Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   J4A01_RS10030 Genome accession   NZ_CP071932
Coordinates   2095942..2097078 (+) Length   378 a.a.
NCBI ID   WP_032873352.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain CAS02     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2090942..2102078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4A01_RS09995 (J4A01_09895) - 2092351..2092776 (+) 426 WP_032873355.1 SDR family NAD(P)-dependent oxidoreductase -
  J4A01_RS10000 (J4A01_09900) sprB 2092810..2092986 (-) 177 WP_007612049.1 recombination directionality factor SprB -
  J4A01_RS10005 (J4A01_09905) - 2092989..2093161 (-) 173 Protein_1915 hypothetical protein -
  J4A01_RS10010 (J4A01_09910) - 2093349..2093705 (-) 357 WP_032873513.1 DUF3221 domain-containing protein -
  J4A01_RS10015 (J4A01_09915) - 2094154..2094390 (+) 237 WP_007407922.1 hypothetical protein -
  J4A01_RS10020 (J4A01_09920) - 2094516..2094890 (+) 375 WP_007407921.1 YoqO family protein -
  J4A01_RS10025 (J4A01_09925) - 2095122..2095573 (-) 452 Protein_1919 hypothetical protein -
  J4A01_RS10030 (J4A01_09930) rapF 2095942..2097078 (+) 1137 WP_032873352.1 Rap family tetratricopeptide repeat protein Regulator
  J4A01_RS10035 (J4A01_09935) - 2097068..2097250 (+) 183 WP_003153655.1 hypothetical protein -
  J4A01_RS10040 (J4A01_09940) - 2097559..2098578 (+) 1020 WP_032873350.1 hypothetical protein -
  J4A01_RS10045 (J4A01_09945) - 2098639..2098998 (-) 360 WP_032873347.1 hypothetical protein -
  J4A01_RS10050 (J4A01_09950) - 2099404..2099493 (+) 90 WP_108017291.1 putative holin-like toxin -
  J4A01_RS10055 (J4A01_09955) - 2099748..2100206 (-) 459 WP_032873346.1 SMI1/KNR4 family protein -
  J4A01_RS10060 (J4A01_09960) - 2100222..2102024 (-) 1803 WP_032873344.1 T7SS effector LXG polymorphic toxin -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 44658.20 Da        Isoelectric Point: 4.7141

>NTDB_id=479610 J4A01_RS10030 WP_032873352.1 2095942..2097078(+) (rapF) [Bacillus amyloliquefaciens strain CAS02]
MEQLISSSKVGLKINEWYKYIRLFSVADAEILKEEVKQEIELMEEDQDLLLYYSLMCFRHQLMLDYLEPKTMNEERPEIS
DLSEEIESKQSDLKGILEYYFNFFHGMYEFEQYEYLKAISFYNRAEKKISIVPDEIERAEFHYKVAEVYYHMKQTHMSMH
HIVQAIETYKAHETYSIRVIQCSFVIGLNYLDMEYPEKAIPHFMNALKQAQEIDMPRLIGSSLYNLGLCSFTTGHMEKAL
EYFKEGVSIYQTNSLHNSSRLLDILLMLAKTHFKLSNTVDGKQVCEQGLMLSKDLNDDVLEKMFDFLMGLFVENDKERLF
KILDYLESKAMLSDVEDLATDAAMYYNVNEDHKVATAFYEKVLYARKQIQRGDCLYEI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=479610 J4A01_RS10030 WP_032873352.1 2095942..2097078(+) (rapF) [Bacillus amyloliquefaciens strain CAS02]
TTGGAGCAATTGATCTCTTCTTCAAAAGTTGGCTTAAAAATCAATGAATGGTACAAATACATAAGGTTATTCAGTGTTGC
AGATGCAGAAATCTTGAAGGAAGAAGTGAAGCAAGAAATAGAGCTTATGGAAGAAGATCAAGATTTATTGTTGTACTATT
CGCTCATGTGTTTTCGACATCAATTAATGCTTGATTATCTTGAACCAAAAACCATGAATGAAGAACGACCTGAAATTTCA
GACTTATCAGAAGAGATCGAAAGTAAACAAAGCGATCTAAAAGGAATTCTTGAATATTATTTTAACTTTTTTCATGGGAT
GTATGAATTTGAACAGTACGAATATCTTAAAGCAATAAGTTTTTACAACCGAGCTGAAAAGAAGATATCTATTGTCCCCG
ATGAAATAGAGCGAGCTGAATTTCACTATAAAGTTGCTGAAGTGTACTATCACATGAAGCAAACCCACATGTCTATGCAT
CATATCGTTCAAGCAATAGAAACCTACAAAGCACACGAAACGTACTCAATAAGAGTAATCCAATGTTCATTCGTCATCGG
TCTGAATTATTTAGACATGGAGTATCCAGAAAAAGCAATACCGCATTTCATGAATGCATTAAAACAAGCACAAGAAATTG
ATATGCCACGGTTAATAGGTTCTTCTTTGTACAATCTCGGTTTATGCTCATTTACAACAGGACATATGGAGAAGGCATTG
GAGTATTTTAAAGAAGGCGTCTCAATTTATCAAACTAATTCTCTTCACAATTCTAGCCGACTGCTTGACATACTTTTAAT
GCTGGCAAAAACGCACTTTAAGTTGTCCAACACAGTTGATGGAAAACAAGTTTGTGAACAAGGTCTTATGCTGTCAAAGG
ATTTGAACGATGACGTATTAGAGAAAATGTTTGATTTTCTTATGGGTTTATTCGTTGAGAATGACAAAGAACGACTTTTT
AAAATCTTAGATTACTTAGAATCAAAAGCTATGCTTTCAGATGTGGAAGACCTAGCGACTGATGCAGCAATGTATTATAA
TGTAAATGAAGACCATAAAGTAGCTACAGCCTTTTATGAGAAAGTGCTTTATGCCCGAAAACAAATTCAGAGAGGGGATT
GTCTTTATGAAATTTAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(222-252)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

45.932

100

0.463
  rapC Bacillus subtilis subsp. subtilis str. 168

45.503

100

0.455