Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ICW03_RS02725 Genome accession   NZ_CP061287
Coordinates   514297..515661 (+) Length   454 a.a.
NCBI ID   WP_215348679.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-Aus1W21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 509297..520661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICW03_RS02715 (ICW03_02715) - 511916..513757 (+) 1842 WP_251374484.1 potassium transporter Kup -
  ICW03_RS02720 (ICW03_02720) - 513754..514263 (+) 510 WP_215348677.1 hypothetical protein -
  ICW03_RS02725 (ICW03_02725) radA 514297..515661 (+) 1365 WP_215348679.1 DNA repair protein RadA Machinery gene
  ICW03_RS02730 (ICW03_02730) prmB 515673..516569 (-) 897 WP_215348681.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  ICW03_RS02735 (ICW03_02735) dapE 516616..517767 (-) 1152 WP_215348683.1 succinyl-diaminopimelate desuccinylase -
  ICW03_RS02740 (ICW03_02740) dapD 517777..518604 (-) 828 WP_068322474.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48224.03 Da        Isoelectric Point: 7.3470

>NTDB_id=481577 ICW03_RS02725 WP_215348679.1 514297..515661(+) (radA) [Polynucleobacter sp. MWH-Aus1W21]
MAKVKTVYICQSCGGTSAKWQGQCPSCQAWNTMEEGLPETTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSAAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLKVIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=481577 ICW03_RS02725 WP_215348679.1 514297..515661(+) (radA) [Polynucleobacter sp. MWH-Aus1W21]
TTGGCTAAAGTCAAAACGGTTTATATCTGTCAGTCTTGTGGCGGTACCTCGGCCAAGTGGCAAGGTCAGTGCCCCTCTTG
TCAGGCCTGGAACACCATGGAAGAGGGTCTTCCAGAAACTACTTCTAATTCTCGTTTTCAGGGGTTGGCGCAATCATTGC
CAAGGCAAAAACTCTCCGCCATTACAGCTGAAGACTTACCAAGATTTAGCACGGGCGTAGAAGAGTTTGATCGCGTGCTG
GGTGGCGGCTTGGTTCCAGGCGGTGTAGTGCTCTTGGGTGGTGATCCTGGTATTGGTAAATCCACTCTACTGTTGCAGGC
ACTTGCAGAGATGAGTGCTGCCGGCATGAATGTCCTCTATAGCAGCGGTGAAGAATCGGCTGCACAGATTGCCTTACGTG
CAAAACGTATTGCATTGGATGCACCTCAACTAGAAGTATTGGCTGAAATTCAGTTGGAGAAACTGATTTCCATTATGGAT
ACCGTGAAGCCGCAAGTATTGGTGGTGGATTCCATCCAGACTTTGTACTCTGAGGTTTTGAGTTCAGCTCCTGGTTCTGT
TGCGCAAGTTCGTGAGTGTGCGGCGCAACTCACTCGAGCTGCAAAATCGAGCGGTATTTGTGTATTGATGGTTGGTCACG
TGACTAAGGATGGCCATCTCGCTGGCCCAAGAGTTTTAGAACATATCGTAGACACCGTGTTGTATTTTGAGGGCGATACC
CATTCTTCCTTCCGCTTAGTGCGTTCGATCAAGAACCGTTTTGGCGCTGTTAATGAGTTGGGTGTCTTTGCCATGACAGA
GAAAGGTTTGCGCGGCGTTACTAACCCGTCTGCCATTTTCTTGTCGCAGCATGAGCAAATGGTCCCCGGTGCCTGCGTAC
TAGTGACTCAAGAGGGTAGTAGACCCTTATTGGTGGAGATACAGGCTTTGGTGGATACAGCGCATGTACCCAATCCTCGA
CGTTTAGCTGTTGGTCTAGAGCAGGCCCGCTTAGCCATGCTCTTGGCTGTGTTACATCGTCATGCTGGTGTTGCTTGCTT
TGATCAAGATGTTTTCTTAAATGCGGTCGGTGGCGTCAAAATTTCTGAGCCAGCTGCTGACCTAGCCGTTCTCCTGGCTA
TTCAGTCATCGATCCGAAATCGCGCTTTACCAAAAGAATTAATTGTCTTTGGTGAGGTTGGTTTAGCTGGAGAAATTCGT
CCCTGTCCGCGTGGTCAGGAACGTTTGAAGGAGGCTGCTAAATTGGGCTTCACTGTAGCCATCATTCCAAAAGCCAATAT
GCCTAAGACTAAGATTCCGGGGTTGAAGGTGATTCCGGTCGAGCGAATTGATCAAGCAATCGCGGCAGCAGCAGAGTTGA
GCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(78-218)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.558

99.559

0.493
  radA Streptococcus pneumoniae Rx1

45.778

99.119

0.454
  radA Streptococcus pneumoniae D39

45.778

99.119

0.454
  radA Streptococcus pneumoniae R6

45.778

99.119

0.454
  radA Streptococcus pneumoniae TIGR4

45.778

99.119

0.454
  radA Streptococcus mitis NCTC 12261

45.778

99.119

0.454
  radA Streptococcus mitis SK321

45.778

99.119

0.454