Detailed information NTG0479400

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: H8687_RS01895
• Genome accession: NZ_CP061005
• Coordinates: 367147..368523 (+)
• Length: 458 a.a.
• NCBI ID: WP_005724205.1
• Uniprot ID: -
• Organism: Lactobacillus crispatus strain lc83
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	365561..366418	367147..368523	flank	729

Gene organization within MGE regions

Location: 365561..368523

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H8687_RS01885	-	365561..366418 (-)	858	WP_118992163.1	IS982 family transposase	-
H8687_RS01890	-	366596..367147 (+)	552	WP_005720412.1	dUTP diphosphatase	-
H8687_RS01895	radA	367147..368523 (+)	1377	WP_005724205.1	DNA repair protein RadA	Machinery gene

Sequence

Protein

• Length: 458 a.a.
• Molecular weight: 50230.87 Da
• Isoelectric Point: 7.1405

>NTDB_id=479400 H8687_RS01895 WP_005724205.1 367147..368523(+) (radA) [Lactobacillus crispatus strain lc83]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVKLDKIKAEKEERIVTKSE
ELNRVLGGGIVLGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFIPRHNMYSGLKDHGIEVIPVSSIPQALKLVFG

Nucleotide

• Length: 1377 bp

>NTDB_id=479400 H8687_RS01895 WP_005724205.1 367147..368523(+) (radA) [Lactobacillus crispatus strain lc83]
ATGGCTAGAGTAAAAACACAATATAAGTGTCGTTCTTGCGGCTATATTTCGGCAAGCTATTTAGGCCGCTGCCCTAACTG
TGGTGCTTGGAATCAATTTGAAAAAGAAACTGAAGAAGTGCAAAAGCGTTCTACTAAGGCAACTGCTAGTCGTTTGATTC
AGAAGACTGGATTAAATGAACCGGTAAAGCTGGATAAGATTAAAGCTGAAAAAGAAGAACGAATTGTCACTAAATCTGAG
GAATTAAATCGCGTTTTAGGTGGTGGTATTGTGCTAGGCTCACTAGTTTTAATTGGTGGAGATCCCGGAATTGGTAAGTC
AACCTTGATGTTACAAATTATGAGTGACTTATCTGAAAAATATAAGGTGCTTTACGTTTCGGGAGAAGAATCAGCTAACC
AAATCAAATTGCGGGCTGACCGACTGGGTGTAGGTCAAAGTAATATGCTCTTATATCCAGAGACTGACATGCATGATATT
CGCGAACAAATCAATGATGTTAAGCCTGACTTTGTTGTAATCGACTCCATTCAAACTATGAATGAACCAAGTCTTGATTC
GATGACTGGCTCTGCCTCACAGGTTCGTGAAGTAACAAGTGAATTAATGAAAATTGCCAAGATGGATGCCATTACAGTTT
TTGTTATCGGCCACGTTACAAAAGAGGGTGCGATTGCTGGGCCTAAGATCTTGGAACATATGGTGGATACCGTTCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGAATTTTGCATTCTGTTAAAAACCGTTTTGGAGCAGCAAACGAAATTGGAAT
GTTTGAAATGGTTAATGAAGGCTTAAGAGAGGTAACTAATCCATCATCAATTTTTCTGGACCAAAGATTACCTAATTCTA
CTGGCTCTGCTGTGGTGGTATCGCTTGAAGGAACTAGGCCACTTTTAGCAGAAATTCAGGCTTTGGTTACTCCGACAGCT
TTTGGCTATGCTAAAAGGACCACGTCAGGTATTAGTTTTAACAAAGCTTCATTGCTGCTAGCGGTGCTTGAAAAGCGGGG
AAACTTAATGTTGCAGAATCAAGATGTCTATTTGACTGCAACTGGTGGGATTAAATTGAATGAACCAGCAATTGATTTAG
CAATTGCCATGGCAGTTGCATCCAGTTATACCGATAAAGAAATTTCGCCGACAGATTGTTTTGTAGGCGAAGTGGGCTTA
ACTGGTGAAGTTCGCCGAGTTGATAAAATCGATGCACGAGTAAAAGAAGCGGCTAAAGTAGGGTTTAAGCGGATCTTTAT
TCCACGGCATAATATGTATTCTGGATTAAAAGATCATGGCATTGAGGTAATTCCCGTTTCGAGTATTCCCCAAGCTTTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (66-223)

• Subunit ChlI of Mg-chelatase (ChlI) (363-431)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-34)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus mitis SK321	57.33	99.782	0.572
radA	Streptococcus mitis NCTC 12261	57.33	99.782	0.572
radA	Streptococcus pneumoniae Rx1	57.33	99.782	0.572
radA	Streptococcus pneumoniae D39	57.33	99.782	0.572
radA	Streptococcus pneumoniae R6	57.33	99.782	0.572
radA	Streptococcus pneumoniae TIGR4	57.33	99.782	0.572
radA	Bacillus subtilis subsp. subtilis str. 168	55.773	100	0.559