Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IBC33_RS01945 Genome accession   NZ_CP061006
Coordinates   380944..382320 (+) Length   458 a.a.
NCBI ID   WP_005720413.1    Uniprot ID   A0A2A7RGN6
Organism   Lactobacillus crispatus strain lc31     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 375944..387320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IBC33_RS01910 - 375996..376178 (+) 183 WP_005720404.1 hypothetical protein -
  IBC33_RS01915 - 376190..376870 (+) 681 WP_126708790.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  IBC33_RS01920 - 376981..377916 (+) 936 WP_005724201.1 AAA family ATPase -
  IBC33_RS01925 - 377980..378384 (-) 405 WP_005725567.1 hypothetical protein -
  IBC33_RS01930 pepC 378544..379893 (-) 1350 WP_005725566.1 aminopeptidase C -
  IBC33_RS01935 - 379962..380261 (-) 300 WP_005725564.1 hypothetical protein -
  IBC33_RS01940 - 380393..380944 (+) 552 WP_005720412.1 dUTP diphosphatase -
  IBC33_RS01945 radA 380944..382320 (+) 1377 WP_005720413.1 DNA repair protein RadA Machinery gene
  IBC33_RS01950 gltX 382398..383897 (+) 1500 WP_005726795.1 glutamate--tRNA ligase -
  IBC33_RS01955 cysS 383988..385421 (+) 1434 WP_005724208.1 cysteine--tRNA ligase -
  IBC33_RS01960 - 385414..385857 (+) 444 WP_005726794.1 mini-ribonuclease III -
  IBC33_RS01965 rlmB 385844..386599 (+) 756 WP_005724210.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IBC33_RS01970 - 386733..387278 (+) 546 WP_005724212.1 DNA-directed RNA polymerase subunit sigma -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50214.83 Da        Isoelectric Point: 7.1405

>NTDB_id=479415 IBC33_RS01945 WP_005720413.1 380944..382320(+) (radA) [Lactobacillus crispatus strain lc31]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVKLDKIKAEKEERIVTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFIPRHNMYSGLKDHGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=479415 IBC33_RS01945 WP_005720413.1 380944..382320(+) (radA) [Lactobacillus crispatus strain lc31]
ATGGCTAGAGTAAAAACACAATATAAGTGTCGTTCTTGCGGCTATATTTCGGCAAGCTATTTAGGCCGCTGCCCTAACTG
TGGTGCTTGGAATCAATTTGAAAAAGAAACTGAAGAAGTGCAAAAGCGTTCTACTAAGGCAACTGCTAGTCGTTTGATTC
AGAAGACTGGATTAAATGAACCGGTAAAGCTGGATAAGATTAAAGCTGAAAAAGAAGAACGAATTGTCACTAAATCTGAG
GAATTAAACCGCGTTTTAGGTGGTGGTATTGTGCCAGGCTCACTAGTTTTAATTGGTGGAGATCCCGGAATTGGTAAGTC
AACCTTGATGTTACAAATTATGAGTGACTTATCTGAAAAATATAAGGTGCTTTACGTTTCGGGAGAAGAATCAGCTAACC
AAATCAAATTGCGGGCTGACCGACTGGGTGTAGGTCAAAGTAATATGCTCTTATATCCAGAGACTGACATGCATGATATT
CGCGAACAAATCAATGATGTTAAGCCTGACTTTGTTGTAATCGACTCCATTCAAACTATGAATGAACCAAGTCTTGATTC
GATGACTGGCTCTGCCTCACAGGTTCGTGAAGTAACAAGTGAATTAATGAAAATTGCCAAGATGGATGCCATTACAGTTT
TTGTTATCGGCCACGTTACAAAAGAGGGTGCGATTGCTGGGCCTAAGATCTTGGAACATATGGTGGATACCGTTCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGAATTTTGCATTCTGTTAAAAACCGTTTTGGAGCAGCAAACGAAATTGGAAT
GTTTGAAATGGTTAATGAAGGCTTAAGAGAGGTAACTAATCCATCATCAATTTTTCTGGACCAAAGATTACCTAATTCTA
CTGGCTCTGCTGTGGTGGTATCGCTTGAAGGAACTAGGCCACTTTTAGCAGAAATTCAGGCTTTGGTTACTCCGACAGCT
TTTGGCTATGCTAAAAGGACCACGTCAGGTATTAGTTTTAACAAAGCTTCATTGCTGCTAGCGGTGCTTGAAAAGCGGGG
AAACTTAATGTTGCAGAATCAAGATGTCTATTTGACTGCAACTGGTGGGATTAAATTGAATGAACCAGCAATTGATTTAG
CAATTGCCATGGCAGTTGCATCCAGTTATACCGATAAAGAAATTTCGCCGACAGATTGTTTTGTAGGCGAAGTGGGCTTA
ACTGGTGAAGTTCGCCGAGTTGATAAAATCGATGCACGAGTAAAAGAAGCGGCTAAAGTAGGGTTTAAGCGGATCTTTAT
TCCACGGCATAATATGTATTCTGGATTAAAAGATCATGGCATTGAGGTAATTCCCGTTTCGAGTATTCCCCAAGCTTTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A7RGN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.773

100

0.559