Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JN057_RS00425 Genome accession   NZ_CP071871
Coordinates   61252..62613 (+) Length   453 a.a.
NCBI ID   WP_078065389.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain BVJ1JL     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 56252..67613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN057_RS00370 (JN057_00078) - 56325..56528 (+) 204 WP_001091109.1 hypothetical protein -
  JN057_RS00375 (JN057_00079) - 56531..56881 (+) 351 WP_000852247.1 hypothetical protein -
  JN057_RS00380 (JN057_00080) - 56891..57307 (+) 417 WP_001165344.1 phage holin family protein -
  JN057_RS00385 (JN057_00081) - 57311..57646 (+) 336 WP_001186237.1 phage holin -
  JN057_RS00390 (JN057_00082) - 57646..58602 (+) 957 WP_000350458.1 N-acetylmuramoyl-L-alanine amidase family protein -
  JN057_RS00395 (JN057_00083) - 58872..59051 (-) 180 WP_001209433.1 hypothetical protein -
  JN057_RS00400 (JN057_00084) - 59193..59342 (-) 150 WP_001030863.1 hypothetical protein -
  JN057_RS00405 (JN057_00085) tadA 59624..60091 (+) 468 WP_000291875.1 tRNA adenosine(34) deaminase TadA -
  JN057_RS00415 (JN057_00087) - 60278..60721 (+) 444 WP_000701992.1 dUTP diphosphatase -
  JN057_RS00420 (JN057_00088) - 60723..61238 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  JN057_RS00425 (JN057_00089) radA 61252..62613 (+) 1362 WP_078065389.1 DNA repair protein RadA Machinery gene
  JN057_RS00430 (JN057_00090) - 62686..63183 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  JN057_RS00440 (JN057_00091) - 63208..64007 (+) 800 Protein_82 PrsW family intramembrane metalloprotease -
  JN057_RS00445 (JN057_00092) - 64152..65120 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  JN057_RS00450 - 65254..65535 (-) 282 Protein_84 transposase family protein -
  JN057_RS00455 - 65662..66569 (-) 908 Protein_85 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49466.97 Da        Isoelectric Point: 6.5137

>NTDB_id=479045 JN057_RS00425 WP_078065389.1 61252..62613(+) (radA) [Streptococcus pneumoniae strain BVJ1JL]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFFIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=479045 JN057_RS00425 WP_078065389.1 61252..62613(+) (radA) [Streptococcus pneumoniae strain BVJ1JL]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAATTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTCTTCATTATTGACTCTATCCAGACCATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTAGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAACCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA/sms Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631