Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ICW03_RS10845 Genome accession   NZ_CP061287
Coordinates   2094074..2094964 (+) Length   296 a.a.
NCBI ID   WP_215348019.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-Aus1W21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2089074..2099964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICW03_RS10815 (ICW03_10810) pth 2089264..2089854 (+) 591 WP_215348014.1 aminoacyl-tRNA hydrolase -
  ICW03_RS10820 (ICW03_10815) - 2089872..2090138 (-) 267 WP_215348015.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ICW03_RS10825 (ICW03_10820) coaD 2090156..2090653 (-) 498 WP_215348016.1 pantetheine-phosphate adenylyltransferase -
  ICW03_RS10830 (ICW03_10825) rsmD 2090690..2091334 (-) 645 WP_256441444.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ICW03_RS10835 (ICW03_10830) - 2091309..2092646 (-) 1338 WP_215348017.1 pitrilysin family protein -
  ICW03_RS10840 (ICW03_10835) - 2092646..2094010 (-) 1365 WP_215348018.1 pitrilysin family protein -
  ICW03_RS10845 (ICW03_10840) pilA 2094074..2094964 (+) 891 WP_215348019.1 signal recognition particle-docking protein FtsY Machinery gene
  ICW03_RS10850 (ICW03_10845) rpoH 2095058..2095981 (+) 924 WP_215348020.1 RNA polymerase sigma factor RpoH -
  ICW03_RS10855 (ICW03_10850) - 2096015..2096599 (-) 585 WP_215348021.1 SCO family protein -
  ICW03_RS10860 (ICW03_10855) cyoE 2096607..2097500 (-) 894 WP_215348022.1 heme o synthase -
  ICW03_RS10865 (ICW03_10860) - 2097505..2098626 (-) 1122 WP_215348023.1 heme A synthase -
  ICW03_RS10870 (ICW03_10865) - 2098626..2099282 (-) 657 WP_215348024.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31305.78 Da        Isoelectric Point: 6.7584

>NTDB_id=481585 ICW03_RS10845 WP_215348019.1 2094074..2094964(+) (pilA) [Polynucleobacter sp. MWH-Aus1W21]
MFGLRKTLGSLFKSSKIDESWFDTLEESLIQSDVGLPTTEQLISKLRKAAKSEKASSPEELQALLIQEVSSLLTAIEPSP
NPLFTSDKPNTPEVWLVVGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHAAISRKNDILIIDTAGRLATQDNLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQEGPKPAVLALGKGEGIDDLAPFTAGQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481585 ICW03_RS10845 WP_215348019.1 2094074..2094964(+) (pilA) [Polynucleobacter sp. MWH-Aus1W21]
ATGTTCGGCTTACGTAAAACCCTCGGATCTCTTTTCAAATCAAGCAAGATTGATGAATCTTGGTTTGATACTTTGGAAGA
ATCACTAATACAAAGTGATGTGGGCCTTCCTACGACCGAACAACTCATCAGCAAACTACGTAAAGCAGCAAAATCAGAGA
AAGCCTCCAGCCCCGAAGAGTTACAAGCGCTACTTATTCAAGAAGTCAGCTCATTACTCACTGCTATTGAGCCTTCACCA
AATCCACTCTTTACTAGTGATAAGCCTAACACTCCAGAGGTATGGTTAGTTGTAGGGGTAAACGGTGCCGGCAAGACTAC
AACTATTGGCAAACTCTGCCGACTCTTTCAGTCTCAAGGTAAATCCGTTCTCCTGGCTGCTGGAGACACCTTCAGAGCCG
CTGCACGTAACCAGCTTCAAGAGTGGGGTGGACGCAATCAAGTGGATGTCATCACCCAGGAAGGTGGTGACGCTGCTGCC
GTTGCGCATGACGCAATTCATGCTGCTATCTCACGCAAGAATGACATTCTCATTATTGATACCGCAGGTCGTCTGGCAAC
CCAAGACAATCTGATGGAAGAACTCAAGAAGGTGAAACGTGTCATTGGCAAAGCACTCCCTGGAGCGCCGCACCACACAC
TACTTGTTTTAGACGGTAATACCGGGCAGAACGGCTTAAGCCAGGTAAAGGCATTTCATGCCGCCTTGGGACTGACTGGA
ATCATCGTGACCAAGCTTGACGGCACCGCTAAAGGCGGCGTGATCTGCGCTCTTGCCCACACCCTACAAGAAGGCCCAAA
ACCCGCTGTTCTAGCCCTAGGCAAAGGTGAGGGAATTGATGATTTAGCCCCCTTCACAGCAGGGCAATATTCTTCCGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-295)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.56

98.986

0.52