Detailed information NTG0478410

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: H9S87_RS05000
• Genome accession: NZ_CP060799
• Coordinates: 1041001..1044705 (+)
• Length: 1234 a.a.
• NCBI ID: WP_187704438.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain ONU 554
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1036001..1049705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9S87_RS04985 (H9S87_04985)	-	1036293..1036703 (+)	411	WP_034663477.1	DoxX family protein	-
H9S87_RS04990 (H9S87_04990)	-	1036900..1037346 (-)	447	WP_034663474.1	ferritin-like domain-containing protein	-
H9S87_RS04995 (H9S87_04995)	addB	1037514..1041023 (+)	3510	WP_034663471.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
H9S87_RS05000 (H9S87_05000)	addA	1041001..1044705 (+)	3705	WP_187704438.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
H9S87_RS05005 (H9S87_05005)	sbcD	1044732..1045907 (+)	1176	WP_034663467.1	exonuclease subunit SbcD	-
H9S87_RS05010 (H9S87_05010)	-	1045904..1049299 (+)	3396	WP_034663465.1	AAA family ATPase	-
H9S87_RS05015 (H9S87_05015)	-	1049319..1049618 (+)	300	WP_034663464.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142493.44 Da
• Isoelectric Point: 5.0448

>NTDB_id=478410 H9S87_RS05000 WP_187704438.1 1041001..1044705(+) (addA) [Bacillus pumilus strain ONU 554]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPLPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIHHQDDFEELYELLPNVNFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPSQHLKSLAEMKPVVETLVELVIKFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAESDEEREELETVQFEARIIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLHKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHITLPSEEPYDESRIRHLLESLQQRDLLTDEQVRSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLDAAESILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=478410 H9S87_RS05000 WP_187704438.1 1041001..1044705(+) (addA) [Bacillus pumilus strain ONU 554]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGAAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATTACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCCCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGTGCTAATATTTC
TACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGACCCAGGCTTTCGTTTGGCAG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTCGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGCCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCTAGGTCTCATCCTTTACCTGAGCAATGGATGAGGGCATTCCTTTCACTTTATGATGTAGATGCACAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCATCATCAAGATGATTTTGAGGAGCTTTACGAACTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGATGAATATGACCCTGTTTTGTTAGAAAAGGCCACAGATGCACGTAATCAAGCAAAAAAACAACTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCTTCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCAAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATCGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTTGTTGATGAATACCAGGATACAAACCTCGTACAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGA
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTCCTTGTCCACTTAG
ATCAGCAGGAAGCGGAAAGCGACGAAGAGCGAGAAGAACTTGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAAGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCGTATCAG
GATATTCCGCTTGCAGCTGTTTTAAGATCACCTATTGTTCATTTAGATGAAAATGAAATGGCACTTATTCGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCAGATCACCCTACATGCAAAAAATTAG
AGCGATTTTTCCAATTGCTGCATAAGTGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGAGATACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
TCGAGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTTCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACTGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTCCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCGACTGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCATCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGCGATGTCGTTCTTTCGCATCCGTCCACATTCACCATCTCATTTACTCAAGCTTCTGATCTT
CTAAAGGAAGATATGTCACTTGAAAAGAAGCAGCAAGACGAGGTAGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTTTCATGGAAATATCCATATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGGATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
CATGCAGCATATCACGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAAGGCACCTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAAGTCCGGTCAATTGATCAAGAAGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTGAAAGCGGACTGGGTAAAACGAGAAGTATCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAGACGGATAGAGTGCAAGGCAGATATACAGGTGGACTTGACGCTGCTGAATCTATCTTGAAAAAGCGCTATGAA
ACCCAAATAGCGTTATATGCAAAAGCAGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.343	100	0.665