Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GQ367_RS04195 Genome accession   NZ_CP061299
Coordinates   822004..823368 (+) Length   454 a.a.
NCBI ID   WP_089516619.1    Uniprot ID   A0A229FRC2
Organism   Polynucleobacter sp. MWH-CaK5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 817004..828368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ367_RS04170 (GQ367_04165) - 817643..818194 (-) 552 WP_215291756.1 peptidylprolyl isomerase -
  GQ367_RS04180 (GQ367_04175) - 818503..819453 (+) 951 WP_215291758.1 MBL fold metallo-hydrolase -
  GQ367_RS04185 (GQ367_04180) - 819566..821467 (+) 1902 WP_251370228.1 potassium transporter Kup -
  GQ367_RS04190 (GQ367_04185) - 821510..822004 (+) 495 WP_215291762.1 hypothetical protein -
  GQ367_RS04195 (GQ367_04190) radA 822004..823368 (+) 1365 WP_089516619.1 DNA repair protein RadA Machinery gene
  GQ367_RS04200 (GQ367_04195) prmB 823389..824270 (-) 882 WP_215291764.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  GQ367_RS04205 (GQ367_04200) dapE 824294..825451 (-) 1158 WP_371818552.1 succinyl-diaminopimelate desuccinylase -
  GQ367_RS04210 (GQ367_04205) dapD 825474..826307 (-) 834 WP_215291766.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48209.89 Da        Isoelectric Point: 7.6022

>NTDB_id=481690 GQ367_RS04195 WP_089516619.1 822004..823368(+) (radA) [Polynucleobacter sp. MWH-CaK5]
MAKIKSVYVCQACGGLSAKWQGQCPNCEAWNSMEETVEDKPSTNRFQSLAKAAPKQKLSVIEAEDLPRISTGVDEFDRVL
GGGLVTGGVALIGGDPGIGKSTLLLQALAAMSARGDSVLYISGEESGAQIALRAKRLGIQAPDLEVLAEIQLEKLLATIE
SARPTVVVVDSIQTVYSEALSSAPGSVAQVKECAAQLTRLAKTTGVCMIMVGHVTKEGNLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRAFKNRFGAVNELGVFAMTDKGLKGVANPSALFLSQHDQIVPGSCVLVTQEGSRPLLVEIQALVDAAHVPNPR
RLALGLEQHRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIQSSMKNKALPKNLIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFKQAIVPKANAPKTNIPGLTIFAVDRIDQAIQAARNLE

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481690 GQ367_RS04195 WP_089516619.1 822004..823368(+) (radA) [Polynucleobacter sp. MWH-CaK5]
ATGGCGAAAATTAAATCTGTTTATGTTTGCCAGGCTTGTGGAGGTCTATCAGCCAAGTGGCAGGGGCAGTGTCCCAACTG
CGAGGCTTGGAATTCCATGGAAGAAACTGTGGAAGACAAGCCTTCAACTAATCGCTTTCAAAGCTTGGCAAAAGCTGCAC
CAAAACAAAAACTATCCGTTATAGAAGCTGAGGATTTACCGCGCATCAGCACAGGTGTTGATGAGTTTGATCGGGTGTTG
GGTGGTGGTTTGGTCACTGGTGGTGTTGCATTGATTGGTGGAGACCCTGGTATTGGTAAATCCACTTTGCTTTTGCAAGC
GCTCGCAGCAATGAGTGCTAGAGGTGATTCTGTTCTTTACATCAGCGGGGAAGAGTCCGGCGCGCAAATTGCTTTGAGAG
CAAAGCGCTTGGGAATACAGGCTCCTGATCTAGAAGTATTGGCAGAAATCCAGCTAGAAAAATTATTGGCGACCATTGAA
TCAGCCAGACCTACTGTGGTTGTTGTTGATTCGATACAAACCGTTTATTCAGAAGCTTTGAGTTCAGCTCCTGGTTCAGT
GGCTCAGGTCAAAGAATGCGCAGCCCAACTGACACGCTTGGCCAAAACAACAGGTGTTTGCATGATCATGGTGGGCCATG
TCACCAAAGAAGGTAATTTGGCAGGCCCTAGAGTCTTAGAACACATCGTTGACACAGTTTTGTATTTTGAAGGCGATACG
CACTCATCGTTTAGGTTGGTTCGAGCCTTTAAAAACCGTTTTGGTGCTGTGAATGAATTGGGTGTTTTTGCCATGACCGA
TAAAGGTCTTAAAGGTGTGGCGAATCCATCAGCTTTATTCTTGTCGCAGCACGATCAAATCGTTCCTGGTTCTTGTGTGT
TGGTGACGCAAGAGGGCAGTCGACCATTGTTGGTTGAGATTCAAGCATTGGTGGATGCAGCCCATGTTCCCAATCCACGC
AGATTGGCTTTAGGTTTAGAGCAACACCGTTTGGCCATGCTTTTAGCCGTACTTCATCGTCATGCAGGCATTGCTTGTTT
TGATCAAGATGTCTTCTTGAATGCGGTAGGTGGTGTAAAAATCACCGAGCCAGCAGCTGACTTGGCAGTTTTATTAGCTA
TCCAGTCATCCATGAAAAATAAAGCATTGCCAAAGAATTTAATTGTCTTTGGTGAGGTTGGTTTGGCTGGAGAAATCAGA
CCATGTCCAAGAGGTCAGGAGCGTCTCAAAGAAGCTGCCAAGTTAGGCTTTAAGCAGGCCATCGTTCCTAAGGCCAATGC
CCCTAAAACAAACATACCTGGCTTAACTATTTTTGCTGTTGATCGAATTGATCAGGCCATTCAAGCCGCCAGAAATCTAG
AGTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-218)

Subunit ChlI of Mg-chelatase (ChlI)

(359-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A229FRC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.563

100

0.5
  radA Streptococcus pneumoniae Rx1

48.387

95.595

0.463
  radA Streptococcus pneumoniae D39

48.387

95.595

0.463
  radA Streptococcus pneumoniae R6

48.387

95.595

0.463
  radA Streptococcus pneumoniae TIGR4

48.387

95.595

0.463
  radA Streptococcus mitis SK321

49.176

93.612

0.46
  radA Streptococcus mitis NCTC 12261

48.941

93.612

0.458