Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C2740_RS08550 Genome accession   NZ_CP061304
Coordinates   1675131..1676021 (+) Length   296 a.a.
NCBI ID   WP_215293240.1    Uniprot ID   -
Organism   Polynucleobacter sp. MG-5-Ahmo-C2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1670131..1681021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2740_RS08520 (C2740_08525) pth 1670350..1670940 (+) 591 WP_215293231.1 aminoacyl-tRNA hydrolase -
  C2740_RS08525 (C2740_08530) - 1670937..1671203 (-) 267 WP_215293233.1 YfhL family 4Fe-4S dicluster ferredoxin -
  C2740_RS08530 (C2740_08535) coaD 1671219..1671716 (-) 498 WP_215293235.1 pantetheine-phosphate adenylyltransferase -
  C2740_RS08535 (C2740_08540) rsmD 1671753..1672391 (-) 639 WP_251369634.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  C2740_RS08540 (C2740_08545) - 1672363..1673700 (-) 1338 WP_215293236.1 pitrilysin family protein -
  C2740_RS08545 (C2740_08550) - 1673700..1675067 (-) 1368 WP_215293238.1 pitrilysin family protein -
  C2740_RS08550 (C2740_08555) pilA 1675131..1676021 (+) 891 WP_215293240.1 signal recognition particle-docking protein FtsY Machinery gene
  C2740_RS08555 (C2740_08560) rpoH 1676112..1677041 (+) 930 WP_215293241.1 RNA polymerase sigma factor RpoH -
  C2740_RS08560 (C2740_08565) - 1677067..1677657 (-) 591 WP_215293243.1 SCO family protein -
  C2740_RS08565 (C2740_08570) cyoE 1677659..1678552 (-) 894 WP_215293245.1 heme o synthase -
  C2740_RS08570 (C2740_08575) - 1678549..1679676 (-) 1128 WP_215293247.1 heme A synthase -
  C2740_RS08575 (C2740_08580) - 1679676..1680332 (-) 657 WP_215293249.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31474.15 Da        Isoelectric Point: 7.4793

>NTDB_id=481721 C2740_RS08550 WP_215293240.1 1675131..1676021(+) (pilA) [Polynucleobacter sp. MG-5-Ahmo-C2]
MFGLRKTLGSLFKSSKTDEAWFDTLEESLIQSDVGLPTTEQLIGKLRKAAKSEKASSPEELQTLLIEEVSTLLKALEPSP
NPLFESIKANIPEIWLVVGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLQEWGSRNQVDVITQEGGDAAA
VAHDAIHSAISRKNDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLILDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQEGGKPAILALGKGEGIDDLAPFTAKRYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481721 C2740_RS08550 WP_215293240.1 1675131..1676021(+) (pilA) [Polynucleobacter sp. MG-5-Ahmo-C2]
ATGTTCGGCTTACGTAAAACCCTCGGATCCCTTTTCAAGTCAAGCAAAACAGATGAAGCTTGGTTTGATACTTTGGAAGA
ATCACTAATTCAAAGTGATGTGGGCCTACCTACGACTGAGCAATTGATAGGTAAGCTTCGCAAAGCTGCTAAATCAGAAA
AAGCTTCTAGTCCTGAAGAGCTACAAACGCTTCTCATTGAAGAAGTAAGCACCCTACTAAAAGCATTAGAGCCCTCCCCA
AACCCTCTTTTTGAATCTATCAAAGCCAATATCCCGGAGATATGGCTAGTTGTGGGAGTCAATGGAGCTGGCAAGACCAC
CACTATTGGCAAACTTTGTAAGCTCTTTCAATCTCAAGGAAAATCTGTTCTCTTGGCTGCAGGAGATACATTTAGAGCTG
CTGCCCGGAATCAATTACAAGAATGGGGTAGTCGCAATCAGGTAGACGTCATTACTCAGGAAGGCGGTGATGCCGCTGCA
GTTGCTCATGACGCCATCCACTCAGCAATCTCTCGTAAAAACGATATCCTGATTATTGATACTGCTGGTCGCCTTGCCAC
TCAAGACCACCTCATGGAAGAACTCAAGAAGGTAAAGCGTGTGATCGGCAAAGCCCTTCCTGGCGCACCACACCATACCC
TGCTAATACTGGATGGCAATACTGGTCAAAACGGCTTGAGTCAAGTAAAGGCCTTCCATGCGGCCCTTGGTCTTACTGGA
ATCATCGTTACGAAGCTAGATGGCACTGCTAAGGGTGGCGTTATCTGCGCCCTGGCACATACCCTCCAAGAAGGAGGAAA
GCCAGCCATATTGGCCCTAGGCAAGGGCGAGGGAATTGATGATTTAGCGCCATTCACAGCTAAGCGATATTCATCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(93-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.701

99.324

0.514