Detailed information NTG0481587

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: C2756_RS02695
• Genome accession: NZ_CP061289
• Coordinates: 505800..507164 (+)
• Length: 454 a.a.
• NCBI ID: WP_215319817.1
• Uniprot ID: -
• Organism: Polynucleobacter sp. UB-Siik-W21
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	493156..520000	505800..507164	within	0

Gene organization within MGE regions

Location: 493156..520000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2756_RS02640 (C2756_02640)	recJ	494307..496070 (-)	1764	WP_215319807.1	single-stranded-DNA-specific exonuclease RecJ	-
C2756_RS02645 (C2756_02645)	-	496067..497035 (-)	969	WP_251372106.1	hypothetical protein	-
C2756_RS02650 (C2756_02650)	-	497103..498365 (+)	1263	WP_215319811.1	lipoprotein-releasing ABC transporter permease subunit	-
C2756_RS02655 (C2756_02655)	-	498358..499053 (+)	696	WP_215319813.1	ABC transporter ATP-binding protein	-
C2756_RS02660 (C2756_02660)	-	499074..499910 (+)	837	WP_215320780.1	TatD family hydrolase	-
C2756_RS02665 (C2756_02665)	-	499975..502395 (+)	2421	WP_215319815.1	DNA internalization-related competence protein ComEC/Rec2	-
C2756_RS02670 (C2756_02670)	-	502443..502637 (+)	195	WP_215296503.1	type II toxin-antitoxin system HicA family toxin	-
C2756_RS02675 (C2756_02675)	-	502634..503026 (+)	393	WP_215296504.1	type II toxin-antitoxin system HicB family antitoxin	-
C2756_RS02685 (C2756_02685)	-	503417..505258 (+)	1842	WP_215320782.1	potassium transporter Kup	-
C2756_RS02690 (C2756_02690)	-	505255..505764 (+)	510	WP_215296508.1	hypothetical protein	-
C2756_RS02695 (C2756_02695)	radA	505800..507164 (+)	1365	WP_215319817.1	DNA repair protein RadA	Machinery gene
C2756_RS02700 (C2756_02700)	prmB	507188..508084 (-)	897	WP_215319819.1	50S ribosomal protein L3 N(5)-glutamine methyltransferase	-
C2756_RS02705 (C2756_02705)	dapE	508129..509280 (-)	1152	WP_215296511.1	succinyl-diaminopimelate desuccinylase	-
C2756_RS02710 (C2756_02710)	dapD	509287..510114 (-)	828	WP_215296512.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
C2756_RS02715 (C2756_02715)	smc	510290..513811 (+)	3522	WP_215319821.1	chromosome segregation protein SMC	-
C2756_RS02720 (C2756_02720)	-	513812..514876 (+)	1065	WP_251372107.1	cell division protein ZipA C-terminal FtsZ-binding domain-containing protein	-
C2756_RS02725 (C2756_02725)	ligA	514886..516901 (+)	2016	WP_215319823.1	NAD-dependent DNA ligase LigA	-
C2756_RS02730 (C2756_02730)	-	516914..517678 (+)	765	WP_215319825.1	class II aldolase/adducin family protein	-
C2756_RS02735 (C2756_02735)	-	517691..517876 (+)	186	WP_215296516.1	4-oxalocrotonate tautomerase	-
C2756_RS02740 (C2756_02740)	-	517995..519017 (+)	1023	WP_215319827.1	2-dehydropantoate 2-reductase	-
C2756_RS02745 (C2756_02745)	-	519077..520000 (-)	924	WP_215296518.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 454 a.a.
• Molecular weight: 48241.02 Da
• Isoelectric Point: 7.6029

>NTDB_id=481587 C2756_RS02695 WP_215319817.1 505800..507164(+) (radA) [Polynucleobacter sp. UB-Siik-W21]
MAKVKTVYICQSCGGTSAKWQGQCPSCQAWNTMEEGLPETTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSSGMNVLYSSGEESAAQIALRAKRIALNAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGGVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLKVIPVERIDQAIAAAAELS

