Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   IAU66_RS08400 Genome accession   NZ_CP061168
Coordinates   1681901..1682800 (+) Length   299 a.a.
NCBI ID   WP_015388238.1    Uniprot ID   A0A6A8LH26
Organism   Bacillus amyloliquefaciens strain T-5     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1676901..1687800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAU66_RS08380 - 1677592..1679295 (+) 1704 WP_024085298.1 hypothetical protein -
  IAU66_RS08385 - 1679292..1679573 (+) 282 WP_003154284.1 FlhB-like flagellar biosynthesis protein -
  IAU66_RS08390 sucC 1679748..1680905 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  IAU66_RS08395 sucD 1680934..1681836 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  IAU66_RS08400 dprA 1681901..1682800 (+) 900 WP_015388238.1 DNA-processing protein DprA Machinery gene
  IAU66_RS08405 topA 1682982..1685057 (+) 2076 WP_003154274.1 type I DNA topoisomerase -
  IAU66_RS08410 trmFO 1685122..1686429 (+) 1308 WP_003154273.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  IAU66_RS08415 xerC 1686499..1687416 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32820.92 Da        Isoelectric Point: 7.8285

>NTDB_id=481065 IAU66_RS08400 WP_015388238.1 1681901..1682800(+) (dprA) [Bacillus amyloliquefaciens strain T-5]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKDPELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTPLLEKGRKIGIVGTRKPTEDGIKAAGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=481065 IAU66_RS08400 WP_015388238.1 1681901..1682800(+) (dprA) [Bacillus amyloliquefaciens strain T-5]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCATCAAGCCGCTACCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGAAACAC
ACCGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGCTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGTTTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LH26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.569

100

0.706
  dprA Lactococcus lactis subsp. cremoris KW2

41.288

88.294

0.365