Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ICU98_RS08080 Genome accession   NZ_CP061296
Coordinates   1561155..1562045 (+) Length   296 a.a.
NCBI ID   WP_215352026.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-P3-07-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1556155..1567045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICU98_RS08050 (ICU98_08040) pth 1556374..1556964 (+) 591 WP_215352017.1 aminoacyl-tRNA hydrolase -
  ICU98_RS08055 (ICU98_08045) - 1556961..1557224 (-) 264 WP_215352019.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ICU98_RS08060 (ICU98_08050) coaD 1557243..1557734 (-) 492 WP_215336393.1 pantetheine-phosphate adenylyltransferase -
  ICU98_RS08065 (ICU98_08055) rsmD 1557770..1558387 (-) 618 WP_215352021.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ICU98_RS08070 (ICU98_08060) - 1558390..1559727 (-) 1338 WP_215352023.1 pitrilysin family protein -
  ICU98_RS08075 (ICU98_08065) - 1559724..1561094 (-) 1371 WP_215352025.1 pitrilysin family protein -
  ICU98_RS08080 (ICU98_08070) pilA 1561155..1562045 (+) 891 WP_215352026.1 signal recognition particle-docking protein FtsY Machinery gene
  ICU98_RS08085 (ICU98_08075) rpoH 1562138..1563079 (+) 942 WP_215352027.1 RNA polymerase sigma factor RpoH -
  ICU98_RS08090 (ICU98_08080) - 1563090..1563680 (-) 591 WP_215352028.1 SCO family protein -
  ICU98_RS08095 (ICU98_08085) cyoE 1563682..1564575 (-) 894 WP_215352029.1 heme o synthase -
  ICU98_RS08100 (ICU98_08090) - 1564572..1565702 (-) 1131 WP_215352030.1 heme A synthase -
  ICU98_RS08105 (ICU98_08095) - 1565702..1566358 (-) 657 WP_215352031.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31923.62 Da        Isoelectric Point: 6.7752

>NTDB_id=481663 ICU98_RS08080 WP_215352026.1 1561155..1562045(+) (pilA) [Polynucleobacter sp. MWH-P3-07-1]
MLGLRKTLGSLFKSSRIDEAWFDTLEESLIQSDVGMPTCMQLMASLRQAAKSEKVQDSETLQALLIQELTKLLQPLEAHP
NPIFEHQNHHHPEIWLIVGVNGAGKTTTIGKLCKQFQSQGKSILLAAGDTFRAAARNQLLEWGQRNQVDVIAQESGDAAA
VAHDAIHAAISRKTDILIIDTAGRLATQDHLMEELKKVKRVIAKALPGAPHHTLLILDGNTGQNGLNQVRAFHDALGLTG
LIVSKLDGTAKGGVICALAHTFESGSKPVVLGLGKGEGIEDLIPFTASQYSAELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481663 ICU98_RS08080 WP_215352026.1 1561155..1562045(+) (pilA) [Polynucleobacter sp. MWH-P3-07-1]
ATGCTCGGCCTACGTAAAACCCTCGGATCGCTTTTTAAATCAAGCCGGATTGATGAAGCTTGGTTTGATACTCTGGAAGA
GTCCTTGATTCAGAGCGATGTGGGCATGCCAACCTGTATGCAATTGATGGCCAGCTTACGTCAAGCCGCCAAATCAGAAA
AAGTCCAAGACTCAGAAACCCTACAAGCATTATTGATTCAAGAGCTTACTAAGCTATTACAGCCATTGGAGGCTCACCCT
AACCCAATCTTTGAGCATCAAAACCATCATCATCCTGAAATCTGGCTGATTGTTGGCGTCAATGGCGCAGGTAAAACCAC
CACCATTGGTAAGCTCTGTAAACAATTTCAGTCACAGGGCAAATCGATTCTCTTAGCTGCTGGCGACACCTTCCGTGCAG
CTGCCCGCAATCAACTCTTGGAGTGGGGACAGCGTAATCAGGTCGATGTCATTGCCCAAGAAAGTGGCGATGCTGCGGCT
GTGGCGCACGATGCTATTCATGCTGCAATCTCGCGCAAGACCGATATCTTGATCATTGACACTGCGGGTAGACTAGCCAC
ACAAGACCATTTGATGGAAGAGTTGAAGAAAGTAAAACGCGTCATTGCTAAGGCCCTCCCAGGAGCGCCTCATCACACAC
TCTTGATCTTGGATGGCAATACTGGTCAGAATGGTTTAAATCAAGTCAGGGCCTTTCATGACGCTCTAGGGCTCACCGGC
CTGATTGTTAGCAAACTCGATGGAACTGCCAAAGGCGGGGTCATTTGCGCCCTTGCTCACACCTTTGAAAGCGGCTCCAA
ACCAGTAGTTCTCGGGCTGGGCAAAGGCGAGGGAATTGAGGATCTAATCCCCTTTACTGCCTCTCAATATTCAGCAGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(93-295)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-69)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.701

99.324

0.514