Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ICV32_RS03035 Genome accession   NZ_CP061292
Coordinates   574394..575755 (+) Length   453 a.a.
NCBI ID   WP_215371820.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH24A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 569394..580755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICV32_RS03010 (ICV32_03005) - 569927..570355 (-) 429 WP_215371814.1 hypothetical protein -
  ICV32_RS03015 (ICV32_03010) - 570431..571666 (-) 1236 WP_215371815.1 hypothetical protein -
  ICV32_RS03025 (ICV32_03020) - 572057..573898 (+) 1842 WP_251371963.1 potassium transporter Kup -
  ICV32_RS03030 (ICV32_03025) - 573898..574392 (+) 495 WP_215371818.1 hypothetical protein -
  ICV32_RS03035 (ICV32_03030) radA 574394..575755 (+) 1362 WP_215371820.1 DNA repair protein RadA Machinery gene
  ICV32_RS03040 (ICV32_03035) prmB 575762..576631 (-) 870 WP_215372516.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  ICV32_RS03045 (ICV32_03040) dapE 576693..577841 (-) 1149 WP_215371821.1 succinyl-diaminopimelate desuccinylase -
  ICV32_RS03050 (ICV32_03045) dapD 577845..578672 (-) 828 WP_215371822.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48278.99 Da        Isoelectric Point: 7.1833

>NTDB_id=481609 ICV32_RS03035 WP_215371820.1 574394..575755(+) (radA) [Polynucleobacter sp. MWH-UH24A]
MAKTKTLYVCQACGGSSPKWQGQCPACDAWNTLEESLAETTNHRFQGLAKSIPRQRLVSIEAQDSPRLPTGIVELDRVLG
GGLVAGGVVLLGGDPGIGKSTLLLQALAELSMQGVDVLYSSGEESAAQIALRAQRISLNAPQLEILAEIQLEKLLLSVEA
AQPQVLVVDSIQTLYSEAFTSAPGSVAQVRECAAQLTRLAKSSGICILLVGHVTKDGHLAGPRVLEHIVDTVLYFEGDTH
SSFRLVRSIKNRFGAVNELGVFAMTEKGLKGVNNPSALFLSQHPQTVPGSCVLVTQEGSRPLLVEIQALVDTAHIPNPRR
LAVGLEQARLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAITSSIRNRALPKHLVVFGEVGLAGEIRP
CPRGQERLKEAAKLGFKIAIVPKANLPKSKITDLQMIAVERIDEAIAAANDLF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=481609 ICV32_RS03035 WP_215371820.1 574394..575755(+) (radA) [Polynucleobacter sp. MWH-UH24A]
ATGGCAAAGACCAAAACACTTTACGTTTGCCAGGCCTGTGGGGGCAGTTCTCCAAAATGGCAAGGTCAATGTCCTGCATG
CGATGCATGGAACACGCTTGAAGAGTCTCTCGCCGAGACTACAAACCATCGTTTTCAAGGGCTTGCCAAGTCAATCCCAC
GACAAAGATTGGTGAGCATTGAGGCACAGGATTCGCCACGATTACCAACCGGAATTGTTGAACTCGATCGCGTCTTAGGC
GGAGGATTGGTGGCAGGTGGTGTGGTCTTACTCGGCGGTGATCCAGGTATTGGGAAGTCCACCTTATTATTGCAAGCCTT
AGCTGAGCTAAGTATGCAAGGGGTCGATGTTTTATATAGCTCCGGAGAGGAGTCTGCCGCTCAAATTGCGTTGCGTGCTC
AGCGAATTTCCCTCAACGCACCTCAACTTGAAATCTTGGCAGAGATTCAGCTCGAAAAGTTATTGCTCTCGGTTGAGGCT
GCCCAACCGCAGGTTTTGGTGGTTGACTCGATTCAAACGCTCTATTCCGAGGCATTTACATCGGCGCCTGGATCGGTTGC
TCAAGTTCGAGAATGCGCCGCCCAATTAACGCGGCTAGCAAAATCAAGTGGGATCTGTATTTTATTGGTTGGCCACGTTA
CGAAAGATGGTCATCTGGCTGGTCCTCGTGTGCTGGAGCATATTGTGGATACCGTTTTGTATTTCGAGGGCGATACGCAT
TCTTCATTTCGTCTTGTGCGCTCAATTAAAAACCGCTTTGGGGCAGTTAACGAGCTTGGCGTCTTTGCGATGACCGAGAA
AGGTCTTAAAGGGGTTAATAATCCCTCTGCACTTTTTCTATCGCAACACCCGCAAACGGTACCTGGATCTTGTGTATTGG
TAACCCAAGAGGGTAGTCGACCGCTGTTGGTTGAGATTCAGGCGCTGGTTGATACGGCGCATATTCCGAATCCTAGACGC
CTAGCGGTTGGTTTAGAACAAGCGCGTTTGGCGATGCTGTTAGCGGTTTTGCACCGTCACGCAGGTATCGCTTGTTTTGA
TCAAGATGTTTTTCTCAATGCCGTGGGTGGGGTCAAAATTACCGAGCCCGCCGCTGATCTGGCAGTTCTACTGGCCATCA
CATCCTCAATCCGCAATCGGGCTCTACCAAAGCATTTGGTGGTCTTTGGTGAAGTTGGTTTGGCTGGAGAAATTCGTCCA
TGCCCTCGGGGTCAGGAACGATTGAAGGAGGCTGCTAAATTAGGATTTAAGATCGCCATTGTTCCTAAGGCTAATTTACC
AAAATCAAAAATTACTGATTTACAAATGATTGCTGTTGAGCGGATTGATGAGGCAATCGCTGCGGCGAACGACCTATTCT
GA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(358-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.231

100

0.494
  radA Streptococcus mitis NCTC 12261

46.137

100

0.461
  radA Streptococcus mitis SK321

46.341

99.558

0.461
  radA Streptococcus pneumoniae TIGR4

46.102

99.117

0.457
  radA Streptococcus pneumoniae R6

46.102

99.117

0.457
  radA Streptococcus pneumoniae Rx1

46.102

99.117

0.457
  radA Streptococcus pneumoniae D39

46.102

99.117

0.457