Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ICV36_RS09480 Genome accession   NZ_CP061285
Coordinates   1830453..1831343 (+) Length   296 a.a.
NCBI ID   WP_215400436.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH35A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1825453..1836343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICV36_RS09450 (ICV36_09450) pth 1825645..1826235 (+) 591 WP_215400431.1 aminoacyl-tRNA hydrolase -
  ICV36_RS09455 (ICV36_09455) - 1826245..1826511 (-) 267 WP_215400432.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ICV36_RS09460 (ICV36_09460) coaD 1826535..1827032 (-) 498 WP_215400433.1 pantetheine-phosphate adenylyltransferase -
  ICV36_RS09465 (ICV36_09465) rsmD 1827069..1827716 (-) 648 WP_371743201.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ICV36_RS09470 (ICV36_09470) - 1827688..1829025 (-) 1338 WP_215400434.1 pitrilysin family protein -
  ICV36_RS09475 (ICV36_09475) - 1829025..1830389 (-) 1365 WP_215400435.1 pitrilysin family protein -
  ICV36_RS09480 (ICV36_09480) pilA 1830453..1831343 (+) 891 WP_215400436.1 signal recognition particle-docking protein FtsY Machinery gene
  ICV36_RS09485 (ICV36_09485) rpoH 1831437..1832360 (+) 924 WP_215400437.1 RNA polymerase sigma factor RpoH -
  ICV36_RS09490 (ICV36_09490) - 1832405..1832989 (-) 585 WP_215400438.1 SCO family protein -
  ICV36_RS09495 (ICV36_09495) cyoE 1833020..1833913 (-) 894 WP_215400439.1 heme o synthase -
  ICV36_RS09500 (ICV36_09500) - 1833916..1835037 (-) 1122 WP_251375003.1 heme A synthase -
  ICV36_RS09505 (ICV36_09505) - 1835037..1835693 (-) 657 WP_215400440.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31275.76 Da        Isoelectric Point: 6.7582

>NTDB_id=481575 ICV36_RS09480 WP_215400436.1 1830453..1831343(+) (pilA) [Polynucleobacter sp. MWH-UH35A]
MFGLRKTLGSLFKSSKIDESWFDTLEESLIQSDVGLPTTEQLISKLRKAAKSEKASSPEELQALLIQEVSALLTAIEPSP
NPLFTSDKPNTPEVWLVVGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHAAISRKNDILIIDTAGRLATQDNLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQDGPKPAVLALGKGEGIDDLAPFTAGQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=481575 ICV36_RS09480 WP_215400436.1 1830453..1831343(+) (pilA) [Polynucleobacter sp. MWH-UH35A]
ATGTTCGGCCTACGTAAAACCCTTGGATCTCTTTTTAAATCAAGCAAGATTGATGAATCTTGGTTTGATACTCTAGAAGA
ATCACTTATACAAAGTGATGTTGGCCTACCCACGACCGAACAACTCATTAGCAAGCTTCGTAAAGCAGCGAAATCCGAAA
AGGCATCCAGTCCTGAAGAGTTACAAGCCTTATTGATTCAAGAGGTCAGTGCACTGTTGACTGCTATTGAACCTTCACCA
AACCCGCTATTTACAAGTGACAAGCCTAACACCCCGGAGGTATGGCTGGTTGTAGGGGTAAATGGGGCTGGTAAGACAAC
AACCATTGGCAAGCTTTGCAGACTCTTTCAGTCGCAAGGTAAGTCAGTTCTCCTAGCGGCAGGCGATACCTTCAGGGCAG
CTGCACGCAATCAACTCCAGGAATGGGGTGGACGCAACCAAGTGGATGTCATTACCCAAGAAGGTGGTGATGCGGCTGCC
GTTGCCCATGATGCAATTCATGCGGCCATCTCCCGCAAGAACGACATTCTGATTATTGACACTGCGGGTCGCCTAGCGAC
TCAAGATAACTTGATGGAAGAGCTTAAGAAAGTGAAGCGAGTTATCGGCAAAGCACTTCCTGGAGCACCACACCACACAC
TACTAGTGCTAGATGGCAATACGGGGCAGAACGGCCTAAGCCAGGTCAAAGCCTTTCATGCCGCTCTCGGCCTTACTGGA
ATCATCGTGACCAAGCTAGATGGCACCGCAAAGGGCGGGGTGATCTGCGCCCTAGCCCATACCCTACAAGACGGACCAAA
ACCCGCTGTGTTAGCCCTGGGCAAAGGGGAAGGTATTGATGATTTGGCCCCCTTTACAGCAGGGCAATATTCATCCGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-295)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.242

98.986

0.527