Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C2756_RS10095 Genome accession   NZ_CP061289
Coordinates   1950942..1951832 (+) Length   296 a.a.
NCBI ID   WP_215319117.1    Uniprot ID   -
Organism   Polynucleobacter sp. UB-Siik-W21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1945942..1956832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2756_RS10065 (C2756_10050) pth 1946153..1946743 (+) 591 WP_215319109.1 aminoacyl-tRNA hydrolase -
  C2756_RS10070 (C2756_10055) - 1946753..1947019 (-) 267 WP_215319111.1 YfhL family 4Fe-4S dicluster ferredoxin -
  C2756_RS10075 (C2756_10060) coaD 1947036..1947533 (-) 498 WP_215295913.1 pantetheine-phosphate adenylyltransferase -
  C2756_RS10080 (C2756_10065) rsmD 1947570..1948166 (-) 597 WP_215319113.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  C2756_RS10085 (C2756_10070) - 1948177..1949514 (-) 1338 WP_215319116.1 pitrilysin family protein -
  C2756_RS10090 (C2756_10075) - 1949514..1950878 (-) 1365 WP_215320956.1 pitrilysin family protein -
  C2756_RS10095 (C2756_10080) pilA 1950942..1951832 (+) 891 WP_215319117.1 signal recognition particle-docking protein FtsY Machinery gene
  C2756_RS10100 (C2756_10085) rpoH 1951925..1952854 (+) 930 WP_215319119.1 RNA polymerase sigma factor RpoH -
  C2756_RS10105 (C2756_10090) - 1952878..1953468 (-) 591 WP_215295919.1 SCO family protein -
  C2756_RS10110 (C2756_10095) cyoE 1953470..1954363 (-) 894 WP_215319128.1 heme o synthase -
  C2756_RS10115 (C2756_10100) - 1954360..1955463 (-) 1104 WP_215319131.1 heme A synthase -
  C2756_RS10120 (C2756_10105) - 1955463..1956119 (-) 657 WP_215319132.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31400.85 Da        Isoelectric Point: 6.6105

>NTDB_id=481595 C2756_RS10095 WP_215319117.1 1950942..1951832(+) (pilA) [Polynucleobacter sp. UB-Siik-W21]
MFGLRKTLGSLFKSSKTDEAWFDALEESLIQSDVGLPTTEQLIGKLRKAAKSEQASSPEELQALLIQEVGDLLRVLEPSP
NPLFSSQQDTIPEVWLVVGVNGAGKTTTIGKLCRRFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHSAISRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHTALGLTG
IIVTKLDGTAKGGVICALAHTLKDSPAPAVLALGKGEGIEDLVPFTAGQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481595 C2756_RS10095 WP_215319117.1 1950942..1951832(+) (pilA) [Polynucleobacter sp. UB-Siik-W21]
ATGTTCGGCTTACGTAAAACCCTCGGATCCCTATTCAAATCCAGCAAGACTGACGAAGCTTGGTTTGATGCCCTAGAAGA
ATCACTGATTCAAAGTGATGTGGGACTGCCGACGACTGAGCAATTGATCGGCAAACTGCGCAAAGCAGCAAAGTCTGAAC
AAGCATCCAGTCCAGAAGAACTTCAGGCACTACTCATTCAAGAGGTTGGAGATCTATTGCGGGTTTTAGAACCATCGCCG
AACCCTTTATTTTCAAGCCAGCAAGATACTATTCCAGAAGTTTGGTTGGTCGTTGGCGTTAATGGCGCAGGTAAAACTAC
CACCATTGGCAAACTTTGCAGGCGCTTTCAGTCTCAAGGTAAATCAGTACTCTTGGCTGCAGGTGACACTTTTAGAGCAG
CAGCGCGCAATCAACTACAGGAATGGGGTGGTCGCAACCAAGTTGATGTCATTACTCAAGAGGGGGGTGATGCAGCAGCA
GTTGCACATGATGCAATCCACTCAGCGATCTCTCGTAAAAGCGACATTCTGATTATTGATACTGCAGGACGCCTTGCCAC
CCAAGACCACCTCATGGAAGAGTTAAAAAAGGTGAAGAGGGTCATCGGCAAAGCCCTTCCTGGGGCCCCTCACCACACTC
TCTTGGTTTTAGATGGCAATACTGGTCAGAATGGCTTAAGCCAAGTGAAGGCATTTCATACTGCCCTGGGGCTCACTGGA
ATCATTGTTACCAAGCTCGATGGCACCGCTAAAGGCGGTGTTATCTGCGCCCTTGCCCACACCCTCAAAGATAGCCCCGC
ACCAGCAGTTTTGGCGCTTGGTAAAGGCGAGGGTATTGAAGATTTAGTCCCATTTACTGCCGGGCAATATTCTTCAGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-295)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.56

98.986

0.52