Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AOC19_RS06140 Genome accession   NZ_CP061297
Coordinates   1238363..1239727 (-) Length   454 a.a.
NCBI ID   WP_215374871.1    Uniprot ID   -
Organism   Polynucleobacter asymbioticus strain MWH-MoIso1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1233363..1244727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOC19_RS06125 (AOC19_06130) dapD 1235429..1236256 (+) 828 WP_015421440.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  AOC19_RS06130 (AOC19_06135) dapE 1236266..1237417 (+) 1152 WP_215374865.1 succinyl-diaminopimelate desuccinylase -
  AOC19_RS06135 (AOC19_06140) prmB 1237454..1238350 (+) 897 WP_215374868.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  AOC19_RS06140 (AOC19_06145) radA 1238363..1239727 (-) 1365 WP_215374871.1 DNA repair protein RadA Machinery gene
  AOC19_RS06145 (AOC19_06150) - 1239773..1240282 (-) 510 WP_215374874.1 hypothetical protein -
  AOC19_RS06150 (AOC19_06155) - 1240279..1242120 (-) 1842 WP_251368095.1 potassium transporter Kup -
  AOC19_RS06160 (AOC19_06165) - 1242567..1243139 (-) 573 WP_215374880.1 hypothetical protein -
  AOC19_RS06165 (AOC19_06170) - 1243640..1243975 (+) 336 WP_215374883.1 hypothetical protein -
  AOC19_RS06170 (AOC19_06175) - 1243959..1244627 (-) 669 WP_215374886.1 recombinase family protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48157.01 Da        Isoelectric Point: 7.3470

>NTDB_id=481669 AOC19_RS06140 WP_215374871.1 1238363..1239727(-) (radA) [Polynucleobacter asymbioticus strain MWH-MoIso1]
MAKIKTIYICQSCGGTSAKWQGQCPSCQAWNTLEEGLPEVSSNPRFQGLAQSLPRQKLSAISAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSAAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLLSIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVANPSAIFLSQHAEMVPGACVLVTQEGSRPLLVEIQALVDTAHIPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNKALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLRVIPVERIDQAISAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481669 AOC19_RS06140 WP_215374871.1 1238363..1239727(-) (radA) [Polynucleobacter asymbioticus strain MWH-MoIso1]
TTGGCCAAGATTAAAACAATCTATATCTGTCAATCCTGTGGAGGCACCTCTGCTAAATGGCAAGGGCAGTGCCCTTCATG
TCAGGCGTGGAATACGCTGGAGGAGGGCTTGCCGGAGGTGAGTTCGAATCCCCGCTTTCAAGGTCTGGCGCAGTCACTTC
CTCGTCAAAAGCTCTCCGCTATCAGCGCAGAAGATCTTCCACGCTTTAGTACTGGCGTAGAGGAGTTTGATCGCGTACTG
GGTGGTGGATTGGTTCCTGGCGGAGTCGTTTTATTGGGTGGTGATCCCGGCATTGGTAAATCGACCTTATTGCTTCAAGC
ACTCGCTGAGATGAGTGCTGCTGGCATGAATGTTCTTTACAGTAGCGGTGAAGAATCTGCTGCACAAATCGCTTTACGTG
CAAAACGTATCGCATTGGATGCGCCCCAATTAGAAGTACTCGCAGAGATACAGCTAGAAAAGCTTCTATCCATCATGGAT
ACGGTGAAGCCGCAGGTCTTAGTGGTGGACTCCATTCAGACCTTGTATTCCGAGGTACTGAGTTCTGCTCCAGGTTCTGT
GGCGCAGGTAAGGGAGTGTGCTGCGCAATTAACGAGAGCTGCTAAATCCAGCGGTATCTGTGTATTGATGGTGGGTCACG
TGACTAAAGATGGCCACTTAGCTGGTCCTCGCGTGCTTGAGCATATTGTGGATACCGTTCTCTATTTTGAGGGTGATACT
CACTCTTCATTTAGATTGGTACGCTCCATTAAAAATCGCTTTGGCGCAGTCAATGAGCTGGGCGTATTTGCCATGACCGA
AAAAGGTCTACGTGGCGTTGCTAATCCCTCAGCGATTTTCCTATCTCAGCATGCGGAGATGGTGCCTGGTGCTTGTGTGT
TGGTTACACAAGAAGGTAGTCGTCCACTCTTGGTAGAGATTCAGGCGCTCGTAGATACTGCGCATATTCCGAATCCACGC
CGTTTGGCCGTTGGTTTGGAGCAGGCGCGCTTGGCAATGCTCTTAGCGGTATTGCATCGTCACGCAGGTGTCGCCTGCTT
TGATCAAGATGTCTTCTTGAATGCCGTTGGTGGCGTGAAGATCTCAGAGCCGGCAGCTGACTTAGCAGTGCTGTTAGCAA
TCCAATCTTCTATTCGTAATAAAGCGCTTCCAAAAGAGTTAATCGTATTCGGAGAGGTTGGTTTGGCTGGAGAGATTCGT
CCCTGCCCACGTGGTCAGGAGCGCCTGAAAGAAGCAGCTAAGCTTGGCTTTACTGTGGCCATCATTCCGAAGGCCAATAT
GCCGAAGACAAAGATTCCAGGATTAAGAGTGATACCAGTAGAGCGTATTGATCAAGCCATCTCTGCAGCAGCTGAGCTGA
GCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(78-218)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.011

100

0.491
  radA Streptococcus pneumoniae Rx1

45.778

99.119

0.454
  radA Streptococcus pneumoniae D39

45.778

99.119

0.454
  radA Streptococcus pneumoniae R6

45.778

99.119

0.454
  radA Streptococcus pneumoniae TIGR4

45.778

99.119

0.454
  radA Streptococcus mitis NCTC 12261

45.778

99.119

0.454
  radA Streptococcus mitis SK321

45.778

99.119

0.454