FD974_06410) - 1256405..1257169 (-) 765 WP_215363541.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48236.07 Da        Isoelectric Point: 7.3470

>NTDB_id=481738 FD974_RS06390 WP_215363532.1 1251026..1252393(-) (radA) [Polynucleobacter sp. es-EL-1]
MAKIKTIYICQSCGGTSAKWQGQCPSCQAWNTLEEGLPEVSSNTRFQGLAQSLPRQKLSAISAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSASGMNVLYSSGEESAAQIALRAKRIALDAPRLEVLAEIQLEKLLSIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVANPSAIFLSQHAEMVPGACVLVTQEGSRPLLVEIQALVDTAHIPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNKALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIAGLTVIPVERIDQAIAAAAELSQ

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=481738 FD974_RS06390 WP_215363532.1 1251026..1252393(-) (radA) [Polynucleobacter sp. es-EL-1]
TTGGCCAAAATTAAAACAATCTATATTTGTCAATCTTGCGGCGGTACCTCTGCAAAATGGCAGGGCCAGTGCCCTTCATG
CCAAGCTTGGAATACGCTCGAAGAAGGCTTGCCTGAGGTCAGCTCCAATACGCGCTTTCAGGGCTTGGCGCAATCCCTGC
CAAGGCAAAAACTATCAGCTATTTCTGCCGAGGATCTGCCGAGATTTAGCACTGGAGTTGAAGAGTTTGATCGCGTCTTA
GGGGGTGGCCTTGTACCTGGTGGCGTAGTGCTTTTAGGTGGTGACCCGGGGATTGGTAAATCTACACTGCTACTGCAAGC
ACTTGCTGAAATGAGTGCTTCGGGCATGAATGTCCTCTATAGCAGTGGCGAAGAGTCTGCTGCGCAGATTGCCCTGCGCG
CTAAACGCATTGCTTTAGATGCTCCTCGGCTTGAAGTGTTAGCAGAGATTCAATTAGAAAAGCTTTTGTCCATTATGGAC
ACCGTAAAGCCGCAGGTATTAGTGGTTGATTCGATTCAAACTTTGTACTCAGAAGTCCTCAGTTCAGCGCCTGGTTCTGT
TGCGCAGGTGCGAGAATGTGCTGCGCAGTTAACGCGAGCAGCCAAGTCCAGCGGCATTTGCGTGTTGATGGTGGGCCATG
TAACCAAAGACGGTCACTTAGCTGGTCCCCGCGTTCTAGAACATATTGTCGATACTGTCCTGTATTTTGAGGGAGATACC
CACTCTTCCTTTAGATTGGTACGTTCAATTAAAAACCGTTTTGGTGCTGTCAATGAGTTGGGTGTTTTTGCCATGACAGA
AAAAGGTTTGCGAGGCGTTGCCAATCCTTCTGCGATTTTTCTGTCGCAACATGCAGAAATGGTTCCCGGTGCTTGCGTAT
TGGTAACACAAGAGGGCAGTCGCCCTTTGCTGGTAGAAATTCAAGCATTGGTAGATACGGCGCATATTCCGAATCCAAGA
CGATTGGCGGTAGGCCTTGAGCAAGCCCGCCTAGCAATGCTCTTAGCAGTGTTGCATCGTCATGCAGGCGTAGCTTGTTT
TGATCAAGATGTCTTTTTAAATGCGGTTGGCGGGGTCAAAATCTCTGAGCCTGCAGCAGACTTGGCAGTCTTATTGGCTA
TCCAGTCTTCGATTCGGAATAAAGCATTGCCAAAAGAGTTGATTGTTTTTGGTGAAGTAGGCTTGGCAGGTGAGATTCGT
CCCTGCCCACGAGGTCAGGAGCGCTTGAAAGAAGCGGCTAAGCTAGGCTTTACTGTAGCCATTATTCCGAAGGCCAATAT
GCCCAAGACCAAGATTGCTGGCTTAACGGTGATACCCGTAGAACGCATTGATCAAGCTATTGCGGCAGCAGCAGAACTAA
GTCAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.558

99.341

0.492
  radA Streptococcus mitis NCTC 12261

46.188

98.022

0.453
  radA Streptococcus pneumoniae Rx1

46.188

98.022

0.453
  radA Streptococcus pneumoniae D39

46.188

98.022

0.453
  radA Streptococcus pneumoniae R6

46.188

98.022

0.453
  radA Streptococcus pneumoniae TIGR4

46.188

98.022

0.453
  radA Streptococcus mitis SK321

46.575

96.264

0.448