Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ICV01_RS04450 Genome accession   NZ_CP061294
Coordinates   865970..867331 (+) Length   453 a.a.
NCBI ID   WP_215289008.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-Spelu-300-X4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 860970..872331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICV01_RS04425 (ICV01_04425) - 862727..863278 (-) 552 WP_215288990.1 peptidylprolyl isomerase -
  ICV01_RS04435 (ICV01_04435) - 863516..864376 (-) 861 WP_215288992.1 mechanosensitive ion channel family protein -
  ICV01_RS04440 (ICV01_04440) - 864422..865375 (+) 954 WP_215288993.1 MBL fold metallo-hydrolase -
  ICV01_RS04445 (ICV01_04445) - 865485..865970 (+) 486 WP_215289000.1 hypothetical protein -
  ICV01_RS04450 (ICV01_04450) radA 865970..867331 (+) 1362 WP_215289008.1 DNA repair protein RadA Machinery gene
  ICV01_RS04455 (ICV01_04455) prmB 867343..868224 (-) 882 WP_215289010.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  ICV01_RS04460 (ICV01_04460) dapE 868229..869386 (-) 1158 WP_215289014.1 succinyl-diaminopimelate desuccinylase -
  ICV01_RS04465 (ICV01_04465) dapD 869411..870244 (-) 834 WP_215289016.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 47955.72 Da        Isoelectric Point: 7.3467

>NTDB_id=481633 ICV01_RS04450 WP_215289008.1 865970..867331(+) (radA) [Polynucleobacter sp. MWH-Spelu-300-X4]
MAKVKSVYVCQSCGGMSAKWQGQCPNCDAWNTLEEAVEEKGSTSRFQSLAKAAPKQKLSVIEAEDLPRLSTGIDEFDRVL
GGGLVTGGVALIGGDPGIGKSTLLLQALAVMSARGDSVLYISGEESGSQIALRAKRLGIQAPDLDVLAEIQLEKLLVTIE
SSRPTVVVIDSIQTIYSEALTSAPGSVAQVKECAAQLTRLAKATGVCMIMVGHVTKEGNLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRAFKNRFGAVNELGVFAMTDKGLKGVANPSALFLSQHEQIVPGSCVLVTQEGSRPLLVEVQALVDTAHVPNPR
RLALGLEQHRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKISEPAADLAILLAIGSSLKNKALPKTLIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTLAIIPKANAPKVSIPGIKVIAVDRIDEAMDAARNL

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=481633 ICV01_RS04450 WP_215289008.1 865970..867331(+) (radA) [Polynucleobacter sp. MWH-Spelu-300-X4]
ATGGCTAAAGTTAAATCAGTTTATGTATGTCAATCTTGTGGTGGGATGTCAGCCAAATGGCAGGGGCAGTGCCCTAATTG
CGATGCTTGGAATACATTAGAAGAGGCGGTTGAAGAAAAGGGTTCGACAAGTAGATTTCAAAGTCTAGCCAAGGCTGCTC
CTAAACAAAAATTATCTGTTATTGAAGCTGAAGATCTACCACGCTTAAGTACTGGTATTGATGAGTTTGATCGTGTTCTA
GGTGGTGGTCTAGTTACTGGAGGAGTTGCTTTAATTGGCGGTGACCCTGGAATAGGAAAGTCAACCTTATTGCTTCAAGC
ACTTGCTGTGATGAGTGCCAGGGGCGATTCGGTTTTATATATAAGTGGCGAGGAATCAGGCTCACAAATTGCATTAAGAG
CTAAGCGTCTAGGTATTCAAGCGCCGGATCTAGATGTTCTAGCTGAGATACAGTTGGAGAAGTTATTGGTGACAATTGAA
TCAAGTAGGCCAACAGTGGTGGTAATTGACTCAATTCAAACGATTTATTCTGAAGCATTAACTTCTGCGCCAGGTTCAGT
CGCTCAAGTTAAAGAGTGTGCTGCTCAATTAACTAGGCTTGCCAAAGCTACAGGGGTTTGCATGATTATGGTTGGTCATG
TGACTAAAGAGGGCAACCTTGCTGGGCCAAGAGTGCTTGAGCATATTGTTGATACCGTCTTATATTTTGAAGGCGATACA
CATTCCTCGTTTAGATTGGTAAGAGCTTTCAAAAATCGTTTTGGTGCAGTTAATGAGCTTGGTGTTTTTGCCATGACTGA
TAAAGGACTTAAGGGTGTTGCTAACCCATCAGCCTTATTTTTATCTCAACATGAGCAGATAGTTCCCGGTTCTTGTGTGT
TAGTAACGCAAGAGGGGAGTCGTCCATTATTGGTTGAGGTTCAAGCACTTGTTGACACAGCTCATGTGCCCAATCCAAGG
CGCCTAGCATTAGGGCTTGAGCAGCATCGCTTGGCTATGTTGCTTGCTGTATTACATCGTCATGCTGGTATTGCATGCTT
TGACCAAGATGTTTTTTTGAATGCTGTTGGCGGTGTAAAAATTAGCGAGCCAGCAGCAGACTTGGCCATTCTATTGGCCA
TAGGCTCATCTTTAAAGAATAAAGCACTTCCCAAAACACTTATTGTCTTTGGTGAAGTTGGCTTGGCTGGGGAGATACGC
CCATGCCCAAGAGGTCAGGAGCGATTAAAGGAGGCAGCTAAGCTGGGCTTTACTTTAGCTATTATTCCTAAAGCTAATGC
GCCAAAAGTAAGTATCCCAGGTATTAAAGTTATTGCAGTCGATCGTATAGATGAGGCAATGGATGCCGCTCGGAATCTGT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-218)

Subunit ChlI of Mg-chelatase (ChlI)

(359-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.33

100

0.506
  radA Streptococcus pneumoniae Rx1

47.439

99.117

0.47
  radA Streptococcus pneumoniae D39

47.439

99.117

0.47
  radA Streptococcus pneumoniae R6

47.439

99.117

0.47
  radA Streptococcus pneumoniae TIGR4

47.439

99.117

0.47
  radA Streptococcus mitis NCTC 12261

47.439

99.117

0.47
  radA Streptococcus mitis SK321

47.439

99.117

0.47