Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ICV32_RS09355 Genome accession   NZ_CP061292
Coordinates   1792220..1793125 (+) Length   301 a.a.
NCBI ID   WP_215370398.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH24A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1787220..1798125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICV32_RS09325 (ICV32_09320) pth 1787394..1787984 (+) 591 WP_215370386.1 aminoacyl-tRNA hydrolase -
  ICV32_RS09330 (ICV32_09325) - 1787990..1788250 (-) 261 WP_215370389.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ICV32_RS09335 (ICV32_09330) coaD 1788283..1788774 (-) 492 WP_215370392.1 pantetheine-phosphate adenylyltransferase -
  ICV32_RS09340 (ICV32_09335) rsmD 1788809..1789399 (-) 591 WP_215370395.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ICV32_RS09345 (ICV32_09340) - 1789400..1790740 (-) 1341 WP_251371850.1 pitrilysin family protein -
  ICV32_RS09350 (ICV32_09345) - 1790752..1792170 (-) 1419 WP_251371851.1 pitrilysin family protein -
  ICV32_RS09355 (ICV32_09350) pilA 1792220..1793125 (+) 906 WP_215370398.1 signal recognition particle-docking protein FtsY Machinery gene
  ICV32_RS09360 (ICV32_09355) rpoH 1793259..1794149 (+) 891 WP_251371852.1 RNA polymerase sigma factor RpoH -
  ICV32_RS09365 (ICV32_09360) - 1794153..1794725 (-) 573 WP_215370403.1 SCO family protein -
  ICV32_RS09370 (ICV32_09365) cyoE 1794729..1795586 (-) 858 WP_215372703.1 heme o synthase -
  ICV32_RS09375 (ICV32_09370) - 1795613..1796737 (-) 1125 WP_251371853.1 heme A synthase -
  ICV32_RS09380 (ICV32_09375) - 1796734..1797387 (-) 654 WP_215370405.1 hypothetical protein -

Sequence

Protein


Download         Length: 301 a.a.        Molecular weight: 32551.27 Da        Isoelectric Point: 7.0730

>NTDB_id=481618 ICV32_RS09355 WP_215370398.1 1792220..1793125(+) (pilA) [Polynucleobacter sp. MWH-UH24A]
MFGLRKTLSSLFTRHRVDEAWFDHLEELLIKADVGVATSTFLITSLRKSAKEHAITNSEDLKAVLVSELSHHLSDLEPPE
NPLNPSAIHAKPEVWLIVGVNGAGKTTSIGKLCHHFQAQGKSVLLAAGDTFRAAARNQLKEWGERNQVQVLSQESGDAGA
IAHDAIQAALSRQVDIVLIDTAGRLATQTNLMEELKKVKRVISKLIPDAPHQMVLVLDGNTGQNGIAQVLAFRNAIGLHG
LIVTKLDGTAKGGVICALRNELENAPKNQGPKTYVYALGKGEQLGDLELFSAQAYAKELVE

Nucleotide


Download         Length: 906 bp        

>NTDB_id=481618 ICV32_RS09355 WP_215370398.1 1792220..1793125(+) (pilA) [Polynucleobacter sp. MWH-UH24A]
ATGTTTGGTTTGCGTAAAACACTTAGTTCACTTTTTACTCGTCACCGTGTTGATGAGGCCTGGTTTGACCATTTAGAAGA
ATTACTAATTAAAGCCGATGTTGGGGTTGCGACATCGACATTCCTAATCACTAGTCTACGCAAATCTGCAAAAGAACATG
CCATTACAAATTCTGAGGATTTAAAAGCCGTTCTTGTAAGTGAACTAAGCCATCACTTGAGCGACTTGGAGCCCCCTGAA
AACCCCCTAAATCCCTCTGCGATCCATGCAAAGCCTGAAGTTTGGCTGATCGTAGGGGTCAATGGTGCTGGCAAAACCAC
ATCAATTGGAAAACTATGTCACCACTTCCAAGCTCAAGGAAAATCGGTACTGCTTGCTGCGGGTGATACATTTCGAGCAG
CGGCTCGCAATCAACTCAAAGAATGGGGTGAGCGCAATCAAGTACAAGTACTTAGTCAAGAAAGCGGCGATGCGGGCGCT
ATTGCACATGATGCAATCCAGGCTGCGCTATCAAGGCAAGTGGATATCGTATTAATTGATACGGCTGGTCGACTGGCCAC
TCAGACTAACCTCATGGAAGAATTAAAGAAGGTTAAGCGAGTCATTTCGAAGTTGATTCCGGATGCCCCACATCAAATGG
TGTTGGTCCTTGATGGAAATACAGGACAAAATGGAATTGCCCAAGTTTTGGCCTTTCGGAACGCGATAGGACTGCACGGT
TTAATCGTTACTAAATTAGATGGCACCGCTAAGGGCGGGGTAATTTGTGCGCTTCGCAACGAATTAGAAAACGCCCCCAA
AAACCAGGGTCCTAAAACCTATGTCTACGCCCTTGGCAAGGGTGAGCAATTAGGTGATTTGGAGCTCTTTTCAGCCCAAG
CCTATGCTAAAGAGCTCGTAGAATAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-300)

SRP54-type protein, helical bundle domain (SRP54_N)

(3-69)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.829

99.336

0.485