Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   IAU66_RS12260 Genome accession   NZ_CP061168
Coordinates   2555449..2556249 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus amyloliquefaciens strain T-5     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2550449..2561249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAU66_RS12230 - 2550996..2551628 (+) 633 WP_014305389.1 helix-turn-helix domain-containing protein -
  IAU66_RS12235 - 2551919..2552056 (+) 138 WP_014470646.1 hypothetical protein -
  IAU66_RS12240 - 2552150..2553268 (+) 1119 WP_024085589.1 NADH-dependent flavin oxidoreductase -
  IAU66_RS12245 - 2553394..2553606 (+) 213 WP_024085590.1 spore germination protein -
  IAU66_RS12250 - 2553732..2553944 (+) 213 WP_014305392.1 spore germination protein -
  IAU66_RS12255 alr 2554120..2555301 (+) 1182 WP_014305393.1 alanine racemase -
  IAU66_RS12260 spo0A 2555449..2556249 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  IAU66_RS12265 spoIVB 2556530..2557810 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  IAU66_RS12270 recN 2557973..2559703 (-) 1731 WP_024085591.1 DNA repair protein RecN Machinery gene
  IAU66_RS12275 ahrC 2559735..2560184 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  IAU66_RS12280 - 2560281..2561126 (-) 846 WP_015388017.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=481071 IAU66_RS12260 WP_003153177.1 2555449..2556249(-) (spo0A) [Bacillus amyloliquefaciens strain T-5]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=481071 IAU66_RS12260 WP_003153177.1 2555449..2556249(-) (spo0A) [Bacillus amyloliquefaciens strain T-5]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTTGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97