Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C2740_RS02555 Genome accession   NZ_CP061304
Coordinates   475267..476631 (+) Length   454 a.a.
NCBI ID   WP_215293849.1    Uniprot ID   -
Organism   Polynucleobacter sp. MG-5-Ahmo-C2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 470267..481631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2740_RS02530 (C2740_02530) - 471456..471833 (+) 378 WP_251369675.1 glycine zipper family protein -
  C2740_RS02535 (C2740_02535) - 471950..472489 (+) 540 WP_215293846.1 hypothetical protein -
  C2740_RS02545 (C2740_02545) - 472779..474722 (+) 1944 WP_215293847.1 potassium transporter Kup -
  C2740_RS02550 (C2740_02550) - 474719..475225 (+) 507 WP_215293848.1 hypothetical protein -
  C2740_RS02555 (C2740_02555) radA 475267..476631 (+) 1365 WP_215293849.1 DNA repair protein RadA Machinery gene
  C2740_RS02560 (C2740_02560) prmB 476639..477535 (-) 897 WP_215293850.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  C2740_RS02565 (C2740_02565) dapE 477572..478723 (-) 1152 WP_215293851.1 succinyl-diaminopimelate desuccinylase -
  C2740_RS02570 (C2740_02570) dapD 478730..479557 (-) 828 WP_215293852.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48265.08 Da        Isoelectric Point: 7.3470

>NTDB_id=481713 C2740_RS02555 WP_215293849.1 475267..476631(+) (radA) [Polynucleobacter sp. MG-5-Ahmo-C2]
MAKVKTVYICQSCGGTSAKWQGQCPSCQAWNTMEEGLPETTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGIACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKAKIPGLKLIPVERIDQAIAAAAELN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481713 C2740_RS02555 WP_215293849.1 475267..476631(+) (radA) [Polynucleobacter sp. MG-5-Ahmo-C2]
TTGGCTAAAGTCAAAACGGTTTATATCTGCCAGTCTTGTGGCGGCACTTCTGCAAAGTGGCAAGGACAATGCCCATCTTG
CCAGGCGTGGAACACGATGGAAGAGGGCTTGCCTGAAACAACATCTAATTCTCGTTTTCAGGGGTTGGCGCAATCTTTGC
CTAGGCAAAAACTTTCTGCTATTACTGCTGAGGACTTGCCAAGATTCAGTACCGGCGTTGAAGAGTTTGATCGCGTCTTG
GGCGGCGGCTTAGTTCCTGGCGGCGTGGTTCTTTTGGGCGGCGATCCTGGTATTGGAAAATCAACCTTGTTATTGCAAGC
CTTAGCAGAGATGAGTTCTGCCGGTATGAATGTGCTCTACAGTAGTGGTGAAGAATCGGCTGCCCAGATTGCCTTGCGTG
CAAAACGAATTGCATTAGATGCTCCGCAATTGGAAGTCTTAGCAGAAATTCAGTTAGAAAAACTCATTTCGATTATGGAT
ACCGTTAAGCCACAAGTATTGGTGGTGGATTCTATACAAACTTTGTACTCCGAAGTGCTCAGCTCTGCCCCTGGATCTGT
AGCGCAAGTGCGTGAGTGTGCTGCGCAATTGACAAGAGCCGCTAAATCCAGTGGTATCTGCGTGCTGATGGTGGGTCACG
TTACCAAAGATGGTCATCTAGCTGGTCCAAGGGTCCTAGAACATATTGTGGATACTGTTTTGTACTTTGAGGGTGATACC
CATTCTTCGTTTCGACTGGTGCGCTCGATTAAGAATCGTTTTGGTGCCGTTAATGAGCTGGGTGTATTTGCAATGACCGA
AAAAGGTTTGCGCGGCGTCACCAACCCTTCTGCAATTTTTCTCTCTCAGCACGAGCAGATGGTTCCAGGAGCATGCGTTT
TAGTGACGCAAGAAGGTAGCAGACCATTGCTTGTAGAAATTCAGGCGCTAGTAGATACCGCTCACGTTCCTAATCCAAGG
CGCTTGGCAGTTGGTTTAGAACAAGCTCGCTTAGCAATGCTCTTAGCTGTCTTGCATCGTCATGCGGGTATAGCTTGCTT
TGATCAAGATGTATTCCTGAATGCGGTGGGCGGCGTCAAAATCTCTGAGCCTGCAGCTGACTTAGCGGTATTGCTAGCTA
TTCAGTCATCGATCCGAAATCGCGCTCTACCTAAAGAGTTGATTGTGTTTGGTGAGGTTGGCTTAGCAGGGGAGATTCGT
CCATGCCCGCGTGGTCAAGAACGTTTAAAAGAAGCGGCCAAGCTGGGTTTTACAGTAGCCATTATTCCAAAAGCGAATAT
GCCTAAGGCTAAGATTCCAGGATTAAAGCTTATTCCGGTGGAGCGTATTGATCAGGCTATTGCTGCCGCAGCAGAATTGA
ACTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.115

99.559

0.489
  radA Streptococcus pneumoniae Rx1

45.556

99.119

0.452
  radA Streptococcus pneumoniae D39

45.556

99.119

0.452
  radA Streptococcus pneumoniae R6

45.556

99.119

0.452
  radA Streptococcus pneumoniae TIGR4

45.556

99.119

0.452
  radA Streptococcus mitis NCTC 12261

46.853

94.493

0.443
  radA Streptococcus mitis SK321

46.853

94.493

0.443