Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J5N95_RS15865 Genome accession   NZ_CP072054
Coordinates   3316391..3317068 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain K1508     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311391..3322068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5N95_RS15850 (J5N95_15850) chiA 3312758..3315451 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  J5N95_RS15855 (J5N95_15855) bfd 3315620..3315814 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J5N95_RS15860 (J5N95_15860) bfr 3315886..3316362 (+) 477 WP_000675504.1 bacterioferritin -
  J5N95_RS15865 (J5N95_15865) pilD 3316391..3317068 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  J5N95_RS15870 (J5N95_15870) gspM 3317068..3317529 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  J5N95_RS15875 (J5N95_15875) gspL 3317526..3318689 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  J5N95_RS15880 (J5N95_15880) gspK 3318704..3319687 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  J5N95_RS15885 (J5N95_15885) gspJ 3319680..3320267 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  J5N95_RS15890 (J5N95_15890) gspI 3320260..3320637 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J5N95_RS15895 (J5N95_15895) gspH 3320634..3321143 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  J5N95_RS15900 (J5N95_15900) gspG 3321151..3321588 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=480475 J5N95_RS15865 WP_000178154.1 3316391..3317068(-) (pilD) [Escherichia coli strain K1508]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=480475 J5N95_RS15865 WP_000178154.1 3316391..3317068(-) (pilD) [Escherichia coli strain K1508]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378