Nucleotide

• Length: 1365 bp

>NTDB_id=481587 C2756_RS02695 WP_215319817.1 505800..507164(+) (radA) [Polynucleobacter sp. UB-Siik-W21]
TTGGCTAAAGTCAAAACGGTTTATATCTGCCAATCTTGTGGCGGTACTTCTGCAAAGTGGCAGGGCCAATGCCCATCTTG
TCAGGCGTGGAACACTATGGAAGAGGGCTTGCCCGAGACCACTTCTAACTCACGGTTTCAGGGTTTAGCGCAATCATTAC
CAAGACAAAAACTCTCTGCAATTACTGCCGAAGATTTGCCACGTTTTAGTACTGGTGTTGAAGAGTTTGATCGCGTGTTG
GGTGGTGGCTTGGTACCTGGTGGCGTAGTTTTATTGGGTGGCGATCCAGGAATTGGTAAATCAACACTCCTACTGCAAGC
TTTGGCTGAGATGAGCTCTTCAGGTATGAATGTACTCTATAGCAGTGGTGAAGAATCGGCTGCACAAATAGCATTGCGTG
CAAAACGCATCGCGCTCAATGCGCCACAACTAGAGGTCTTAGCAGAAATTCAGTTAGAAAAACTCATCTCCATCATGGAT
ACTGTCAAGCCACAGGTATTGGTGGTGGACTCTATTCAGACTTTGTATTCTGAGGTGTTAAGTTCAGCGCCAGGTTCCGT
CGCCCAAGTGCGTGAGTGCGCTGCCCAACTCACCAGAGCCGCAAAATCCAGTGGGATCTGTGTATTGATGGTGGGTCATG
TGACTAAAGATGGCCACTTGGCTGGCCCACGGGTGCTGGAGCACATTGTGGATACGGTATTGTATTTTGAGGGCGATACC
CATTCTTCATTTCGTTTAGTGCGCTCGATTAAGAATCGTTTTGGTGGCGTTAACGAGCTTGGTGTATTTGCAATGACTGA
AAAAGGTCTACGAGGGGTCACTAACCCGTCAGCAATCTTCTTATCGCAGCACGAGCAAATGGTTCCCGGCGCTTGCGTAT
TAGTCACTCAAGAGGGCAGTAGACCACTCTTGGTAGAAATTCAGGCATTGGTTGATACAGCACATGTTCCTAACCCACGG
CGTCTAGCAGTTGGCTTAGAGCAAGCCCGTTTAGCAATGCTCTTAGCGGTATTGCATCGCCATGCGGGCGTGGCGTGTTT
TGATCAAGATGTATTTTTAAATGCTGTTGGCGGTGTCAAGATTTCAGAACCAGCGGCTGACTTGGCAGTGCTGCTAGCAA
TTCAGTCATCGATTCGTAATCGCGCCTTACCTAAGGAGCTCATCGTCTTTGGTGAGGTAGGATTGGCTGGAGAGATTCGT
CCTTGTCCGCGCGGTCAAGAGCGTTTGAAAGAGGCTGCTAAGTTAGGCTTTACAGTGGCCATTATTCCAAAGGCGAATAT
GCCCAAGACCAAGATCCCGGGCTTAAAAGTAATTCCTGTAGAGCGAATTGATCAAGCGATCGCAGCTGCTGCAGAACTCA
GTTAA

Domains

Predicted by InterproScan.

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (357-450)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (78-218)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	50	99.559	0.498
radA	Streptococcus pneumoniae Rx1	45.778	99.119	0.454
radA	Streptococcus pneumoniae D39	45.778	99.119	0.454
radA	Streptococcus pneumoniae R6	45.778	99.119	0.454
radA	Streptococcus pneumoniae TIGR4	45.778	99.119	0.454
radA	Streptococcus mitis NCTC 12261	45.778	99.119	0.454
radA	Streptococcus mitis SK321	45.778	99.119	0.454