Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ICV90_RS09495 Genome accession   NZ_CP061306
Coordinates   1879535..1880425 (+) Length   296 a.a.
NCBI ID   WP_215358684.1    Uniprot ID   -
Organism   Polynucleobacter sp. JS-JIR-II-b4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1874535..1885425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICV90_RS09465 (ICV90_09485) pth 1874729..1875319 (+) 591 WP_215358680.1 aminoacyl-tRNA hydrolase -
  ICV90_RS09470 (ICV90_09490) - 1875325..1875591 (-) 267 WP_215358681.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ICV90_RS09475 (ICV90_09495) coaD 1875617..1876114 (-) 498 WP_215358682.1 pantetheine-phosphate adenylyltransferase -
  ICV90_RS09480 (ICV90_09500) rsmD 1876151..1876723 (-) 573 WP_251367724.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ICV90_RS09485 (ICV90_09505) - 1876770..1878110 (-) 1341 WP_251367725.1 pitrilysin family protein -
  ICV90_RS09490 (ICV90_09510) - 1878107..1879471 (-) 1365 WP_215358683.1 pitrilysin family protein -
  ICV90_RS09495 (ICV90_09515) pilA 1879535..1880425 (+) 891 WP_215358684.1 signal recognition particle-docking protein FtsY Machinery gene
  ICV90_RS09500 (ICV90_09520) rpoH 1880519..1881442 (+) 924 WP_215358685.1 RNA polymerase sigma factor RpoH -
  ICV90_RS09505 (ICV90_09525) - 1881484..1882068 (-) 585 WP_215358686.1 SCO family protein -
  ICV90_RS09510 (ICV90_09530) cyoE 1882076..1882969 (-) 894 WP_215358687.1 heme o synthase -
  ICV90_RS09515 (ICV90_09535) - 1882972..1884093 (-) 1122 WP_215358688.1 heme A synthase -
  ICV90_RS09520 (ICV90_09540) - 1884093..1884749 (-) 657 WP_215358689.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31444.04 Da        Isoelectric Point: 6.4998

>NTDB_id=481732 ICV90_RS09495 WP_215358684.1 1879535..1880425(+) (pilA) [Polynucleobacter sp. JS-JIR-II-b4]
MFGLRKTLGSLFKSSKIDESWFETLEESLIQSDVGLPTTEQLITKLRKAAKSEKASSPEELQALLIQEVSALLTAIEPLP
NPLFTSDKPNTPEVWLVVGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHAAISRKNDILIIDTAGRLATQDNLMEELKKVKRVIGKVLPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQDGPKPAVLALGKGEGIEDLAPFTAEQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481732 ICV90_RS09495 WP_215358684.1 1879535..1880425(+) (pilA) [Polynucleobacter sp. JS-JIR-II-b4]
ATGTTCGGCTTACGTAAAACCCTCGGATCCCTTTTCAAATCAAGCAAGATTGATGAATCTTGGTTTGAAACTTTAGAAGA
ATCACTCATTCAAAGTGATGTGGGTCTACCTACGACTGAACAACTCATCACCAAACTACGCAAAGCAGCCAAGTCTGAAA
AGGCTTCTAGTCCAGAAGAGTTGCAAGCTCTTCTCATTCAAGAAGTCAGCGCCCTATTAACTGCTATTGAACCTTTACCA
AACCCACTGTTTACAAGTGATAAGCCTAACACCCCAGAGGTATGGTTGGTTGTAGGGGTAAATGGAGCCGGCAAGACTAC
GACCATTGGCAAACTCTGCCGACTCTTTCAGTCTCAAGGTAAGTCAGTTCTTTTAGCTGCAGGCGATACCTTTAGAGCTG
CGGCACGTAATCAGCTTCAGGAGTGGGGTGGACGCAACCAGGTTGACGTTATTACTCAAGAAGGTGGTGATGCAGCAGCC
GTTGCACACGACGCCATTCATGCTGCCATCTCTCGCAAGAACGATATTCTCATCATTGACACTGCAGGCCGTCTTGCTAC
CCAAGACAACCTCATGGAAGAGCTTAAGAAAGTAAAACGCGTCATTGGTAAAGTGCTTCCTGGGGCACCTCATCACACCC
TGCTTGTTTTGGATGGCAATACCGGACAGAATGGCTTGAGCCAGGTAAAGGCCTTCCATGCCGCTCTTGGCCTAACTGGA
ATCATCGTTACAAAGCTTGATGGCACTGCTAAGGGCGGCGTGATCTGCGCCCTAGCCCACACCCTACAAGATGGCCCAAA
ACCCGCTGTTCTAGCCCTAGGCAAGGGCGAAGGGATTGAAGATTTAGCCCCCTTCACAGCAGAACAATATTCATCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.242

98.986

0.527