Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ICU98_RS03145 Genome accession   NZ_CP061296
Coordinates   593582..594946 (+) Length   454 a.a.
NCBI ID   WP_215352718.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-P3-07-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 588582..599946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICU98_RS03120 (ICU98_03115) - 588971..589354 (+) 384 WP_215352714.1 hypothetical protein -
  ICU98_RS03125 (ICU98_03120) - 589397..590077 (+) 681 WP_215352715.1 hypothetical protein -
  ICU98_RS03135 (ICU98_03130) - 591104..593044 (+) 1941 WP_215335406.1 potassium transporter Kup -
  ICU98_RS03140 (ICU98_03135) - 593041..593544 (+) 504 WP_215352717.1 hypothetical protein -
  ICU98_RS03145 (ICU98_03140) radA 593582..594946 (+) 1365 WP_215352718.1 DNA repair protein RadA Machinery gene
  ICU98_RS03150 (ICU98_03145) prmB 594943..595842 (-) 900 WP_215352720.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  ICU98_RS03155 (ICU98_03150) dapE 595872..597023 (-) 1152 WP_215352721.1 succinyl-diaminopimelate desuccinylase -
  ICU98_RS03160 (ICU98_03155) dapD 597030..597857 (-) 828 WP_215352722.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48340.20 Da        Isoelectric Point: 7.6369

>NTDB_id=481655 ICU98_RS03145 WP_215352718.1 593582..594946(+) (radA) [Polynucleobacter sp. MWH-P3-07-1]
MAKAKTIYICQSCGGTSAKWQGQCPACQAWNTMEEGLPESSTPSRFQGLAQSLPRQKLAAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSAGMHVLYSSGEESAAQIALRAKRIALDAPALEVLAEIQLEKLLSIME
TVKPQVLVVDSIQTLYSEAFSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVSNPSAIFLSQHEQMVPGACVLVTQEGTRPLLVEIQALVDTAHIPNPR
RLAVGLEQHRLAMLLAVLHRHAGVACFDQDVFLNAVGGVKITEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFKLAIIPKANMPKNKIPGLKVIPVERIDQAIAAANELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481655 ICU98_RS03145 WP_215352718.1 593582..594946(+) (radA) [Polynucleobacter sp. MWH-P3-07-1]
TTGGCTAAAGCTAAAACGATTTATATCTGTCAGTCTTGTGGCGGCACTTCTGCCAAATGGCAAGGGCAGTGTCCTGCTTG
TCAGGCTTGGAACACCATGGAGGAGGGGCTTCCAGAGTCCAGCACGCCATCCCGTTTTCAGGGCTTAGCCCAATCCTTGC
CCAGACAAAAACTGGCGGCAATTACTGCGGAAGATTTGCCCCGCTTTAGCACGGGAGTTGAGGAGTTTGATCGGGTGCTC
GGCGGCGGCTTAGTTCCGGGTGGTGTCGTTTTGTTGGGCGGTGACCCTGGTATCGGTAAGTCAACTCTGTTACTCCAAGC
TTTGGCCGAGATGAGTTCGGCCGGCATGCATGTGCTGTATAGCAGTGGAGAAGAATCTGCTGCGCAAATTGCATTGCGGG
CTAAACGGATTGCTTTAGATGCGCCTGCGCTTGAAGTGCTGGCTGAGATTCAGTTGGAAAAACTATTATCAATTATGGAA
ACAGTGAAGCCTCAAGTGCTGGTAGTAGATTCCATTCAAACTCTGTATTCAGAAGCCTTCAGCTCTGCGCCAGGTTCGGT
TGCTCAGGTTCGCGAGTGTGCTGCGCAACTTACTAGAGCTGCTAAATCTAGTGGGATCTGCGTTTTGATGGTGGGGCACG
TGACGAAAGATGGCCATCTTGCTGGGCCTCGAGTCCTAGAGCACATTGTGGATACGGTTTTGTATTTTGAGGGAGATACC
CACTCTTCATTTAGATTGGTGCGCTCGATCAAGAATCGCTTTGGTGCCGTCAATGAACTCGGTGTTTTTGCGATGACTGA
AAAGGGTCTGCGGGGCGTATCGAATCCATCAGCGATTTTCTTGTCACAGCACGAGCAGATGGTGCCCGGCGCTTGTGTCT
TAGTGACTCAAGAAGGCACAAGACCGCTCTTGGTAGAGATTCAGGCGCTCGTCGATACTGCGCACATTCCTAATCCAAGA
AGATTGGCGGTGGGTCTAGAGCAGCATCGTTTGGCGATGTTGCTGGCAGTCTTGCATCGTCATGCTGGAGTCGCTTGTTT
TGATCAAGATGTATTTCTGAATGCAGTCGGCGGAGTCAAAATTACTGAGCCTGCTGCTGACTTGGCGGTATTGCTAGCCA
TTCAATCCTCCATTCGTAATCGCGCACTGCCAAAAGAGTTGATTGTCTTTGGCGAAGTAGGTTTAGCAGGAGAGATTCGT
CCTTGTCCTCGTGGTCAGGAGCGACTCAAGGAGGCTGCTAAATTAGGCTTTAAGTTGGCGATTATTCCAAAAGCCAATAT
GCCCAAGAATAAAATTCCAGGACTCAAGGTGATCCCTGTAGAGAGAATCGATCAGGCAATTGCTGCGGCGAATGAGCTTA
GTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-218)

Subunit ChlI of Mg-chelatase (ChlI)

(359-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.779

99.559

0.496
  radA Streptococcus pneumoniae Rx1

46

99.119

0.456
  radA Streptococcus pneumoniae D39

46

99.119

0.456
  radA Streptococcus pneumoniae R6

46

99.119

0.456
  radA Streptococcus pneumoniae TIGR4

46

99.119

0.456
  radA Streptococcus mitis NCTC 12261

46.911

96.256

0.452
  radA Streptococcus mitis SK321

46.911

96.256

0.452