Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IAQ69_RS02260 Genome accession   NZ_CP060811
Coordinates   490643..491416 (+) Length   257 a.a.
NCBI ID   WP_373685615.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain XM9F202-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485643..496416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAQ69_RS02235 (IAQ69_02235) ilvC 485681..486697 (+) 1017 WP_005232140.1 ketol-acid reductoisomerase -
  IAQ69_RS02240 (IAQ69_02240) - 487862..488413 (+) 552 WP_004782094.1 phosphatase PAP2 family protein -
  IAQ69_RS02245 (IAQ69_02245) iscX 488502..488702 (+) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  IAQ69_RS02250 (IAQ69_02250) ndk 488813..489244 (+) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  IAQ69_RS02255 (IAQ69_02255) rlmN 489366..490601 (+) 1236 WP_200229730.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IAQ69_RS02260 (IAQ69_02260) pilF 490643..491416 (+) 774 WP_373685615.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IAQ69_RS02265 (IAQ69_02265) - 491407..492192 (+) 786 WP_200229732.1 helix-turn-helix domain-containing protein -
  IAQ69_RS02270 (IAQ69_02270) ispG 492216..493337 (+) 1122 WP_004782104.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IAQ69_RS02275 (IAQ69_02275) hisS 493341..494636 (+) 1296 WP_180052460.1 histidine--tRNA ligase -
  IAQ69_RS02280 (IAQ69_02280) - 494667..495365 (+) 699 WP_005232125.1 YfgM family protein -

Sequence

Protein


Download         Length: 257 a.a.        Molecular weight: 29017.96 Da        Isoelectric Point: 8.7640

>NTDB_id=478526 IAQ69_RS02260 WP_373685615.1 490643..491416(+) (pilF) [Acinetobacter variabilis strain XM9F202-2]
MVAVVCGSLVLTACQTPDLGQKDPEKATKVRTQLAVEYLKNGDLDSAKRALDQALETNPRDSQANMMMGVLLQQEGSKPN
LEKAEGYFKRAISADPKNAQARNNYGTYLYQLERYKDAIEQFEIAGATLGYDQRFRALENMGRIYLKQGDLANAEKSFKQ
ALQANRASYISMLELAEIFYLNQQFPAATQMYEQFVRGVGQKNQSARALWIGIRTARASGDQLGMQVLVNQLRALFPESP
EYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 774 bp        

>NTDB_id=478526 IAQ69_RS02260 WP_373685615.1 490643..491416(+) (pilF) [Acinetobacter variabilis strain XM9F202-2]
TTGGTTGCAGTAGTATGTGGGTCTTTGGTTTTAACTGCGTGTCAAACGCCAGATCTAGGTCAGAAGGACCCGGAAAAAGC
CACCAAGGTTCGTACTCAGTTGGCGGTTGAATATCTTAAAAACGGTGATCTGGATTCAGCTAAACGTGCGCTGGATCAGG
CACTGGAAACTAATCCGCGTGATTCGCAAGCCAATATGATGATGGGCGTGCTGCTTCAGCAGGAAGGCAGCAAACCTAAT
CTTGAAAAGGCCGAAGGTTATTTCAAGCGTGCAATTTCGGCAGATCCAAAAAATGCCCAGGCACGAAATAACTATGGCAC
CTATTTATATCAGCTTGAGCGCTATAAAGATGCGATCGAGCAGTTCGAAATTGCCGGCGCAACTTTAGGTTATGACCAGC
GATTTCGTGCCCTTGAAAATATGGGACGGATTTACCTGAAACAGGGTGATCTGGCGAATGCTGAAAAATCATTCAAACAG
GCACTACAGGCTAATCGCGCTTCGTATATATCAATGTTAGAGTTGGCAGAAATTTTTTATCTGAACCAGCAGTTCCCGGC
CGCGACTCAAATGTATGAACAGTTTGTACGTGGCGTGGGGCAGAAAAACCAGAGTGCCCGTGCGCTCTGGATTGGCATTC
GCACTGCGCGTGCCAGTGGCGATCAGTTAGGCATGCAGGTATTGGTGAATCAATTACGTGCATTGTTCCCTGAAAGCCCG
GAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(99-166)

Tetratricopeptide repeat (TPR_14)

(31-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.447

98.444

0.782
  pilF Acinetobacter baylyi ADP1

71.875

99.611

0.716